The Properties of Binding Sites of miR-619-5p, miR-5095, miR-5096, and miR-5585-3p in the mRNAs of Human Genes

Иващенко, А. Т.; Berillo, Olga; Pyrkova, Anna; Niyazova, Raigul; Atambayeva, Shara

doi:10.1155/2014/720715

Cited by 21 publications

(24 citation statements)

References 19 publications

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“…Understanding their individual and collective roles is important when studying the development of this neoplasia. miR-5095 had the highest number of binding sites distributed throughout the human genome (Table 3), which is in accordance with previously reported data [66][67][68] where approximately 900 binding sites were identified; they are probably related to the expression of many target mRNAs and biological processes. Based on its extensive genomic distribution and low specificity in CC, miR-5095 is a good candidate to be used as an indicator of genetic variability within the human population.…”

Section: Mirna Binding Sites Associated With Cervical Cancersupporting

confidence: 92%

Mapping of microRNAs related to cervical cancer in Latin American human genomic variants

et al. 2017

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MicroRNAs are related to human cancers, including cervical Background cancer (CC), which is mainly caused by human papillomavirus (HPV) infection. In 2012, approximately 70000 cases and 28000 deaths from this cancer were registered in Latin America according to GLOBOCAN reports. The most frequent genotype worldwide is HPV-16. The main molecular mechanism of HPV in CC is related to integration of viral DNA into the hosts' genome. However, the different variants in the human genome can result in different integration mechanisms, specifically involving microRNAs (miRNAs).: miRNA sequences associated with CC and four human genome Methods variants from Latin American populations were obtained from miRBase and the 1000 Genomes Browser, respectively. HPV integration sites near cell cycle regulatory genes were identified. miRNAs were mapped on human genomic variants. miRSNPs (single nucleotide polymorphisms in miRNAs) were identified in the miRNA sequences located at HPV integration sites on the human genomic Latin American variants.: Two hundred seventy-two miRNAs associated with CC were Results identified in 139 reports from different geographic locations. By mapping with the Blast-Like Alignment Tool (BLAT), 2028 binding sites were identified from these miRNAs on the human genome (version GRCh38/hg38); 42 miRNAs were located on unique integration sites; and miR-5095, miR-548c-5p and miR-548d-5p were involved with multiple genes related to the cell cycle. Thirty-seven miRNAs were mapped on the human Latin American genomic variants (PUR, MXL, CLM and PEL), but only miR-11-3p, miR-31-3p, miR-107, miR-133a-3p, miR-133a-5p, miR-133b, miR-215-5p, miR-491-3p, miR-548d-5p and miR-944 were conserved.: 10 miRNAs were conserved in the four human genome variants, Conclusions and in the remaining 27 miRNAs, substitutions, deletions or insertions were observed in the nucleotide sequences. This variability can imply differentiated mechanisms towards each genomic variant in human populations, relative to specific genomic patterns and geographic features. These findings may be decisive in determining susceptibility to the development of CC. Further identification of cellular genes and signalling pathways involved in CC progression could lead to the development of new therapeutic strategies based on miRNAs.

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Section: Mirna Binding Sites Associated With Cervical Cancersupporting

confidence: 92%

Mapping of microRNAs related to cervical cancer in Latin American human genomic variants

et al. 2017

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“…miR-619-5p is a miRNA that binds with high affinity to the mRNAs of 1,215 genes, and miR-619-5p has binding sites in the coding sequences and UTRs of mRNAs (19). Bioinformatics analysis indicated that miR-619-5p has several binding sites on the 3'-UTR of MALAT1.…”

Section: Discussionmentioning

confidence: 99%

“…Other studies have described the altered expression of miRNAs in cancer tissues compared with normal tissues (17,18), suggesting that these miRNAs may potentially indicate novel clinical diagnostic and prognostic markers. miRNA-619-5p (miR-619-5p) is a miRNA that binds with high affinity to the messenger (m)RNA of 1,215 genes, and miR-619-5p has binding sites in the coding sequences and untranslated regions (UTRs) of mRNAs (19).…”

Section: Introductionmentioning

confidence: 99%

Dysregulation of MALAT1 and miR-619-5p as a prognostic indicator in advanced colorectal carcinoma

