The production of hydrolysates from industrially defatted rice bran and its surface image changes during extraction

Kaewjumpol, Geerada; Oruña-Concha, Marı́a José; Niranjan, Keshavan; Thawornchinsombut, Supawan

doi:10.1002/jsfa.8832

Cited by 20 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The major hurdle in the utilization of these materials as fermentation feedstock is that they cannot be used in their current states by many microorganisms, and instead they require hydrolysis to convert them into simple fermentable sugars. Rice and wheat are among the top three crops worldwide, and their utilization is very low despite the high production of agricultural byproducts [15]. The goal of this study was to determine a means of using these discarded agricultural byproducts for the production of poly-(γ-glutamic acid).…”

Section: Optimization Using One-factor-at-a-timementioning

confidence: 99%

Poly-(γ-glutamic acid) Production and Optimization from Agro-Industrial Bioresources as Renewable Substrates by Bacillus sp. FBL-2 through Response Surface Methodology

et al. 2019

View full text Add to dashboard Cite

We optimized culture conditions using Bacillus sp. FBL-2 as a poly-(γ-glutamic acid) (PGA) producing strain isolated from cheonggukjang. All experiments were performed under aerobic conditions using a laboratory scale 2.5 L fermentor. We investigated the effects of fermentation parameters (temperature, pH, agitation, and aeration) and medium components (glutamic acid, citric acid, and yeast extract) on poly-(γ-glutamic acid) production, viscosity, and dry cell mass. A non-optimized fermentation method (1.5 vvm, 350 rpm, and 37 °C) yielded PGA, viscosity, and dry cell mass at levels of 100.7 g/L, 483.2 cP, and 3.4 g/L, respectively. L-glutamic acid, citric acid, and yeast extract supplementation enhanced poly-(γ-glutamic acid) production to 175.9 g/L. Additionally, the production of poly-(γ-glutamic acid) from rice bran and wheat bran was assessed using response surface methodology (central composite rotatable design). Agricultural byproducts (rice bran and wheat bran) and H2SO4 were selected as factors, and experiments were performed by combining various component concentrations to determine optimal component concentrations. Our experimentally-derived optimal parameters included 38.6 g/L of rice bran, 0.42% of H2SO4, 28.0 g/L of wheat bran, and 0.32% of H2SO4. Under optimum conditions, rice bran medium facilitated poly-(γ-glutamic acid) production of up to 22.64 g/L, and the use of wheat bran medium yielded up to 14.6 g/L. Based on a validity test using the optimized culture conditions, poly-(γ-glutamic acid) was produced at 47.6 g/L and 36.4 g/L from these respective mediums, and both results were higher than statistically predicted. This study suggests that rice bran can be used as a potential alternative substrate for poly-(γ-glutamic acid) production.

show abstract

Section: Optimization Using One-factor-at-a-timementioning

confidence: 99%

Poly-(γ-glutamic acid) Production and Optimization from Agro-Industrial Bioresources as Renewable Substrates by Bacillus sp. FBL-2 through Response Surface Methodology

et al. 2019

View full text Add to dashboard Cite

show abstract

“…These properties are appealing for use in natural ACE inhibitors for hypertensive patients. Our previous studies revealed that peptides‐derived from Thai jasmine rice bran also have strong in vitro antioxidant and ACE inhibitory activities (Timachai, ; Kaewjumpol & Thawornchinsombut, ; Kaewjumpol et al , ). Results from in vivo studies of peptides from cold‐pressed defatted Thai jasmine rice bran exhibited beneficial effects on antihypertensive properties by inhibiting ACE and vasodilatory and antioxidant activities (Boonla et al , ), antihyperglycemic effects by improving insulin sensitivity and precluding diabetic nephropathy (Boonloh et al , ; Boonloh et al , ), as well as the improvement of metabolic changes and hemodynamic status, and a reduction in aortic stiffening, vascular dysfunction, inflammation and oxidative stress (Senaphan et al , ).…”

Section: Introductionmentioning

confidence: 99%

Angiotensin‐I‐converting enzyme (ACE)‐inhibitory peptides from Thai jasmine rice bran protein hydrolysates

