The professional phagocytes of sea bass (Dicentrarchus labrax L.): cytochemical characterisation of neutrophils and macrophages in the normal and inflamed peritoneal cavity

Vale, Ana do; Afonso, António; Silva, Manuel T.

doi:10.1006/fsim.2001.0394

Cited by 89 publications

(77 citation statements)

References 26 publications

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“…Tentativas de se adotar nomenclatura própria para hematologia de peixes foram feitas inicialmente por Jordan (1938) Nos mamíferos (LORENZI, 1999) e em peixes (VALE et al, 2002) os neutrófilos são células fagocíticas com importante papel na defesa contra infecções (THUVANDER et al, 1978;VALE et al, 2002). Estudos com diversas espécies de peixes demonstram a capacidade fagocítica dos neutrófilos sanguíneos e / ou teciduais ao englobar partículas antigênicas e bactérias (THUVANDER et al, 1978;AFONSO et al 1997;AFONSO et al, 1998;VALE et al, 2002).…”

Section: Resultados (Tavares-dias and Moraes 2004)unclassified

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Estresse de transporte em cascudos amazônicos ornamentais Cochliodon sp. (L145) e Hypostomus sp. (L28) (LORICARIIDAE)

et al. 2016

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In this study was evaluated the hematological response of ornamental Amazon plecos Cochliodon sp. (L145) and Hypostomus sp. (L28) submitted to transportation conditions. The erythrogram, leukogram, thrombogram, glucose and total plasma proteins (PPT) were determined at 0, 6, 24, 48 and 72 hours after 3-hours simulated transportation. For basal hematological profile, the blood was collected immediately to allow comparisons after the transport stress. Cochliodon sp. showed reduction in total erythrocyte after 6 and 24 hours of post-transport, and increase after 6 hours. Hypoglycemia were observed at 6 hours post-transport for Hypostomus sp. Thus, the Hypostomus sp. showed higher resistance compared to Cochliodon sp. when to the transport stress.

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Section: Resultados (Tavares-dias and Moraes 2004)unclassified

“…Estudos com diversas espécies de peixes demonstram a capacidade fagocítica dos neutrófilos sanguíneos e / ou teciduais ao englobar partículas antigênicas e bactérias (THUVANDER et al, 1978;AFONSO et al 1997;AFONSO et al, 1998;VALE et al, 2002).…”

Section: Resultados (Tavares-dias and Moraes 2004)unclassified

Estresse de transporte em cascudos amazônicos ornamentais Cochliodon sp. (L145) e Hypostomus sp. (L28) (LORICARIIDAE)

et al. 2016

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“…Cells were fixed, stained for myeloperoxidase and nonspecific esterase, and counterstained with hematoxylin and methylene blue, respectively, according to the manufacturer's instructions (Sigma-Aldrich; procedure 181-B and 390). Macrophages/ Monocytes from mammals and fish can be identified by positive staining for esterase and negative staining for myeloperoxidase, while granulocytes stain positive for myeloperoxidase and negative for esterase (30,31).…”

Section: Histochemical Analyses Of the Tc5a-responsive Cellsmentioning

confidence: 99%

A Functional C5a Anaphylatoxin Receptor in a Teleost Species

Holland

Lambris

2004

The Journal of Immunology

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The anaphylatoxins are potent, complement-derived low m.w. proteins that bind to specific seven-transmembrane receptors to elicit and amplify a variety of inflammatory reactions. C5a is the most potent of these phlogistic peptides and is a strong chemoattractant for neutrophils and macrophages/monocytes. Although lower vertebrates possess complement systems that are believed to function similarly to those of mammals, anaphylatoxin receptors have not previously been characterized in any nonmammalian vertebrate. To study the functions of C5a in teleost fish, we generated recombinant C5a of the rainbow trout, Oncorhynchus mykiss (tC5a), and used fluoresceinated tC5a (tC5aF) and flow cytometry to identify the C5a receptor (C5aR) on trout leukocytes. Granulocytes/Macrophages present in cell suspensions of the head kidney (HKL), the main hemopoietic organ in teleosts, showed a univariate type of receptor expression, whereas those from the peripheral blood demonstrated either a low or high level of expression. The binding of tC5aF was inhibited by excess amounts of unlabeled tC5a or tC5adesArg, demonstrating that sites other than the C-terminal of tC5a interact with the C5aR. Both tC5a and tC5adesArg were able to induce chemotactic responses in granulocytes in a concentration-dependent manner, but the desArg derivative was at least 10-fold less active. Homologous desensitization occurred after HKL were exposed to continuous or high concentrations of tC5a, with a loss of tC5aF binding and an 80% reduction in chemotactic responses toward tC5a. Pertussis toxin reduced the migration of HKL toward tC5a by 40%, suggesting only a partial involvement of pertussis toxin-sensitive Gi proteins in tC5a-mediated chemotaxis.

