The prognostic significance and immune correlation of SLC10A3 in low-grade gliomas revealed by bioinformatic analysis and multiple immunohistochemistry

Abstract

Purpose: Despite the fact that genetic risk factors contribute to low-grade gliomas (LGGs), the role of critical genes as prognostic and theraputic biomarkers is quite limited. This study is designed to comprehensively investigate the prognostic role and predictive ability of solute carrier family 10 member 3 ( SLC10A3 ) for immunotherapy in LGGs. Methods: We analyzed the prognostic value of SLC10A3 from multiple datasets of LGG patients, and explored its immune correlation via multiple algorithms. Finally, we independently confirmed the clinical significance and its immune correlation using the multiplex staining assay of LGG samples on the tissue microarray. Results: SLC10A3 mRNA was up-regulated in LGGs compared with normal brain tissues, and correlated with tumor grade, histological type, IDH wide type and non-codel 1p19q. Up-regulation of SLC10A3 transcription was remarkably associated with shortened overall survival time compared with down-regulation in TCGA, CGGA and Rembrandt datasets, and SLC10A3 exhibited good predictive ability for survival outcomes among LGGs. Correlation analyses showed that SLC10A3 mRNA expression correlates well with the six immune check points and immune cells. When the expression and immune correlation of SLC10A3 at the translational level were verified via multiplex immunohistochemistry, expression of SLC10A3 protein was higher in LGG compared with normal tissues, and expression of SLC10A3 protein was correlated well with macrophage, CD4 + T cell and B cell. Conclusions: Up-regulation of SLC10A3 mRNA is statistically associated with adverse survival outcomes and immune infiltration among LGGs. SLC10A3 might be a reliable survival predictor and a promising immunotherapy target for LGG patients.

The impact of SLC10A3 on prognosis and immune microenvironment in colorectal adenocarcinoma

“…Specifically, SLC10A3 plays a critical role in regulating the immune response in liver cancer, related with stromal CD4 T cells, CD20 B cells, and macrophage [ 9 ]. Upregulation of SLC10A3 mRNA is statistically related with unfavorable survival outcomes and immune infiltration among low-grade gliomas [ 21 ]. Nonetheless, there is currently no literature on SLC10A3 in colorectal cancer.…”

Integrated profiling identifies DXS253E as a potential prognostic marker in colorectal cancer

“…In liver cancer, high DXS253E protein levels were observed to be significantly associated with immune cell infiltration and poor prognosis [ 22 ]. In terms of LGG, higher DXS253E expression levels contribute to programmed cell death and immune infiltration, resulting in poor prognosis of the patients with LGG [ 40 ]. Consistently, Ma et al also found that the upregulation of DXS253E is associated with poor OS in LGG and glioblastoma [ 41 ].…”

“…Tian et al confirmed significant associations between DXS253E expression and CD4 + Tconv and tumor-infiltrating CD20 + B cells via bioinformatics analysis and multispectral imaging techniques in HCC [ 22 ]. Similarly, a LGG study showed that DXS253E expression was associated with macrophage, CD4 + Tconv cell, and B cell levels [ 40 ]. We consistently found that DXS253E levels were associated with the level of multiple immune-related genes and immune cell infiltration in CRC.…”