The prospects of using Bacillus amyloliquefaciens in the biological control of grape diseases

Abstract

Currently, the prospects of effective control management in the development of gray (Botrytis cinerea Pers.) and black rot (Macrophoma flaccida (Viala et Rav.) Cav.), as well as the guaranteed preservation of grape yield, can be achieved with the development of ecological approach - the use of microbiological preparations or substances of biological origin. The antagonistic activity of biological fungicide Serenade ASO, SC (titer not less than 1x109 CFU/ml of Bacillus amyloliquefaciens, strain QST 713) was studied in the field research vineyards of the South-Western (SWC) and South Coastal (SCC) viticulture zones of Crimea in 2018-2019. Against the background of weak development of gray rot on bunches of ‘Cabernet-Sauvignon’ grapes (SCC), the maximum biological efficiency was obtained in the variant with five-fold spraying of Serenade ASO, SC, application rate of 5 l/ha and seven days interval. Two-fold protective use of biological preparation Serenade ASO, SC and application rate of 5 l/ha on the plot of ‘Muscat Blanc’ variety (SCC) in 2020 made it possible to control the development of black rot at the level of 76.7-79%. The obtained results indicate good prospects of biological preparation Serenade ASO, SC (titer not less than 1x109 CFU/ml of Bacillus amyloliquefaciens, strain QST 713) to control gray and black rot of grapes during ripening, when the use of chemical fungicides is not appropriate due to their lagged effect.

Insights into the Interaction between the Biocontrol Agent Bacillus amyloliquefaciens QST 713, the Pathogen Monilinia fructicola and Peach Fruit

“…and biological control of brown rot disease, with B. amyloliquefaciens and B. subtilis being the most promising species [33,34]. Among them, B. amyloliquefaciens QST 713 (formerly B. subtilis QST 713) is a successful commercialized BCA product capable of suppressing the growth of numerous fungal pathogens and effectively controlling postharvest diseases, including gray mold in grapes and strawberries caused by the necrotrophic fungus B. cinerea, black rot in grapes caused by Macrophoma flaccida and brown rot in peaches caused by M. fructicola [19,24,33,[35][36][37]. In our study, we investigated the effect of B. amyloliquefaciens QST 713 on the biological control of brown rot in peach fruits, the direct in vitro antagonistic activity of this BCA against M. fructicola, the defense-related physiological indices and the transcriptional responses of fruits during the confrontation of B. amyloliquefaciens QST 713 with M. fructicola.…”