The Prognostic Value of GNG7 in Colorectal Cancer and Its Relationship With Immune Infiltration

Fang, Can; Zhong, Rulei; Qiu, Chenyang; Zou, Bingbing

doi:10.3389/fgene.2022.833013

Cited by 6 publications

(5 citation statements)

References 48 publications

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“…G protein subunit γ7 (Gng7), a member of the G protein family, was moderately to highly correlated with the degree of in ltration of CD4 + T cells in the peripheral blood of patients with colorectal cancer. Additionally, GNG7 expression positively correlated with the degree of in ltration of B cells, macrophages, neutrophils and dendritic cells (37). Gnb3 encodes the G protein β3 subunit and is associated with obesity, diabetes,…”

Section: Discussionmentioning

confidence: 99%

A Preliminary Investigation of the Differences in Immune Responses in Different Strains of Mice with Localized Allergic Rhinitis Tolerance Models

Zhang,

Zhu,

Zou

et al. 2024

Preprint

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Background： BALB/c and C57BL/6 are two common mice strains for the study of allergic rhinitis (AR). We previously found that both mice differed in symptoms and immune phenotypes in the local allergy models.This study aimed to systematically compare the immune differences between the two mice in a local tolerance model and explore the underlying mechanisms. Methods: We used ovalbumin (OVA) as a topical nasal drop for 15 weeks in BALB/c and C57BL/6 mice. Allergic symptoms were recorded. Serum-specific OVA antibodies (IgE, IgG1, IgG2a) and cytokines (IL-4, IL-10, and IFN-γ) in the spleen cell culture supernatants were tested. The expression of eosinophils, goblet cells, TGF-β, IL-10, eotaxin, and RNA-seq on the nasal mucosa was examined. Results: Nasal symptoms of BALB/c worsened first and then relieved, whereas C57BL/6 showed continuously worsened symptoms. The serum antibodies levels in BALB/c increased over time. IgE and IgG1 levels in C57BL/6 showed a trend similar to that of BALB/c, while IgG2a levels did not increase throughout the course. IL-4 first increased and then decreased, while IL-10 remained increased in BALB/c, whereasC57BL/6 showed no significant changes. In BALB/c, the expression of eosinophils and eotaxin in the nasal mucosa increased to a certain extent and remained unchanged, whereas TGF-β and IL-10 expression was upregulated. In C57BL/6, the expression of eosinophils and eotaxin was higher than that in BALB/c at the 15th week. The RNA-sequencing results showed that gene expression in BALB/c was upregulated in biological processes, the tolerance and negative regulatory pathway at the 15th week. Gene expression in C57BL/6 is upregulated in cell synthesis- and secretion-related pathways. Conclusion: Local tolerance and systemic sensitisation was successfully induced in BALB/c by long-term stimulation with OVA. However, local tolerance could not be induced in C57BL/6 . The normal function of T regulatory cells is key to local tolerance.

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Section: Discussionmentioning

confidence: 99%

A Preliminary Investigation of the Differences in Immune Responses in Different Strains of Mice with Localized Allergic Rhinitis Tolerance Models

Zhang,

Zhu,

Zou

et al. 2024

Preprint

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“…GNG7 prevented tumorigenesis in lung adenocarcinoma cells by suppressing E2F transcription factor 1 ( 38 ). GNG7 was a promising prognostic biomarker and was related to immune cell infiltration in colorectal cancer ( 39 ). Overall, these GRGs were closely associated with tumor progression, which merits further investigation.…”

Section: Discussionmentioning

confidence: 99%

A novel risk score model based on gamma-aminobutyric acid signature predicts the survival prognosis of patients with breast cancer

