Standard precautions is an action to prevent infection in any cases of medical treatment toward patients. However, some studies have shown that the implementation of standard precautions has not been optimal due to the barriers perceived by nurses. This study aimed to identify the barriers perceived by nurses in the implementation of standard precautions. Descriptive-quantitative method was used with cross-sectional approach in 37 nurses in General Hospital Emergency Department by using total sampling technique. The data were collected by using questionnaires consisting of 48 statements, including five action of standard precautions (hand hygiene, use of PPEs, safe injection, environmental disinfection, and respiratory hygiene practices/ethics of cough and sneezing) and have been through the content and face validity test. The data were analyzed by using frequency distribution and presented in percentage. The results showed that there were some barriers perceived by nurses on standard precautions action. The most perceived barriers are limited PPEs equipment (45.9%), equipment that irritates the skin on hand hygiene (37.8%), increased number of patients in environmental disinfection (32.4%), limited support and supervision in the implementations of standard precautions for safe injection (16.2%), and equipment limitations on respiratory hygiene practices/ethics of cough and sneezing (10.8%). Based on this research, the hospital should strengthen the function properly and regularly to overcome some equipment, seek hand hygiene equipment containing emollient/moisturizers and alcohol type hand rub n-propanol to overcome skin irritation, and pay attention to the study of the ratio of nurses and patients to safe working practices. In addition, the hospital should create a practice program, socialize rewards or penalties to improve it in standard precautions.