The prognostic value of long noncoding RNA HOTTIP on clinical outcomes in breast cancer

Abstract

Although a few studies have assessed the prognostic value of long noncoding RNA HOTTIP in patients with malignant tumors, the relationship between HOTTIP and clinical outcome of breast cancer remains elusive. The aim of this study is to explore the prognostic significance of HOTTIP in breast cancer patients. A meta-analysis was performed to involve the eligible studies to investigate the association of HOTTIP expression level with outcome in cancer patients. Pooled hazard ratios (HRs) and 95% confidence interval (CI) of HOTTIP for cancer survival were calculated. Five relevant articles involving 460 patients with various solid carcinomas were included in this meta-analysis. For overall survival, high HOTTIP expression could significantly predict worse outcome with the pooled HR of 2.29 (95 % CI 1.72–3.03, P < 0.00001). Furthermore, Gene Expression Omnibus was performed to evaluate the association of HOTTIP expression with the prognosis in breast cancer patients. It was also found an indication that high HOTTIP expression was associated with worse survival in breast cancer patients by microarray analysis (GSE20711, GSE16446 and GSE9195). Finally, association between HOTTIP levels and clinicopathological factors and prognosis was also analyzed in an independent validation cohort including 100 breast cancer cases. HOTTIP expression was correlated with tumor size (P=0.025), lymph node status (P=0.009) and TNM stage (P=0.0001) in the breast cancer validation cohort. The Kaplan-Meier survival curves indicated that breast cancer patients with high HOTTIP expression had worse overall survival (P=0.0139) and disease-free survival (P=0.0003). Multivariate survival analysis based on the Cox proportional hazards model showed that HOTTP is considered as an independent prognostic factor in breast cancer patients. Together, our combined results suggest that high HOTTIP expression may be serving as an unfavorable prognosis predictor for breast cancer patients.

Comprehensive Analysis of Differentially Expressed Profiles of lncRNAs/mRNAs and miRNAs with Associated ceRNA Networks in Triple-Negative Breast Cancer

“…These data suggest their oncogenic roles in TNBC. Interestingly, our data further support the finding that HOTTIP expression was correlated with tumor size, lymph node status, TNM stage, overall survival and diseasefree survival in the breast cancer [26].…”

“…HOXA11-AS promoted breast cancer invasion and metastasis by regulating epithelial-mesenchymal transition [25]. The expression of HOTTIP was found to be significantly correlated with tumor size, lymph node status, TNM stage and overall survival in the breast cancer patients [26]. Our data further support these findings.…”

Long noncoding RNA HOTTIP mediates SRF expression through sponging miR‐150 in hepatic stellate cells

“…HOTTIP knockdown impedes cell viability, proliferation, invasion, and angiogenesis in human cancer cells, therefore it is been identified as an oncogenic long noncoding RNA (lncRNA). In addition, HOTTIP overexpression is in association with poor prognosis in cancers . HOTTIP expression is regulated by miR‐125, miR‐192, and miR‐204 .…”

Long Non-Coding RNA Emergence During Renal Cell Carcinoma Tumorigenesis

“…A number of lncRNAs have emerged alongside studies related to the biological functions of lncRNAs in human cancers such breast cancer [13], prostate cancer [14], melanoma [15], and RCC [16]. Aberrant lncRNA expression is involved in the recurrence, metastasis, and prognosis of RCC [17][18][19].…”