The Proinflammatory Response Induced by Wild-Type<i>Yersinia pseudotuberculosis</i>Infection Inhibits Survival of<i>yop</i>Mutants in the Gastrointestinal Tract and Peyer's Patches

Logsdon, Lauren K.; Mecsas, Joan

doi:10.1128/iai.74.3.1516-1527.2006

Cited by 33 publications

(37 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This clearing effect on yop mutants could even be mediated by inoculating the host with wild-type Y. pseudotuberculosis strains intraperitoneally (i.p.) while challenging the mice with the yop mutants intragastrically (36). While these results suggest that wild-type Y. pseudotuberculosis strains can induce inflammation when injected i.p., we would point out that we use a different species of Yersinia, Y. pestis, and a different route of inoculation (i.v.).…”

Section: Discussioncontrasting

confidence: 45%

“…While these results suggest that wild-type Y. pseudotuberculosis strains can induce inflammation when injected i.p., we would point out that we use a different species of Yersinia, Y. pestis, and a different route of inoculation (i.v.). Furthermore the lack of inflammation by yop mutants seen in the study by Logsdon and Mecsas occurs at the site of the intestine (36). If the yopE and yopH mutants were delivered to deeper tissues, they would likely be rapidly cleared, as has been demonstrated previously for other yop mutants (58).…”

Section: Discussionmentioning

confidence: 54%

“…This is in contrast to some recent findings in the related pathogen Y. pseudotuberculosis. In a recent study, it was shown that yopE and yopH mutants of Y. pseudotuberculosis induce a less dramatic immune response than wild-type Y. pseudotuberculosis during an intragastric infection (36). In fact, the yop mutants were more readily cleared when coinoculated with the wild-type strain, due to increased immune stimulation by the wild-type strain (36).…”

Section: Discussionmentioning

confidence: 99%

“…In a recent study, it was shown that yopE and yopH mutants of Y. pseudotuberculosis induce a less dramatic immune response than wild-type Y. pseudotuberculosis during an intragastric infection (36). In fact, the yop mutants were more readily cleared when coinoculated with the wild-type strain, due to increased immune stimulation by the wild-type strain (36). This clearing effect on yop mutants could even be mediated by inoculating the host with wild-type Y. pseudotuberculosis strains intraperitoneally (i.p.)…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

TheYersinia pestisAil Protein Mediates Binding and Yop Delivery to Host Cells Required for Plague Virulence

2009

View full text Add to dashboard Cite

Although adhesion to host cells is a critical step in the delivery of cytotoxic Yop proteins by Yersinia pestis, the mechanism has not been defined. To identify adhesins critical for Yop delivery, we initiated two transposon mutagenesis screens using the mariner transposon. To avoid redundant cell binding activities, we initiated the screen with a strain deleted for two known adhesins, pH 6 antigen and the autotransporter, YapC, as well as the Caf1 capsule, which is known to obscure some adhesins. The mutants that emerged contained insertions within the ail (attachment and invasion locus) gene of Y. pestis. A reconstructed mutant with a single deletion in the ail locus (y1324) was severely defective for delivery of Yops to HEp-2 human epithelial cells and significantly defective for delivery of Yops to THP-1 human monocytes. Specifically, the Yop delivery defect was apparent when cell rounding and translocation of an ELK-tagged YopE derivative into host cells were monitored. Although the ail mutant showed only a modest decrease in cell binding capacity in vitro, the KIM5 ⌬ail mutant exhibited a >3,000-fold-increased 50% lethal dose in mice. Mice infected with the ⌬ail mutant also had 1,000-fold fewer bacteria in their spleens, livers, and lungs 3 days after infection than did those infected with the parental strain, KIM5. Thus, the Ail protein is critical for both Y. pestis type III secretion in vitro and infection in mice.

show abstract

Section: Discussioncontrasting

confidence: 45%

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

TheYersinia pestisAil Protein Mediates Binding and Yop Delivery to Host Cells Required for Plague Virulence

2009

View full text Add to dashboard Cite

show abstract

“…However, a recent study reported that YopJ-promoted systemic colonization of mice by Y. pseudotuberculosis was associated with higher serum levels of cytokines, including TNF-␣, IFN-␥, and IL-12 (9). In addition, systemic colonization of mice by wild-type Y. pseudotuberculosis was associated with marked signs of inflammation, including elevated levels of Gr1 ϩ CD11b ϩ neutrophils in blood (36). Therefore, it remains unclear to what extent Yersinia, and in particular YopJ, inhibits innate proinflammatory responses during systemic infection.…”

mentioning

confidence: 99%