et al. 2023

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BackgroundGamma-aminobutyric acid (GABA) participates in the migration, differentiation, and proliferation of tumor cells. However, the GABA-related risk signature has never been investigated. Hence, we aimed to develop a reliable gene signature based on GABA pathways-related genes (GRGs) to predict the survival prognosis of breast cancer patients.MethodsGABA-related gene sets were acquired from the MSigDB database, while mRNA gene expression profiles and corresponding clinical data of breast cancer patients were downloaded from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. Univariate Cox regression analysis was used to identify prognostic-associated GRGs. Subsequently, LASSO analysis was applied to establish a risk score model. We also constructed a clinical nomogram to perform the survival evaluation. Besides, ESTIMATE and ssGSEA algorithms were used to assess the immune cell infiltration among the risk score subgroups.ResultsA GRGs-related risk score model was constructed in the TCGA cohort, and validated in the GSE21653 cohort. The risk score was significantly related to the overall survival of breast cancer patients, which could predict the survival prognosis of breast cancer patients independently of other clinical features. Breast cancer patients in the low-risk score group exhibited higher immune cell infiltration levels.ConclusionA novel prognostic model containing five GRGs could accurately predict the survival prognosis and immune infiltration of breast cancer patients. Our findings provided a novel insight into investigating the immunoregulation roles of GRGs.

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“…Of the other variants in genes which showed an up-regulated differential expression between E. coli (+) and E. coli (−) cows, EBF1 and EYA3 are both involved in regulation of gene transcription. GNG7 encodes guanine nucleotide-binding protein γ−7, whose expression level was associated with the infiltration of multiple immune cells into human colorectal cancer samples [ 65 ]. Little information is available concerning FAM129A (also called NIBAN1 ), although expression is up-regulated as part of the integrated stress response [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Circulating Leukocytes Obtained during the Recovery from Clinical Mastitis Caused by Escherichia coli in Holstein Dairy Cows

Cheng

Palma-Vera²,

Buggiotti³

et al. 2022

Animals

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The risk and severity of clinical infection with Escherichia coli as a causative pathogen for bovine mastitis is influenced by the hosts’ phenotypic and genotypic variables. We used RNA-Seq analysis of circulating leukocytes to investigate global transcriptomic profiles and genetic variants from Holstein cows with naturally occurring cases of clinical mastitis, diagnosed using clinical symptoms and milk microbiology. Healthy lactation-matched cows served as controls (CONT, n = 6). Blood samples were collected at two time periods during the recovery phase post diagnosis: EARLY (10.3 ± 1.8 days, n = 6) and LATE (46.7 ± 11 days, n = 3). Differentially expressed genes (DEGs) between the groups were identified using CLC Genomics Workbench V21 and subjected to enrichment analysis. Variant calling was performed following GATKv3.8 best practice. The comparison of E. coli(+) EARLY and CONT cows found the up-regulation of 1090 DEGs, mainly with immune and inflammatory functions. The key signalling pathways involved NOD-like and interleukin-1 receptors and chemokines. Many up-regulated DEGs encoded antimicrobial peptides including cathelicidins, beta-defensins, S100 calcium binding proteins, haptoglobin and lactoferrin. Inflammation had largely resolved in the E. coli(+) LATE group, with only 29 up-regulated DEGs. Both EARLY and LATE cows had up-regulated DEGs encoding ATP binding cassette (ABC) transporters and haemoglobin subunits were also up-regulated in LATE cows. Twelve candidate genetic variants were identified in DEGs between the infected and CONT cows. Three were in contiguous genes WIPI1, ARSG and SLC16A6 on BTA19. Two others (RAC2 and ARHGAP26) encode a Rho-family GTPase and Rho GTPase-activating protein 26. These results show that the initial inflammatory response to E. coli continued for at least 10 days despite prompt treatment and provide preliminary evidence for genetic differences between cows that may predispose them to infection.

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The Prognostic Value of GNG7 in Colorectal Cancer and Its Relationship With Immune Infiltration

Cited by 6 publications

References 48 publications

A Preliminary Investigation of the Differences in Immune Responses in Different Strains of Mice with Localized Allergic Rhinitis Tolerance Models

A Preliminary Investigation of the Differences in Immune Responses in Different Strains of Mice with Localized Allergic Rhinitis Tolerance Models

A novel risk score model based on gamma-aminobutyric acid signature predicts the survival prognosis of patients with breast cancer

Transcriptomic Analysis of Circulating Leukocytes Obtained during the Recovery from Clinical Mastitis Caused by Escherichia coli in Holstein Dairy Cows

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