The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast

Kallgren, Scott P.; Andrews, Stuart; Tadeo, Xavier; Hou, Haitong; Moresco, James J.; Tu, Patricia G.; Yates, John R.; Nagy, Péter L.; Jia, Songtao

doi:10.1371/journal.pgen.1004334

Cited by 26 publications

(35 citation statements)

References 53 publications

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“…Interestingly, splicing factors have also been associated with TGS, such as the SR45 protein that is required to establish RdDM and 24-nucleotide siRNA processing at FLOWERING WAGENINGEN (FWA) transgenes 89 . However, Pol IV-dependent transcripts involved in RdDM activity appear to be unspliced 90 , supporting the idea that SR45 participates in RdDM indirectly, perhaps by regulating the splicing of RdDM components, as has been proposed in S. pombe 91–94 .…”

Section: Secondary Sirna Biogenesis and Controlsupporting

confidence: 67%

The expanding world of small RNAs in plants

Borges

Martienssen

2015

Nat Rev Mol Cell Biol

856

774

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Plant genomes produce a variety of small RNAs that function in distinct, yet overlapping, genetic and epigenetic silencing pathways. However, the abundance and diversity of small RNA classes varies in different plant species, suggesting co-evolution between environmental adaptations and gene silencing mechanisms. Small RNA biogenesis in plants is well understood, but we are just beginning to uncover their intricate regulation and activity. Here, we discuss the biogenesis of plant small RNAs, such as microRNAs, secondary small-interfering RNAs and heterochromatic small-interfering RNAs, and their diverse cellular and developmental functions, including reproductive transitions, genomic imprinting and paramutation. We also discuss the diversification of small RNA-directed silencing pathways through the expansion of RNA-dependent RNA polymerases, Dicer and Argonaute proteins.

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Section: Secondary Sirna Biogenesis and Controlsupporting

confidence: 67%

The expanding world of small RNAs in plants

Borges

Martienssen

2015

Nat Rev Mol Cell Biol

856

774

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“…However, earlier biochemical purifications demonstrated that the RDRC subunit Cid12 forms RDRC-independent complexes with splicing factors 27 . Moreover, the RNAi defect found in several splicing mutants can be partially rescued by the introduction of cDNAs that encode RNAi components 45 , suggesting that the effects of splicing mutations on heterochromatic silencing are at least partially due to improper splicing of pre-mRNAs that encode RNAi proteins.…”

Section: Rnai-mediated Heterochromatin Assemblymentioning

confidence: 99%

RNA-mediated epigenetic regulation of gene expression

2015

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Diverse classes of RNA, ranging from small to long non-coding RNAs, have emerged as key regulators of gene expression, genome stability and defence against foreign genetic elements. Small RNAs modify chromatin structure and silence transcription by guiding Argonaute-containing complexes to complementary nascent RNA scaffolds and then mediating the recruitment of histone and DNA methyltransferases. In addition, recent advances suggest that chromatin-associated long non-coding RNA scaffolds also recruit chromatin-modifying complexes independently of small RNAs. These co-transcriptional silencing mechanisms form powerful RNA surveillance systems that detect and silence inappropriate transcription events, and provide a memory of these events via self-reinforcing epigenetic loops.

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“…Due to the effect of C-terminal tags on the functionality of Tpz1 (Kallgren et al 2014), the Tpz1-I501R and Tpz1-I200R used in this study were constructed without any tags unless otherwise noted. All strains containing tpz1Δ and pot1Δ were first constructed in diploid cells, followed by tetrad dissection to obtain desired haploid colonies.…”

Section: Fission Yeast Strains and Genetic Analysesmentioning

confidence: 99%

The proper connection between shelterin components is required for telomeric heterochromatin assembly

et al. 2016

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Telomeric regions contain prominent sites of heterochromatin, which is associated with unique histone modification profiles such as the methylation of histone H3 at Lys9 (H3K9me). In fission yeast, the conserved telomeric shelterin complex recruits the histone H3K9 methyltransferase complex CLRC to establish subtelomeric heterochromatin. Although many shelterin mutations affect subtelomeric heterochromatin assembly, the mechanism remains elusive due to the diverse functions of shelterin. Through affinity purification, we found that shelterin directly associates with CLRC through the Ccq1 subunit. Surprisingly, mutations that disrupt interactions between shelterin subunits compromise subtelomeric heterochromatin without affecting CLRC interaction with shelterin component Pot1, located at chromosome ends. We further discovered that telomeric repeats are refractory to heterochromatin spreading and that artificial restoration of shelterin connections or increased heterochromatin spreading rescued heterochromatin defects in these shelterin mutants. Thus, subtelomeric heterochromatin assembly requires both the recruitment of CLRC by shelterin to chromosome ends and the proper connection of shelterin components, which allows CLRC to skip telomeric repeats to internal regions.

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The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast

Cited by 26 publications

References 53 publications

The expanding world of small RNAs in plants

The expanding world of small RNAs in plants

RNA-mediated epigenetic regulation of gene expression

The proper connection between shelterin components is required for telomeric heterochromatin assembly

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