The Protection of Salidroside of the Heart against Acute Exhaustive Injury and Molecular Mechanism in Rat

Abstract

Objective. To investigate the protection of salidroside of the heart against acute exhaustive injury and its mechanism of antioxidative stress and MAPKs signal transduction. Method. Adult male SD rats were divided into four groups randomly. Cardiomyocytes ultrastructure was observed by optical microscopy and transmission electron microscopy. The contents of CK, CK-MB, LDH, MDA, and SOD were determined by ELISA method, and the phosphorylation degrees of ERK and p38 MAPK were assayed by Western blotting. Cardiac function of isolated rat heart ischemia/reperfusion was detected by Langendorff technique. Results. Salidroside reduced the myocardium ultrastructure injury caused by exhaustive swimming, decreased the contents of CK, CK-MB, and LDH, improved the LVDP, ±LV dp/dt max under the basic condition, reduced the content of MDA and the phosphorylation degree of p38 MAPK, and increased the content of SOD and the phosphorylation degree of ERK in acute exhaustive rats. Conclusion. Salidroside has the protection of the heart against acute exhaustive injury. The cardioprotection is mainly mediated by antioxidative stress and MAPKs signal transduction through reducing the content of MDA, increasing the content of SOD, and increasing p-ERK and decreasing p-p38 protein expressions in rat myocardium, which might be the mechanisms of the cardioprotective effect of salidroside.

Rhodiola crenulata extract counteracts the effect of hypobaric hypoxia in rat heart via redirection of the nitric oxide and arginase 1 pathway

“…These results indicated that RCE promotes cardiomyocyte survival under acute hypobaric hypoxia exposure by p38MAPK, but not ERK, signaling pathway in heart tissue. These results are similar to those observed in the acute exhaustive rodent model where salidroside regulates MAPK signaling pathways, through modification of ROS generation [15]. Thus, the regulatory effect of RCE on MAPK pathways might again be due to the antioxidant property of RCE.…”

“…In a rodent model of exhaustive exercise, salidroside, a major bioactive compound of RCE, exerted a cardioprotective effect via its antioxidant activity [15]. In this study, we have shown that RCE reduces biomarkers of cardiac oxidative stress (ROS, MDA, and protein carbonyl) in rat myocardium.…”

“…The heart is very sensitive to hypoxia due to its property of high oxygen uptake [15]. Exposure to hypobaric hypoxia can consequently result in exaggerated arterial hypoxemia and lead to cardiac dysfunction including SCD [2, 19].…”

“…Rhodiola crenulata , a popular folk medicine for anti-altitude sickness in Tibet [12], has been recently reported as having cardio-protective effects on hypoxia in vivo [13, 14] and in rodent models of acute exhaustive injury [15]. However, the detailed mechanisms by which RCE protects against hypoxia remain unclear.…”

Effect of Salvianolic Acid B on Cardiac Muscle Damage Following Exhaustive Exercise in Rats

“…한편으로 급격하고 과도한 운동은 심근세포의 산소 소모 를 증가시키므로 활성산소종(reactive oxygen species, ROS) 생성을 증가시키고 심장의 구조적, 기능적 및 대사적 장애를 초래한다 16,17) . Fig.…”