The prognostic significance of selective nuclear morphometry in urinary bladder carcinoma

Blomjous, C. E. M.; Vos, Winand; Schipper, N. W.; Uyterlinde, Anne M.; Baak, J. P. A.; Voogt, H. J. de; Meijer, C. J. L. M.

doi:10.1016/0046-8177(90)90203-h

Cited by 30 publications

(12 citation statements)

References 17 publications

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“…Progressive increase of nuclear area was seen with increase of histological grade but no significant difference was found between G1 and G2 tumours, in contrast to Blomjous et al (1990), who also found larger values of the mean nuclear area. However, they measured selected large and abnormal nuclei, with values obviously greater than those we obtained by the evaluation of random nuclei.…”

Section: Discussionmentioning

confidence: 79%

“…The current concept is gaining acceptance that the majority of transitional cell carcinomas exists as non-aggressive low grade or aggressive high grade lesions (Jordan et al 1987). Indeed, selective nuclear morphometry and DNA-FCM can separate bladder carcinomas into only two subgroups with different survival rates (Blomjous et al 1989b(Blomjous et al , 1990.…”

Section: Discussionmentioning

confidence: 99%

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Cell proliferation indices, morphometry and DNA flow cytometry provide objective criteria for distinguishing low and high grade bladder carcinomas

Pich

Chiusa

Comino

et al. 1994

Vichows Archiv A Pathol Anat

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Argyrophilic nucleolar organizer region (Ag-NOR) analysis, proliferating cell nuclear antigen (PC-NA/PC10) and MIB-1 immunohistochemistry, nuclear morphometry and DNA flow cytometry have been performed on formalin-fixed, paraffin-embedded biopsies from 50 patients with transitional cell carcinoma of the urinary bladder. The mean AgNOR count was 6.01 for the 17 grade 1 (G1), 7.59 for the 21 G2 and 13.33 for the 12 G3 carcinomas (p < 0.001). The mean PCNA score was 15.03% for G1, 24.04% for G2 and 40.01% for G3 cases (p < 0.001). The mean MIB-1 score was 11.31% for G1, 17.09% for G2 and 34.47% for G3 carcinomas (p < 0.001). The mean nuclear area was 35.53 microns2 for G1, 38.65 microns2 for G2 and 83.62 microns2 for G3 cases (p < 0.001). Aneuploidy rates were significantly higher (91.7%) in G3 than in G2 (42.9%, p < 0.01) or G1 cases (47.1%, p < 0.05) but not different for G1 versus G2 cases (p = 0.94). While many overlaps of values were seen between G1 and G2 tumours, no overlaps were found between G3 and G1/G2 tumours. Significant differences of values were also found between pTa and invasive tumours (p < 0.0001 for AgNOR count and PCNA score; p < 0.001 for MIB-1 score and mean nuclear area; p < 0.01 for DNA ploidy); however many overlaps were seen. Our findings indicate that the quantitative parameters obtained with different methods are associated with histological grade of bladder urotheliomas and may improve the grading reproducibility. In addition, the absence of overlaps between G3 and G2/G1 carcinomas supports the tendency to classify bladder urotheliomas in only two categories of malignancy.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Cell proliferation indices, morphometry and DNA flow cytometry provide objective criteria for distinguishing low and high grade bladder carcinomas

Pich

Chiusa

Comino

et al. 1994

Vichows Archiv A Pathol Anat

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“…Furthermore, 5-year survival rates of 92%-95% were found in diploid transitional cell carcinomas as opposed to 61.4%-62.5% in aneuploid carcinomas [1,2,26]. Numerous DNA analyses showed that grade I transitional cell carcinomas are predominantly diploid and grade III carcinomas are almost exclusively aneuploid.…”

Section: Discussionmentioning

confidence: 96%

“…However, the prognosis of carcinomas with limited invasion and intermediate differentiation showing mixed papillary/solid growth pattern is difficult to predict. Various methods assessing cell proliferation such as thymidine autoradiography, DNA-cytometry and immunohistochemical proliferation assays have been used to subgrade this group of bladder tumours [1,2,3,12,26]. The results of these studies were promising, but there is still a need for simple measures to predict the prognosis of the intermediate group of bladder carcinomas.…”

Section: Introductionmentioning

confidence: 98%

Nucleolar and argyrophilic nucleolar organizer region counts in urothelial carcinomas with special emphasis on grade II tumors

Helpap

Bulatko

1994

Vichows Archiv A Pathol Anat

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Prognostic assessment of bladder carcinomas of intermediate differentiation is difficult. This study therefore investigated the prognostic values of nucleolar status and silver staining of argyrophilic nucleolar organizer regions (AgNORs) in grade II bladder carcinomas. In biopsies from 34 grade II transitional cell carcinomas of the urinary bladder the number of nuclei with nucleoli, the location of nucleoli within the nucleus and the number of AgNORs were determined in 1000 or 200 nuclei per section respectively. Ten biopsies showing normal urothelium, 18 cases with mild to severe atypia, 27 grade I, 34 grade II and 12 grade III transitional cell carcinomas were also studied. Significantly differing nucleolar and AgNOR values were found comparing normal urothelium/grade I carcinomas with severe urothelial atypia/grade III carcinomas. Grade II carcinomas, however, were inhomogeneous. One subgroup had nucleolar and AgNOR values resembling grade I carcinomas while the second group had values similar to those of grade III carcinomas. This subdivision of grade II carcinomas correlates with results reported for DNA-cytometry. The results suggest a subdivision of patients with grade II transitional cell carcinomas into a low risk and high risk group.

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“…[3][4][5][6][7][8][9][10][11][12][13][14][15][16]19 Some of the authors claimed that quantitative morphometric analysis has the capability of measuring nuclear-related variables with objectivity. 11,13,20 Furthermore, it has been reported that morphometric analysis could improve the prediction of prognosis for several malignancies, including carcinomas of the ovary, 21 breast, 22 thyroid, 23 kidney, 24 bladder, 25 stomach, 26 and colon-rectum. 7,[9][10][11][12][13][14][15][16] The NSF determined by nuclear morphometry appeared to have a promising prognostic significance in colorectal carcinoma.…”

Section: Discussionmentioning

confidence: 99%

Absence of Prognostic Value of Nuclear Shape Factor Analysis in Colorectal Carcinoma: Relevance of Interobserver and Intraobserver Variability

Fabio

Shrier

Bégin

et al. 2008

Diseases of the Colon &Amp; Rectum

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The prognostic significance of selective nuclear morphometry in urinary bladder carcinoma

Cited by 30 publications

References 17 publications

Cell proliferation indices, morphometry and DNA flow cytometry provide objective criteria for distinguishing low and high grade bladder carcinomas

Cell proliferation indices, morphometry and DNA flow cytometry provide objective criteria for distinguishing low and high grade bladder carcinomas

Nucleolar and argyrophilic nucleolar organizer region counts in urothelial carcinomas with special emphasis on grade II tumors

Absence of Prognostic Value of Nuclear Shape Factor Analysis in Colorectal Carcinoma: Relevance of Interobserver and Intraobserver Variability

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