Zhang

et al. 2016

Oncology Letters

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Abstract. The present study aimed to detect the expression of metastasis associated lung adenocarcinoma transcript 1 (MALAT1) and microRNA (miR)-619-5p in colorectal carcinoma (CRC), and to evaluate the significance of MALAT1 and miR-619-5p expression in the clinical diagnosis and prognosis of CRC. Quantitative polymerase chain reaction was used to detect MALAT1 and miR-619-5p expression in 120 colorectal carcinoma and 120 adjacent normal tissue samples. The expression levels of MALAT1 and miR-619-5p were significantly different between colorectal carcinoma and adjacent normal tissues (P<0.05). MALAT1 exhibited an average 2.52-fold increase in colorectal adenoma when compared with adjacent normal tissues, while miR-619-5p exhibited an average 5.79-fold decrease in colorectal adenoma when compared with adjacent normal tissues. There was a significant difference between the MALAT1 expression in CRC tissues obtained from men and women (P=0.027), and in tumor-node-metastasis (TNM) stage II and stage III lesions (P=0.019). MALAT1 expression was associated with lymphovascular invasion (P=0.047) and perineural invasion (P=0.012). In addition, miR-619-5p expression was also significantly different between men and women (P=0.032), and between TNM stage II and stage III lesions (P=0.012). miR-619-5p expression was also associated with lymphovascular invasion (P=0.023) and perineural invasion (P=0.009). Patients with high expression of MALAT1 and low expression of miR-619-5p demonstrated significantly shorter disease-free survival (DFS) (P=0.002) and overall survival (OS) times (P=0.004) compared with patients with low MALAT1 expression and high miR-619-5p expression. Patients with perineural invasion demonstrated significantly shorter DFS (P=0.001) and OS times (P=0.003) compared with patients without perineural invasion. In addition, there was a negative correlation between MALAT1 expression and miR-619-5p expression (r=-0.415, P=0.004) in CRC tissues. In conclusion, MALAT1 and miR-619-5p have potential for the molecular diagnosis of CRC patients, and combined assaying of MALAT1 and miR-619-5p may improve the accuracy of the diagnosis of CRC and act as a good prognostic indicator in CRC patients.

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“…Ранее было показано, что сайты связывания miR-5096 локализуются на 832 таргетных матричных РНК [13]. Возможно, такое достаточно большое количество сайтов связывания говорит нам о функциональной роли miR-5096 в качестве координатора экспрессии больших генных ансамблей.…”

Section: Serpine1unclassified

Search for binding sites for micro RNA in cisregulatory sequences and in SNP in the lipid, carbohydrate metabolism, oxidative and antiinflammatory homeostasis genes

Neskubina¹,

Amelina²,

Шкурат³

et al. 2019

RR in B

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Background: Polymorphisms and disruption of the expression profile of miRNA genes are associated with systemic diseases (autoimmune and cardiovascular diseases). The aim of the study: To study the localization of miRNA binding sites to mRNA in cis-regulatory regions of genes and in the coding sequences of DNA (CDS) associated in our studies with early and late atherosclerosis, and to search for possible localization of binding sites to microRNAs with gene sites (PPARGC1A; LIPC; PON1; APOE; LPL; APOC3; EDN; TNFα; SERPINE1). Materials and methods: The search for homologous micro RNA motifs was carried out in the cisregulatory regions of the studied genes with the help of the bio-information package MEME Suite. Known miRNAs were taken from the miRBase database (http://mirbase.org/). Nucleotide sequences of cis-regulatory regions and gene introns were obtained from the NCBI database (http://www.ncbi.nlm.nih.gov/) using a set of scripts, IFITCH, designed to automatically retrieve data from NCBI sequences. The "miRBase" database was analyzed using automated search for binding sites in the original sequence using the MScanner classifier. 28645 microRNAs were registered (http://www.mirbase.org). Results: The results of the bioinformational search for the localization of motifs homologous to known microRNAs before and after the gene, as well as in the coding protein sequences and introns of the following genes: PON1, APOC3, LIPC, LPL, APOE, PPARGC1A, TNF, EDN, SERPIN showed that in genes and intergenic spaces there are a large number of motifs homologous to mature micro-RNA. The greatest absolute number of motifs is localized within the PPARGC1A gene-22 microRNAs. However, if we consider the relative frequency

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The Properties of Binding Sites of miR-619-5p, miR-5095, miR-5096, and miR-5585-3p in the mRNAs of Human Genes

Cited by 21 publications

References 19 publications

Mapping of microRNAs related to cervical cancer in Latin American human genomic variants

Mapping of microRNAs related to cervical cancer in Latin American human genomic variants

Dysregulation of MALAT1 and miR-619-5p as a prognostic indicator in advanced colorectal carcinoma

Search for binding sites for micro RNA in cisregulatory sequences and in SNP in the lipid, carbohydrate metabolism, oxidative and antiinflammatory homeostasis genes

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