The promotion of osteochondral repair by combined intra-articular injection of parathyroid hormone-related protein and implantation of a bi-layer collagen-silk scaffold

Zhang, Wei; Chen, Jialin; Tao, Jiadong; Hu, Caibao; Chen, Longkun; Zhao, Hongxin; Xu, Guangyu; Heng, Boon Chin; Ouyang, Hong

doi:10.1016/j.biomaterials.2013.04.055

Cited by 81 publications

(57 citation statements)

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“…These approaches are not ideal, since they are palliative or induce donor site morbidity. OC tissue engineering emerged as a promising alternative strategy for OCD regeneration [8][9][10][11]. It has been reported that the cartilage cannot spontaneously repair without support from healthy subchondral bone [12].…”

Section: Introductionmentioning

confidence: 99%

Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance

et al. 2015

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Novel porous bilayered scaffolds, fully integrating a silk fibroin (SF) layer and a silk-nano calcium phosphate (silk-nanoCaP) layer for osteochondral defect (OCD) regeneration, were developed. Homogeneous porosity distribution was achieved in the scaffolds, with calcium phosphate phase only retained in the silk-nanoCaP layer. The scaffold presented compressive moduli of 0.4MPa in the wet state. Rabbit bone marrow mesenchymal stromal cells (RBMSCs) were cultured on the scaffolds, and good adhesion and proliferation were observed. The silk-nanoCaP layer showed a higher alkaline phosphatase level than the silk layer in osteogenic conditions. Subcutaneous implantation in rabbits demonstrated weak inflammation. In a rabbit knee critical size OCD model, the scaffolds firmly integrated into the host tissue. Histological and immunohistochemical analysis showed that collagen II positive cartilage and glycosaminoglycan regeneration presented in the silk layer, and de novo bone ingrowths and vessel formation were observed in the silk-nanoCaP layer. These bilayered scaffolds can therefore be promising candidates for OCD regeneration.

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Section: Introductionmentioning

confidence: 99%

Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance

et al. 2015

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“…In addition, natural or synthetic polymers, mentioned earlier, could be used alone or combined with ceramics. 41,43,[88][89][90][91] Ceramics and glasses Ceramics, such as HA or other calcium phosphates, such as tricalcium phosphate (TCP) and bioactive glasses, such as Bioglass Ò , are widely used for bone tissue engineering. [92][93][94][95] These materials promote the formation of a bone-like tissue and enhance integration of the scaffold to the host tissue due to excellent osteoconductivity and osteoinductivity.…”

Section: Materials Of Subchondral Bone Scaffoldmentioning

confidence: 99%

Osteochondral Tissue Engineering with Biphasic Scaffold: Current Strategies and Techniques

Shimomura

Moriguchi²,

Murawski³

et al. 2014

Tissue Engineering Part B: Reviews

113

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The management of osteoarthritis (OA) remains challenging and controversial. Although several clinical options exist for the treatment of OA, regeneration of the damaged articular cartilage has proved difficult due to the limited healing capacity. With the advancements in tissue engineering and cell-based technologies over the past decade, new therapeutic options for patients with osteochondral lesions potentially exist. This review will focus on the feasibility of tissue-engineered biphasic scaffolds, which can mimic the native osteochondral complex, for osteochondral repair and highlight the recent development of these techniques toward tissue regeneration. Moreover, basic anatomy, strategy for osteochondral repair, the design and fabrication methods of scaffolds, as well as the choice of cells, growth factor, and materials will be discussed. Specifically, we focus on the latest preclinical animal studies using large animals and clinical trials with high clinical relevance. In turn, this will facilitate an understanding of the latest trends in osteochondral repair and contribute to the future application of such clinical therapies in patients with OA.

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“…Stromal cell-derived factor-1 (SDF-1) is a key chemokine in cell trafficking and homing of CD34 + stem cells, particularly MSCs [60], and has the potential to enhance cartilage repair through increased MSC migration to the site of a cartilage defect without the need for additional cell transplantation [61, 62]. In the parathyroid hormone (PTH) family, peptide segments of PTH have been shown to inhibit the progression of OA and advance the repair of shallow chondral defects [63, 64]; and PTH-related proteins (PTHrP) are synthesized by chondrocytes and can suppress induction of hypertrophy [65, 66]. However, the timing of PTH or PTHrP administration to cartilage defects remains an important parameter in affecting treatment outcome.…”

Section: Cartilage Regenerative Factorsmentioning

confidence: 99%

Strategies for controlled delivery of biologics for cartilage repair

Lam

Kasper

et al. 2015

Advanced Drug Delivery Reviews

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The delivery of biologics is an important component in the treatment of osteoarthritis and the functional restoration of articular cartilage. Numerous factors have been implicated in the cartilage repair process, but the uncontrolled delivery of these factors may not only reduce their full reparative potential and can also cause unwanted morphological effects. It is therefore imperative to consider the type of biologic to be delivered, the method of delivery, and the temporal as well as spatial presentation of the biologic to achieve the desired effect in cartilage repair. Additionally, the delivery of a single factor may not be sufficient in guiding neo-tissue formation, motivating recent research towards the delivery of multiple factors. This review will discuss the roles of various biologics involved in cartilage repair and the different methods of delivery for appropriate healing responses. A number of spatiotemporal strategies will then be emphasized for the controlled delivery of single and multiple bioactive factors in both in vitro and in vivo cartilage tissue engineering applications.

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The promotion of osteochondral repair by combined intra-articular injection of parathyroid hormone-related protein and implantation of a bi-layer collagen-silk scaffold

Cited by 81 publications

References 50 publications

Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance

Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance

Osteochondral Tissue Engineering with Biphasic Scaffold: Current Strategies and Techniques

Strategies for controlled delivery of biologics for cartilage repair

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