The Prognostic Impact of p53 Expression on Sporadic Colorectal Cancer Is Dependent on p21 Status

Abstract

The prognostic value of p53 and p21 expression in colorectal cancer is still under debate. We hypothesize that the prognostic impact of p53 expression is dependent on p21 status. The expression of p53 and p21 was immunohistochemically investigated in a prospective cohort of 116 patients with UICC stage II and III sporadic colorectal cancer. The results were correlated with overall and recurrence-free survival. The mean observation period was 51.8 ± 2.5 months. Expression of p53 was observed in 72 tumors (63%). Overall survival was significantly better in patients with p53-positive carcinomas than in those without p53 expression (p = 0.048). No differences were found in recurrence-free survival (p = 0.161). The p53+/p21− combination was seen in 68% (n = 49), the p53+/p21+ combination in 32% (n = 23). Patients with p53+/p21− carcinomas had significantly better overall and recurrence-free survival than those with p53+/p21+ (p < 0.0001 resp. p = 0.003). Our data suggest that the prognostic impact of p53 expression on sporadic colorectal cancer is dependent on p21 status.

Predictive clinical model of tumor response after chemoradiation in rectal cancer

“…Nevertheless, some studies show agreeing results with ours [ 51 , 61 , 62 ] and others don’t, probably due to the selected technique. Immunohistochemistry is highly dependent on the antibody clone that is used, staining protocols, selection of scoring methods and nuclear/cytoplasmic staining account .…”

Improved potency of F10 relative to 5-fluorouracil in colorectal cancer cells with p53 mutations

“…TP53 mutational status affects inherent sensitivity to FPs, with p53 GOF mutations most deleterious. F10 is much more effective than 5-FU regardless of TP53 mutations and has potential to be effective to CRC that is resistant to 5-FU due, in part, to TP53 mutations 6,7…”

Validation of the systematic scoring of immunohistochemically stained tumour tissue microarrays using QuPath digital image analysis

“…Immunoexpression data for p53 has been presented and analysed in this study in a continuous scale, consistent with the approach taken in previous studies . This suits the focus of this study, which is comparability of digital image analysis scoring by QuPath among different reviewers.…”

“…Immunoexpression data for p53 has been presented and analysed in this study in a continuous scale, consistent with the approach taken in previous studies. 10,16,17 This suits the focus of this study, which is comparability of digital image analysis scoring by QuPath among different reviewers. However, given recent developments in the understanding of p53 biology, and the relationship between different p53 mutations and immunoexpression patterns, it is now considered more appropriate to analyse p53 staining by comparing normal or 'wild-type' staining with aberrant extremes of staining ('mutation type').…”