The profiling and identification of chemical components, prototypes and metabolites of Run‐zao‐zhi‐yang capsule in rat plasma, urine and bile by an UPLC‐Q‐TOF/MS^E‐based high‐throughput strategy

Abstract

Run-zao-zhi-yang (RZZY) capsule, a traditional Chinese medicine formula, is popularly used for the treatment of dermatitis and eczema. However, few studies have been carried out on RZZY and its metabolites. In this study, we developed a three-step strategy to rapidly characterize the chemical constituents and metabolites of RZZY using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. A total of 41 chemical components were characterized from RZZY. Among these, there are 11 flavonoids, six alkaloids, six stilbene glycosides, five anthraquinones and 13 other compounds. In addition, 18 prototypes and 35 metabolites were detected in rat plasma, urine and bile. This study offers an applicable approach for high-throughput profiling and identification of chemical components and metabolites derived from traditional Chinese medicine formula in vivo, and also provides essential data for exploring bioactive ingredients and action mechanisms of RZZY.

Identification of Antitumor Active Constituents in Polygonatum sibiricum Flower by UPLC-Q-TOF-MSE and Network Pharmacology

“…Moreover, it can provide good separation and enable rapid and efficient analysis of some complex components. Furthermore, UPLC-Q-TOF-MS E has been widely used in related fields, such as TCM component analysis and metabolomics . In this study, we used UPLC-Q-TOF-MS E combined with the UNIFI platform for the rapid qualitative analysis of the chemical composition of P.…”

“…Furthermore, UPLC-Q-TOF-MS E has been widely used in related fields, such as TCM component analysis and metabolomics. 9 In this study, we used UPLC-Q-TOF-MS E combined with the UNIFI platform for the rapid qualitative analysis of the chemical composition of P. sibiricum flower. The results will provide a reference for quality control and material basis for the efficacy of P. sibiricum.…”

Data_Sheet_1.docx

“…The UPLC-MRM/MS and other chromatographic conditions, modified from previous published protocols, were used for the quantitative analysis (Lin et al, 2018;Khoomrung et al, 2020). A 5-µL aliquot of standards or samples was injected into a reversed-phase column (Waters CSH C 18 , 2.1 × 100 mm, 1.7 µM column; MA, United States).…”