The Prognostic Value of Reticulated Platelets in Patients With Coronary Artery Disease: A Systematic Review and Meta-Analysis

Zhao, Yihan; Peng, Runmin; Zhang, Ying; Shi, Dazhuo

doi:10.3389/fcvm.2020.578041

Cited by 12 publications

(11 citation statements)

References 51 publications

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“…5,11,46 As measuring barbell platelets is based on morphometric evaluation, it has potential to overcome such limitations and could be an invaluable, non-invasive, rapid tool to distinguish peripheral thrombocytopenia from dysfunctional platelet production, 8 accurately defer unnecessary prophylactic platelet transfusions 47 and/or be used as a biomarker/risk factor in antiplatelet therapy and cardiovascular disease, respectively. 48,49 Previous work by Thon and colleagues shows, preplatelet/barbell conversion is dependent on cortical microtubule bundle thickness and diameter. 24 Therefore, it is likely platelet fission is inhibited in giant platelet disorders (e.g.MYH9-RD) where platelets have up to a 20-fold increase in the number of peripheral microtubule coils.…”

Section: Discussionmentioning

confidence: 99%

Analysis of preplatelets and their barbell platelet derivatives by imaging flow cytometry

et al. 2022

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Circulating large "preplatelets" undergo fission via barbell platelet intermediates into two smaller, mature platelets. In this study, we determine whether preplatelets and/or barbells are equivalent to reticulated/immature platelets by using ImageStream flow cytometry (ISFC) and super-resolution microscopy. Immature platelets, preplatelets and barbells were quantified in healthy and thrombocytopenic mice, healthy human volunteers, and patients with immune thrombocytopenia (ITP) or undergoing chemotherapy. Preplatelets and barbells were 1.9%+0.18/1.7%+0.48 (n=6) and 3.3%+1.6/0.5%+0.27 (n=12) of total platelet counts in murine and human whole blood, respectively. Both preplatelets and barbells exhibited high expression of HLA-I with high thiazole orange and mitotracker fluorescence. Tracking dye experiments confirmed that preplatelets transform into barbells and undergo fission ex vivo to increase platelet counts, with dependence upon the cytoskeleton and normal mitochondrial respiration. Samples from antibody-induced thrombocytopenia in mice and patients with ITP had increased levels of both preplatelets and barbells correlating with immature platelet levels. Furthermore, barbells were absent post-chemotherapy in patients. In mice, in vivo biotinylation confirmed that barbells, but not all large platelets, were immature. This study demonstrates that a subpopulation of large platelets are immature preplatelets that can transform into barbells and undergo fission during maturation.

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Section: Discussionmentioning

confidence: 99%

Analysis of preplatelets and their barbell platelet derivatives by imaging flow cytometry

et al. 2022

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“…These platelets have increased volumes and a larger number of dense granules including mRNA. They have increased reactivity, including increased and persistent aggregation formation, compared to mature platelets [ 1 , 12 ]. This increased thrombotic potential has been shown to be of clinical importance, as RP have been associated with adverse cardiac events in multiple settings [ 13 , 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…This increased thrombotic potential has been shown to be of clinical importance, as RP have been associated with adverse cardiac events in multiple settings [ 13 , 14 ]. RP have been suggested as a prognostic marker of adverse cardiovascular events among patients with coronary artery disease [ 1 ]. Indeed, RP are strongly related to residual platelet reactivity despite prasugrel antiplatelet therapy.…”

Section: Discussionmentioning

confidence: 99%

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Reticulated Platelets and Their Relationship with Endothelial Progenitor Cells during the Acute Phase of ST-Elevation Myocardial Infarction

Pravda

Kheifets

Wiessman

et al. 2022

JCM

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Introduction: Endothelial progenitor cells (EPC) and reticulated platelets (RP) have central roles in the thrombotic and angiogenetic interactions during ST-elevation myocardial infarction (STEMI). The EPC and RP response in patients with STEMI treated by primary percutaneous intervention (PPCI) has not yet been investigated. Methods: We assessed EPC quantification by the expression of CD133+ and CD34+, and EPC function by the capacity of the cells to form colony-forming units (CFU) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) during the acute phase of STEMI. These measurements were correlated with RP at baseline and after 24 h following PPCI. Results: Our cohort included 89 consecutive STEMI-diagnosed patients enrolled between December 2018 and July 2021. At baseline, there was a strong positive correlation between reticulated platelet quantity and MTT levels (R = 0.766 and R2 = 0.586, p < 0.001), CD34+ levels (R = 0.602, and R2 = 0.362, p < 0.001); CD133+ levels (R = 0.666 and R2 = 0.443, p < 0.001) and CFU levels (R = 0.437, R2 = 0.191, p < 0.001). The multiple linear regression showed that levels of MTT (adjusted R2 = 0.793; p < 0.001), CD34+ and CD133+ (adjusted R2 = 0.654; p < 0.001 and adjusted R2 = 0.627; p < 0.001, respectively) had strong independent correlations with RP response. At 24 h after PPCI, the correlation between RP quantity and EPC markers was not significant, except for MTT levels (R = 0.465, R2 = 0.216, p < 0.001). Conclusions: In patients with STEMI, higher levels of RP at baseline are significantly correlated with a more potent EPC response. The translational significance of these findings needs further investigation.

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“…The circulating platelet population is heterogeneous in size, age, content, and responsiveness [44]. The latter characteristic has been studied in works focused on assessing the functional and structural differences between subpopulations of platelets concerning the variability of their response according to the different coding and non-coding RNAs within platelets.…”

Section: Plateletsmentioning

confidence: 99%

Platelet Membrane: An Outstanding Factor in Cancer Metastasis

et al. 2022

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In addition to being biological barriers where the internalization or release of biomolecules is decided, cell membranes are contact structures between the interior and exterior of the cell. Here, the processes of cell signaling mediated by receptors, ions, hormones, cytokines, enzymes, growth factors, extracellular matrix (ECM), and vesicles begin. They triggering several responses from the cell membrane that include rearranging its components according to the immediate needs of the cell, for example, in the membrane of platelets, the formation of filopodia and lamellipodia as a tissue repair response. In cancer, the cancer cells must adapt to the new tumor microenvironment (TME) and acquire capacities in the cell membrane to transform their shape, such as in the case of epithelial−mesenchymal transition (EMT) in the metastatic process. The cancer cells must also attract allies in this challenging process, such as platelets, fibroblasts associated with cancer (CAF), stromal cells, adipocytes, and the extracellular matrix itself, which limits tumor growth. The platelets are enucleated cells with fairly interesting growth factors, proangiogenic factors, cytokines, mRNA, and proteins, which support the development of a tumor microenvironment and support the metastatic process. This review will discuss the different actions that platelet membranes and cancer cell membranes carry out during their relationship in the tumor microenvironment and metastasis.

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The Prognostic Value of Reticulated Platelets in Patients With Coronary Artery Disease: A Systematic Review and Meta-Analysis

Cited by 12 publications

References 51 publications

Analysis of preplatelets and their barbell platelet derivatives by imaging flow cytometry

Analysis of preplatelets and their barbell platelet derivatives by imaging flow cytometry

Reticulated Platelets and Their Relationship with Endothelial Progenitor Cells during the Acute Phase of ST-Elevation Myocardial Infarction

Platelet Membrane: An Outstanding Factor in Cancer Metastasis

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