The proline-rich protein MdPRP6 confers tolerance to salt stress in transgenic apple (Malus domestica)

Zhang, Xiaoli; Gong, Xiaoqing; H, Yu; Su, Xinjian; Cheng, Siyuan; Huang, Jingwen; Lei, Zhaolong; Li, Mingjun; Ma, Fengwang

doi:10.1016/j.scienta.2022.111581

Cited by 10 publications

(7 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, BGLUs may be defense proteins in response to stress, and their upregulation may protect sugar beet from low nitrogen stress. The upregulated POX2 and APX (Supplementary Tables 6, 7) can alleviate low nitrogen stress and improve LN tolerance, which is consistent with previous findings (Hao et al, 2021;Zhang et al, 2023). These results confirm that oxidative damage and compositional changes are sensitive responses for sugar beet to cope with low nitrogen stress.…”

Section: Degs Involved In Conservative Secondary Metabolic Regulatory...supporting

confidence: 91%

Low nitrogen stress-induced transcriptome changes revealed the molecular response and tolerance characteristics in maintaining the C/N balance of sugar beet (Beta vulgaris L.)

Liu

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Nitrogen (N) is an essential macronutrient for plants, acting as a common limiting factor for crop yield. The application of nitrogen fertilizer is related to the sustainable development of both crops and the environment. To further explore the molecular response of sugar beet under low nitrogen (LN) supply, transcriptome analysis was performed on the LN-tolerant germplasm ‘780016B/12 superior’. In total, 580 differentially expressed genes (DEGs) were identified in leaves, and 1,075 DEGs were identified in roots (log2|FC| ≥ 1; q value < 0.05). Gene Ontology (GO), protein−protein interaction (PPI), and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses clarified the role and relationship of DEGs under LN stress. Most of the downregulated DEGs were closely related to “photosynthesis” and the metabolism of “photosynthesis-antenna proteins”, “carbon”, “nitrogen”, and “glutathione”, while the upregulated DEGs were involved in flavonoid and phenylalanine biosynthesis. For example, GLUDB (glutamate dehydrogenase B) was identified as a key downregulated gene, linking carbon, nitrogen, and glutamate metabolism. Thus, low nitrogen-tolerant sugar beet reduced energy expenditure mainly by reducing the synthesis of energy-consuming amino acids, which in turn improved tolerance to low nitrogen stress. The glutathione metabolism biosynthesis pathway was promoted to quench reactive oxygen species (ROS) and protect cells from oxidative damage. The expression levels of nitrogen assimilation and amino acid transport genes, such as NRT2.5 (high-affinity nitrate transporter), NR (nitrate reductase [NADH]), NIR (ferredoxin-nitrite reductase), GS (glutamine synthetase leaf isozyme), GLUDB, GST (glutathione transferase) and GGT3 (glutathione hydrolase 3) at low nitrogen levels play a decisive role in nitrogen utilization and may affect the conversion of the carbon skeleton. DFRA (dihydroflavonol 4-reductase) in roots was negatively correlated with NIR in leaves (coefficient = −0.98, p < 0.05), suggesting that there may be corresponding remote regulation between “flavonoid biosynthesis” and “nitrogen metabolism” in roots and leaves. FBP (fructose 1,6-bisphosphatase) and PGK (phosphoglycerate kinase) were significantly positively correlated (p < 0.001) with Ci (intercellular CO2 concentration). The reliability and reproducibility of the RNA-seq data were further confirmed by real-time fluorescence quantitative PCR (qRT−PCR) validation of 22 genes (R2 = 0.98). This study reveals possible pivotal genes and metabolic pathways for sugar beet adaptation to nitrogen-deficient environments.

show abstract

Section: Degs Involved In Conservative Secondary Metabolic Regulatory...supporting

confidence: 91%

Low nitrogen stress-induced transcriptome changes revealed the molecular response and tolerance characteristics in maintaining the C/N balance of sugar beet (Beta vulgaris L.)

Liu

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…The free proline contributes to the drought stress response in MdPRP6 transgenic apple plants Previously, we found that MdPRP6 affects the free proline accumulation under salt stress (Zhang et al, 2023), and the same physiological changes were also detected under drought stress. The MdPRP6-Ri plants contained higher levels of free proline than WT plants under drought (Figure 8a).…”

Section: Mdrad23d1 Promotes Mdprp6 Degradation Under Drought Stressmentioning

confidence: 74%

“…We found that the cellular free proline is implicated with MdPRP6 ‐mediated drought response (Figure 8a–e ). As previously observed under salt stress (Zhang et al ., 2023 ), we found that the P5CS activity was also higher in MdPRP6 ‐Ri plants under drought (Figure S13a,b ), suggesting that MdPRP6 might affect the P5CS pathway in accumulating proline under stresses. Besides the P5CS pathway, the proline can also be produced from degradation of the PRPs to counteract stress (Barthakur et al ., 2001 ; Gujjar et al ., 2018 , 2019 ; Raymond and Smirnoff, 2002 ; Saikia et al ., 2020 ).…”

