The promotion of neural progenitor cells proliferation by aligned and randomly oriented collagen nanofibers through β1 integrin/MAPK signaling pathway

Wang, Yansong; Yao, Meng; Zhou, Jingjing; Zheng, Wei; Zhou, Can; Dong, Daming; Liu, Yugang; Teng, Zhaowei; Jiang, Yongqing; Wei, Guojun; Cui, Xiaoying

doi:10.1016/j.biomaterials.2011.05.075

Cited by 79 publications

(65 citation statements)

References 47 publications

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“…11,12 Tissue engineering strategies that incorporate structural anisotropy are crucial in the development of next generation scaffolds fabricated from synthetic materials or natural polymers, such as collagen. 13–17 …”

Section: Introductionmentioning

confidence: 99%

Production of Highly Aligned Collagen Scaffolds by Freeze-drying of Self-assembled, Fibrillar Collagen Gels

Lowe

Reucroft

Grota

et al. 2016

ACS Biomater. Sci. Eng.

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Matrix and cellular alignment are critical factors in the native function of many tissues, including muscle, nerve, and ligaments. Collagen is frequently a component of these aligned tissues, and collagen biomaterials are widely used in tissue engineering applications. However, the generation of aligned collagen scaffolds that maintain the native architecture of collagen fibrils has not been straightforward, with many methods requiring specialized equipment or technical procedures, extensive incubation times, or denaturing of the collagen. Herein, we present a simple, rapid method for fabrication of highly aligned collagen scaffolds. Collagen was assembled to form a fibrillar hydrogel in a cylindrical conduit with high aspect ratio and then frozen and lyophilized. The resulting collagen scaffolds demonstrated highly aligned topographical features along the scaffold surface. This presence of an initial fibrillar network and the high-aspect ratio vessel were both required to generate alignment. The diameter of fabricated scaffolds was found to vary significantly with both the collagen concentration of the hydrogel suspension and the diameter of conduits used for fabrication. Additionally, the size of individual aligned topographical features was significantly dependent on the conduit diameter and the freezing temperature. When cultured on aligned collagen scaffolds, both rat dermal fibroblasts and axons emerging from chick dorsal root ganglia explants demonstrated elongated, aligned morphology and growth on the aligned topographical features. Overall, this method presents a simple means for generating aligned collagen scaffolds that can be applied to a wide variety of tissue types, particularly those where such alignment is critical to native function.

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Section: Introductionmentioning

confidence: 99%

Production of Highly Aligned Collagen Scaffolds by Freeze-drying of Self-assembled, Fibrillar Collagen Gels

Lowe

Reucroft

Grota

et al. 2016

ACS Biomater. Sci. Eng.

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“…5,7,12 It is believed that, as nanofibers resemble an extracellular matrix, at least structurally, they can provide multilayered physical support for stem cell adhesion. 26,27 While this approach has provided dynamic and exciting results for hematopoietic stem cells, 5 neuronal stem cells, 28 and mesenchymal stem calls, 7,29 relatively little is known about how such nanofiber scaffolds enable the recovery and re-ignition of the proliferation of genetically modified human cells. These cells are particularly relevant to nanomedicine because they are the product of ex vivo gene therapy approaches.…”

Section: Introductionmentioning

confidence: 99%

Electrospun fiber membranes enable proliferation of genetically modified cells

Borjigin¹,

Eskridge²,

Niamat³

et al. 2013

IJN

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Polycaprolactone (PCL) and its blended composites (chitosan, gelatin, and lecithin) are well-established biomaterials that can enrich cell growth and enable tissue engineering. However, their application in the recovery and proliferation of genetically modified cells has not been studied. In the study reported here, we fabricated PCL-biomaterial blended fiber membranes, characterized them using physicochemical techniques, and used them as templates for the growth of genetically modified HCT116-19 colon cancer cells. Our data show that the blended polymers are highly miscible and form homogenous electrospun fiber membranes of uniform texture. The aligned PCL nanofibers support robust cell growth, yielding a 2.5-fold higher proliferation rate than cells plated on standard plastic plate surfaces. PCL-lecithin fiber membranes yielded a 2.7-fold higher rate of proliferation, while PCL-chitosan supported a more modest growth rate (1.5-fold higher). Surprisingly, PCL-gelatin did not enhance cell proliferation when compared to the rate of cell growth on plastic surfaces.

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“…( 2011b ) compared the proliferation of neural progenitor cells on aligned and randomly oriented electrospun NFs made of collagen. The authors found that aligned NFs caused faster expansion and a change in the cell cycle progression (Wang et al ., 2011b ). Liu et al .…”

Section: Natural Materialsmentioning

confidence: 96%