The Prognostic Value of miR-125b, miR-200c and miR-205 in Primary Cutaneous Malignant Melanoma Is Independent of BRAF Mutational Status

Sánchez‐Sendra, Beatriz; González-Muñoz, José F.; Pérez-Debén, Silvia; Monteagudo, Carlos

doi:10.3390/cancers14061532

Cited by 2 publications

(2 citation statements)

References 45 publications

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“…It is known that the tumor invasion is the cancer progression, associated with the intracellular signaling, transcription and growth factors changes [ 12 , 37 , 65 ] and reflected in the aggressive tumor behavior. In addition, a high hsa-miR-125b expression was associated with the BRAF V600E status, which was disclosed in the results of the experiment on melanoma in cell culture [ 17 ]. The study showed an association between the cancer mutational status and hsa-miR-125b expression.…”

Section: Discussionmentioning

confidence: 99%

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Autophagy-Related MicroRNA: Tumor miR-125b and Thyroid Cancers

Спирина

Kovaleva

Chizhevskaya

et al. 2023

Genes

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Background: Autophagy is a stress response mechanism that causes cellular components to degrade. Its defects were associated with multiple pathologies, including cancers. Thyroid cancer is known to be the most prevalent form of malignant neoplasm among endocrine tumors. The aim of the study was to seek and comprehensively explore the role of autophagy related genes and proteins play in thyroid cancers through bioinformatics analysis with their detection in the tissue samples. Methods: Bioinformatics analysis was performed to investigate autophagy related proteins and genes involvement in thyroid cancer progression. The experimental verification was done in cancer samples of one hundred and three patients with thyroid pathology included in the study. The miR-125blevel was detected by PCR in real time. Results and discussion: The bioinformatics analysis verified the miR-125b as a regulatory mechanism in autophagy. Its expression in patients with PTC was reduced by 6.75 times in cancer patients compared to the patients with benign tumors. The BRAFV600E mutations were associated with a decrease in hsa-miR-125b expression by 12.67 times compared to tumors with the wild-type gene. Conclusions: Our findings revealed involvement of the autophagy related proteins in cancer progression. The significant mechanisms of regulation are non-coding RNA sequences implicated in a variety of oncogenic processes. We found that miR-125b is a potential maker in thyroid cancer invasion, BRAV600E mutational status and risk of recurrence.

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Section: Discussionmentioning

confidence: 99%

“…An association between the miRNA’s rate and the BRAFV600E mutational status was found. It is a significant indicator of the aggressive PTC phenotype [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Autophagy-Related MicroRNA: Tumor miR-125b and Thyroid Cancers

Спирина

Kovaleva

Chizhevskaya

et al. 2023

Genes

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The relationship of clinical and morphological parameters with the BRAF status of the tumor in patients with stage I skin melanoma

Titov,

Sorokina,

Lebedev

et al. 2024

vmirvz

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Aim: to study the relationship between clinical and morphological parameters of skin melanoma and the BRAF status of the tumor in patients with stage I of the disease. Materials and methods. The study was retrospective and included 200 patients with stage I skin melanoma (pT1-2aN0M0), of which BRAF status was assessed in 88 patients. All patients underwent clinical data analysis, an extended morphological study and a molecular genetic study to determine the BRAF V600E mutation in the primary tumor. Results. The median age of patients in the total sample was 61.5 years. Mutation in the BRAF V600E gene was detected in 25 patients (28.4%). Patient age, tumor location and Breslow thickness were recognized as independent predictors of BRAF status of stage I skin melanoma. With an increase in the patient's age by 1 year, the chance of having a BRAF V600E mutation decreased by 3.4% or 1.04 times (OR = 0.966; 95% CI = 0.935–0.999; p = 0.045). When melanoma was localized in the lumbar region, the chance of having a BRAF V600E mutation increased by 15.311 times (95% CI = 1.239–189.142; p = 0.033). With a tumor thickness according to Breslow of more than 0.7 mm, the chance of having a BRAF V600E mutation increased by 2.939 times (95% CI = 1.031-8.376; p = 0.044). With a threshold value of the logistic function of 50%, the sensitivity and specificity of the proposed model were 28.0% and 93.7%, respectively. When the threshold function value is reduced to 25.3%, the sensitivity of the model increases to 68% with a simultaneous drop in specificity to 61.9%. Conclusion. Younger age, greater tumor thickness according to Breslow, and tumor localization in the lumbar region in patients with stage I skin melanoma increase the chance of having a BRAF V600E mutation, while other morphological parameters of the tumor are not associated with BRAF status. However, moderate sensitivity does not allow for a sufficiently accurate determination of the presence of a mutation, thereby strengthening the belief in the need for molecular genetic testing.

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The Prognostic Value of miR-125b, miR-200c and miR-205 in Primary Cutaneous Malignant Melanoma Is Independent of BRAF Mutational Status

Cited by 2 publications

References 45 publications

Autophagy-Related MicroRNA: Tumor miR-125b and Thyroid Cancers

Autophagy-Related MicroRNA: Tumor miR-125b and Thyroid Cancers

The relationship of clinical and morphological parameters with the BRAF status of the tumor in patients with stage I skin melanoma

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