Section: Discussionmentioning

confidence: 87%

See 1 more Smart Citation

The ubiquitin‐binding protein MdRAD23D1 mediates drought response by regulating degradation of the proline‐rich protein MdPRP6 in apple (Malus domestica)

Zhang

Gong

et al. 2023

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

Summary RAD23 (RADIATION SENSITIVE23) proteins are a group of UBL‐UBA (ubiquitin‐like‐ubiquitin‐associated) proteins that shuttle ubiquitylated proteins to the 26S proteasome for breakdown. Drought stress is a major environmental constraint that limits plant growth and production, but whether RAD23 proteins are involved in this process is unclear. Here, we demonstrated that a shuttle protein, MdRAD23D1, mediated drought response in apple plants (Malus domestica). MdRAD23D1 levels increased under drought stress, and its suppression resulted in decreased stress tolerance in apple plants. Through in vitro and in vivo assays, we demonstrated that MdRAD23D1 interacted with a proline‐rich protein MdPRP6, resulting in the degradation of MdPRP6 by the 26S proteasome. And MdRAD23D1 accelerated the degradation of MdPRP6 under drought stress. Suppression of MdPRP6 resulted in enhanced drought tolerance in apple plants, mainly because the free proline accumulation is changed. And the free proline is also involved in MdRAD23D1‐mediated drought response. Taken together, these findings demonstrated that MdRAD23D1 and MdPRP6 oppositely regulated drought response. MdRAD23D1 levels increased under drought, accelerating the degradation of MdPRP6. MdPRP6 negatively regulated drought response, probably by regulating proline accumulation. Thus, “MdRAD23D1‐MdPRP6” conferred drought stress tolerance in apple plants.

show abstract

“…Отримані варіанти мають задовольняти наступним умовам: координація підвищеного рівня стрес-стійкості із підвищеним рівнем вільного проліну. Літературні дані вказують на досягнення у отриманні рослин із підвищеним рівнем стійкості до осмотичних стресів із використанням конструкцій, що мають стосунок до метаболізму проліну [14,15,24,27,28,33]. Це стосується і пшениці озимої [3,5,30,32].…”

Section: вступunclassified

Дослідження Характеру Акумуляції Розподілу Вільного Проліну В Органах Рослин За Умов Норми Та Стресу

Броннікова

2023

NCBio

View full text Add to dashboard Cite

Кардинальна зміна кліматичних умов обумовлюють зростаючий дефіцит сільськогосподарських рослин, а також стимулюють розвиток нових біотехнологій. Для отримання форм рослин із підвищеним рівнем стійкості до абіотичних стресів активно використовують методи генетичної інженерії, а саме різні модифікації Agrobacterium-опосередкованої трансформації. В результаті ряду біотехнологічних маніпуляцій були отримані ГМ-рослини Triticum aestivum L. Досліджувались рослини 7-ми добові Т2 рослини пшениці озимої генотипу УК 95/17, та було проаналізовано реакції на дію короткострокових засолення та водного дефіциту, пов’язані з акумуляцією вільного проліну, а також характер відновлення після стресів. Акумуляція протекторної сполуки спрямована на збереження життєздатності культури. Відомо, що пролін за стресових умов може утворюватись як у результаті його підвищеного синтезу, так у наслідок деградації пролін-містких протеїнів клітинної оболонки. Проведені експерименти показали, що трансгенні рослини, відзначаються стійкістю до осмотичного стресу. В той же час тільки паралельне їхнє дослідження може дати більш чітку інформацію про їх характер.

show abstract

The proline-rich protein MdPRP6 confers tolerance to salt stress in transgenic apple (Malus domestica)

Cited by 10 publications

References 66 publications

Low nitrogen stress-induced transcriptome changes revealed the molecular response and tolerance characteristics in maintaining the C/N balance of sugar beet (Beta vulgaris L.)

Low nitrogen stress-induced transcriptome changes revealed the molecular response and tolerance characteristics in maintaining the C/N balance of sugar beet (Beta vulgaris L.)

The ubiquitin‐binding protein MdRAD23D1 mediates drought response by regulating degradation of the proline‐rich protein MdPRP6 in apple (Malus domestica)

Дослідження Характеру Акумуляції Розподілу Вільного Проліну В Органах Рослин За Умов Норми Та Стресу

Contact Info

Product

Resources

About