The Production of Reactive Oxygen Species in Human Erythrocytes during Cryopreservation with Glycerol and Polyethylene Glycol

Zemlianskykh, N. G.; Babiychuk, L. A.

doi:10.1134/s0006350919040237

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…In the present study, each glycerol concentration tested caused a significant increase in the production of intracellular ROS, which was also positively correlated with hemolysis. In other studies, incubation of RBCs with glycerol was not associated with an increase in RBCs' ROS production [49]. The concentration used in the above-mentioned study (3.5 mg/dL) [49] was, however, significantly lower than the ones tested in the present one.…”

Section: Discussioncontrasting

confidence: 61%

“…In other studies, incubation of RBCs with glycerol was not associated with an increase in RBCs' ROS production [49]. The concentration used in the above-mentioned study (3.5 mg/dL) [49] was, however, significantly lower than the ones tested in the present one. The increase in RBCs' ROS production found in the present study may thus be linked directly to glycerol entry in the cells, to the alterations in cell volume, or to a combination of both.…”

Section: Discussioncontrasting

confidence: 61%

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Effect of Media with Different Glycerol Concentrations on Sheep Red Blood Cells’ Viability In Vitro

Pasciu

Sotgiu

Porcu

et al. 2021

Animals

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The use of high doses of glycerol as a livestock feed supplement is followed by a rapid increase in plasma concentrations and consequently in plasma osmolality. Moreover, glycerol is a highly diffusible molecule that can readily permeate the red blood cell (RBC) membrane following a concentration gradient. A rise in glycerol plasma concentrations can thus alter RBC homeostasis. The present study aimed at investigating both glycerol osmotic effects on sheep RBCs and their oxidative response under in vitro conditions. Sheep blood samples were suspended in media supplemented with increasing glycerol concentrations (0, 25, 50, 100, 150, 200, 250, 300, 350, 400 mg/dL), which reflected those found in vivo in previous studies, and incubated at 37 °C for 4h. Thereafter, osmolality and hemolysis were determined in spent media, while cell extracts were used to assay intracellular concentration of glycerol, ATP, Ca2+ ions, oxidative stress markers and reactive oxygen species (ROS).The study confirmed that glycerol intracellular concentrations are directly related with its concentration in the incubation media, as well as hemolysis (p < 0.001) which increased significantly at glycerol concentrations higher form 200 mg/dL. ROS intracellular level increased at all glycerol concentration tested (p < 0.01) and total thiols decreased at the highest concentrations. However, RBCs proved to be able to cope by activating their antioxidant defense system. Superoxide dismutase activity indeed increased at the highest glycerol concentrations (p < 0.001), while total antioxidant capacity and malonyldialdehyde, a typical product of lipid peroxidation by ROS, did not show significant changes. Moreover, no alterations in intracellular Ca2+ ions and ATP concentrations were found. In conclusion, glycerol-induced hemolysis can be related to the induced osmotic stress. In sheep, nutritional treatments should be designed to avoid reaching glycerol circulating concentrations higher than 200 mg/dL.

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Section: Discussioncontrasting

confidence: 61%

Section: Discussioncontrasting

confidence: 61%

Effect of Media with Different Glycerol Concentrations on Sheep Red Blood Cells’ Viability In Vitro

Pasciu

Sotgiu

Porcu

et al. 2021

Animals

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Cold in Biology and Medicine: state of the art and perspectives

Edited by Professor O. Yu. Petrenko

2023

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The monograph highlights the main development stages of the Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine. It provides an infor-mation about the major achievements in cryobiology and cryomedicine, fundamental and applied researches being of a priority value. Certain scientific directions formed during this time and coi-ned as the separate scientific schools have been specially empasized. Particular attention is paid to the basic aspects of cryopreservation of biological objects, mechanisms of cold adaptation of biolo-gical systems, modern methodical approaches to cryopreservation of genetic resources and stem cells of various origins, development of low-temperature techniques in modern biology and regenerative medicine. The monograph is intended to biologists and medical specialists, biology and medicine students, those having an interest in modern cryotechniques in biology and medicine.

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Polyethylene Glycols Stimulate Ca2+ Signaling, Cytokine Production, and the Formation of Neutrophil Extracellular Traps

Hinz,

Stankiewicz,

Litewka

et al. 2024

IJN

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Purpose Given the increased use of polyethylene glycol (PEG) in refining the therapeutic activity of medicines, our research focuses on explaining the potential mechanism of immune reactions associated with this polymer. We aim to investigate the interaction of different types of PEG with mouse and human immune cells, thereby contributing to understanding PEG interactions with the immune system and verifying the proinflammatory activity of the tested polymers. Patients and Methods Mouse macrophage and neutrophil cell lines, human peripheral blood mononuclear cells, and polymorphonuclear cells isolated from healthy donors were exposed to various PEGs. ROS, NO, and cytokine production were analyzed using fluorescence intensity, absorbance, or cytometric measurements. Toll-like receptor (TLR) signaling was verified using HEK-blue-reporter cell lines. Finally, neutrophil trap formation was studied using immunofluorescence labeling, and calcium imaging was performed using a Ca 2+ -sensitive indicator and fluorescence microscope. Results Our findings show that specific PEG and mPEG are not toxic to tested mouse and human cells. However, they exert proinflammatory activity against human immune cells, as evidenced by the increased secretion of proinflammatory cytokines, such as IFN-a2, IFN-γ, TNF-α, MCP-1, IL-8, IL-17A, and IL-23. This phenomenon is independent of PEG signaling via TLR. Additionally, mPEG10 induced the formation of neutrophil extracellular traps and intracellular calcium signaling. Conclusion Our finding suggests that some PEG types have proinflammatory activity against human immune cells, manifesting in the upregulated production of cytokines and neutrophils trap releasing.

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The Production of Reactive Oxygen Species in Human Erythrocytes during Cryopreservation with Glycerol and Polyethylene Glycol

Cited by 4 publications

References 39 publications

Effect of Media with Different Glycerol Concentrations on Sheep Red Blood Cells’ Viability In Vitro

Effect of Media with Different Glycerol Concentrations on Sheep Red Blood Cells’ Viability In Vitro

Cold in Biology and Medicine: state of the art and perspectives

Polyethylene Glycols Stimulate Ca2+ Signaling, Cytokine Production, and the Formation of Neutrophil Extracellular Traps

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