The proteasomes of two marine decapod crustaceans, European lobster (Homarus gammarus) and Edible crab (Cancer pagurus), are differently impaired by heavy metals

Abstract

The intracellular ubiquitin-proteasome system is a key regulator of cellular processes involved in the controlled degradation of short-living or malfunctioning proteins. Certain diseases and cellular dysfunctions are known to arise from the disruption of proteasome pathways. Trace metals are recognized stressors of the proteasome system in vertebrates and plants, but their effects on the proteasome of invertebrates are not well understood. Since marine invertebrates, and particularly benthic crustaceans, can be exposed to high metal levels, we studied the effects of in vitro exposure to Hg 2+ , Zn 2+ , Cu 2+ , and Cd 2+ on the activities of the proteasome from the claw muscles of lobsters (Homarus gammarus) and crabs (Cancer pagurus). The chymotrypsin like activity of the proteasome of these two species showed different sensitivity to metals. In lobsters the activity was significantly inhibited by all metals to a similar extent. In crabs the activities were severely suppressed only by Hg 2+ and Cu 2+ while Zn 2+ had only a moderate effect and Cd 2+ caused almost no inhibition of the crab proteasome. This indicates that the proteasomes of both species possess structural characteristics that determine different susceptibility to metals. Consequently, the proteasome-mediated protein degradation in crab C. pagurus may be less affected by metal pollution than that of the lobster H. gammarus.

The distribution of heavy metals content in the bottom deposits of the trans-border Uzh river system

“…Нині одна з основних екологічних проблемзабруднення поверхневих вод (Islam еt al., 2015;Mohiuddin, et al, 2016). Інтенсифікація антропогенного навантаження в сукупності з природно-кліматичними факторами викликає значні зміни у структурі гідроекосистем, які супроводжуються порушенням гідрологічного та гідрохімічного режимів, біотрансформацією екотоксикантів та їх акумуляцією в ланцюгах живлення, кінцева ланка якихлюдина та тварини (Bo et al, 2014;Götze et al, 2014;Brygadyrenko and Ivanyshyn, 2015;Kul'bachko et al, 2015;Tsvetkova et al, 2016). До одних із найнебезпечніших забруднювачів водних об'єктів належать важкі метали (Fujita et al, 2014;Shakeri et al, 2016).…”

Determine heavy metals in water, aquatic plants, and sediment in water systems

“…Sediments are good absorbers of heavy metals, and might be also a source of food for benthic organisms. The accumulation of heavy metals in certain conditions leads to environmental damage, moreover might cause a threat to human health through accumulation in the food chain [3]. Zn and Cu are very special trace elements because of their double function in plants.…”

Trace Metal Contamination Characteristics and Health Risks Assessment ofCommelina africanaL. and Psammitic Sandflats in the Niger Delta, Nigeria

“…Also, they could be released in both particulate and dissolved forms and are known to have high affinities for fine-grained sediment and soil particulates [24][25][26][27]. However, the fate, transport, and pollution characteristics of trace metals in the wetland soils have become an important problem due to their toxic effects, accumulation, and bioconcentration through the food chain [28,29].…”

“…Adverse health effects, such as lung and skin cancer, prostatic proliferative lesions, peripheral neuropathy, kidney dysfunction, dermal lesions, and peripheral vascular disease, have been attributed to trace metals pollution. However, metal toxicity mainly depends on the metal speciation and bioavailability, as well as on the means of uptake, accumulation, and excretion rates of the organisms [24,28,[33][34][35]. Therefore, elucidating the potential sources, ionic forms, ecosystem variability, pollution status, and environmental risks, assessment of trace metals in wetland soil environment is a critical tool in understanding the contamination characteristics of such ecosystems.…”