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“…On the other hand, triploid jundia showed a significant increase in the circulating neuthrophyls and monocytes, likely to keep its defense system more prepared for adverse rearing conditions. These cells play an important role in the fish immune system against the microorganisms being sensitive to migration to the inflammatory site (Vale et al, 2002;Tavares Dias and Moraes, 2004).…”

Section: Discussionmentioning

confidence: 99%

Triploidy in the hematology of jundia juveniles (Siluriformes: Heptapteridae)

et al. 2012

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This study compared the hematological characteristics of diploid and triploid of jundia, Rhamdia quelen juveniles, an important freshwater fish cultured in south Brazil. Hematological morphometry of erythrocytes were determined in blood smears under a light microscope. The blood was used to measure the number of red blood cells (RBC) with a hemocytometer Neubauer chamber, and the numbers of white blood cells (WBC) and thrombocytes that were obtained using an indirect method. The results showed that triploidy increased (p < 0.01) the size and volume of the erythrocytes. Nevertheless, as expected, triploidy decreased (p < 0.01) the number of circulating erythrocytes, leucocytes and trombocytes in the blood of jundia. Moreover differential cell counts were different in diploids and triploids, suggesting that triploidy affects the number of cells and their proportion in blood. Lymphocytes were the most predominant cells in the differential counting of diploid fish (62.5%) while monocytes were predominant in triploid fish (49.6%). These results suggest performance differences between ploidies of jundia, and require future studies to evaluate the potential of triploid jundia in the culture conditions and resistance to infection.Keywords: Rhamdia quelen, triploid, diploid, blood cells. Triploidia na hematologia de juvenis de jundiá (Siluriformes: Heptapteridae)Resumo O presente estudo comparou características hematológicas de juvenis de jundiás Rhamdia quelen diploides e triploides, um importante peixe de água doce cultivado no sul do Brasil. A morfometria hematológica dos eritrócitos foi determinada em extensões sanguíneas sob microscopia óptica comum. O sangue dos animais foi também utilizado para mensurar o número total de eritrócitos em câmera de Neubauer, bem como os números totais de leucócitos e trombócitos pelo método indireto. Os resultados demonstraram que a triploidia aumentou (p < 0,01) o tamanho e volume dos eritrócitos. No entanto, como esperado, a triploidia reduziu (p < 0,01) o número de eritrócitos, leucócitos e trombócitos circulantes do jundiá. Além disto, a contagem diferencial de células sanguíneas foi diferente em diploides e triploides, sugerindo que a triploidia afeta o número das células, bem como sua proporção no sangue. Linfócitos foram as células predominantes na contagem de leucócitos de peixes diploides (62,5%), enquanto os monócitos foram os predominantes nos peixes triploides (49,6%). Estes resultados sugerem desempenhos diferentes entre as ploidias do jundiá e exige estudos futuros para avaliar o potencial dos jundiás triploides sob condições de cultivo e frente à infecção.Palavras-chave: Rhamdia quelen, triploide, diploide, células sanguíneas.

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The professional phagocytes of sea bass (Dicentrarchus labrax L.): cytochemical characterisation of neutrophils and macrophages in the normal and inflamed peritoneal cavity

Cited by 89 publications

References 26 publications

Estresse de transporte em cascudos amazônicos ornamentais Cochliodon sp. (L145) e Hypostomus sp. (L28) (LORICARIIDAE)

Estresse de transporte em cascudos amazônicos ornamentais Cochliodon sp. (L145) e Hypostomus sp. (L28) (LORICARIIDAE)

A Functional C5a Anaphylatoxin Receptor in a Teleost Species

Triploidy in the hematology of jundia juveniles (Siluriformes: Heptapteridae)

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