Suwannapan

Wachirattanapongmetee

Thawornchinsombut

et al. 2020

Int J of Food Sci Tech

Self Cite

View full text Add to dashboard Cite

Rice bran protein hydrolysate (<50 kDa RBPH) from Thai jasmine variety demonstrating a high Angiotensin I converting enzyme (ACE) inhibitory activity was purified and characterised. ACE inhibitory peptides were obtained from a two-step purification process: gel filtration and preparative reverse-phase high-performance chromatography (RP-HPLC) and then identified by mass spectrometer hybrid quadrupole-time-of-flight. A novel peptide GSGYF in the RBPH was firstly identified and found to have a partial sequence homology of Oryza sativa Japonica Group. This sequence was further synthesised to exhibit as good an inhibition potency with IC 50 value of 2.11 µg mL À1 as Captopril (1.15 µg mL À1 ). The cytotoxicity test revealed that this RBPH is non-toxic against Vero cells. In addition, the <50 kDa RBPH was resistant to in vitro digestion by pepsin and trypsin. These findings suggest that the RBPH containing ACE inhibitory peptides is likely to be safer and healthier than synthetic drugs and can be an effective food supplement for lowering blood pressure.

show abstract

“…Notably, DEP-induced cell proliferation is inhibited by autophagic inhibitor 3-MA and chloroquine but not by COX-2 inhibitor. “Etoricoxib” again pointed toward different mechanisms of autophagy ( Kaewjumpol et al, 2018 ). Chloroquine and 3-MA show inhibition of cell proliferation in a number of cell types, providing support to the present findings ( Fan et al, 2006 ; Jiang et al, 2008 ; Dong et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Proliferation of Lung Epithelial Cells Is Regulated by the Mechanisms of Autophagy Upon Exposure of Soots

Niranjan

Mishra

Tripathi

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

BackgroundSoots are known to cause many diseases in humans, but their underlying mechanisms of toxicity are still not known. Here, we report that soots induce cell proliferation of lung epithelial cells via modulating autophagy pathways.ResultsFullerene soot and diesel exhaust particles (DEP) induced cell proliferation of lung epithelial, A549 cells via distinct autophagic mechanisms and did not cause cell death. Exposure of fullerene soot protected the cell death of A549 cells, caused by hydrogen peroxide, and inhibited LPS-induced autophagy. Fullerene soot co-localized with the autophagic proteins and inhibited starvation-induced autophagy (downregulated ATG-5, beclin-1, p62, and LC3 expressions) independent of its antioxidant properties. Similarly, it decreased the expression profile of autophagic genes and upregulated the proliferation-responsive gene, Ki-67, in mice. We observed that expressions of fullerene soot-responsive genes (Beclin-1, ATG-5, and p62) were reverted by Akt Inhibitor X, indicating an important role of the Akt pathway. At an elemental level, we found that elemental carbon of fullerene soot may be converted into organic carbon, as measured by OCEC, which may point fullerene soot as a source of carbon. On the other hand, DEP upregulated the expressions of autophagy genes. Akt Inhibitor X did not attenuate DEP-induced cell proliferation and autophagic response. However, an autophagic inhibitor, chloroquine, and significantly inhibited DEP-induced cell proliferation.ConclusionIt can be said that distinct autophagic mechanisms are operational in cell proliferation of lung epithelial cells due to soots, which may be responsible for different diseases. Understanding the mechanism of these pathways provides some important targets, which can be utilized for the development of future therapeutics.

show abstract

The production of hydrolysates from industrially defatted rice bran and its surface image changes during extraction

Abstract: The process of mild-SAW extraction followed by proteolysis promotes the release of active compounds from IDRB. © 2017 Society of Chemical Industry.

Cited by 20 publications

References 36 publications

Poly-(γ-glutamic acid) Production and Optimization from Agro-Industrial Bioresources as Renewable Substrates by Bacillus sp. FBL-2 through Response Surface Methodology

Poly-(γ-glutamic acid) Production and Optimization from Agro-Industrial Bioresources as Renewable Substrates by Bacillus sp. FBL-2 through Response Surface Methodology

Angiotensin‐I‐converting enzyme (ACE)‐inhibitory peptides from Thai jasmine rice bran protein hydrolysates

Proliferation of Lung Epithelial Cells Is Regulated by the Mechanisms of Autophagy Upon Exposure of Soots

Contact Info

Product

Resources

About