The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats

Abstract

Cadmium (Cd) is an environmental carcinogenic pollutant known to inactivate several proteins involved in DNA repair systems while at the same time creating an oxidative stress that can result in additional DNA lesions. The testis and the lung are the target organs for cadmium carcinogenesis. Increased production of oxidants in vivo can cause damage to intracellular macromolecules such as DNA, proteins and lipids, which in turn lead to oxidative injury. So, this investigation aimed to evaluate the protective role of L-Carnitine through up regulation of HSPs against DNA damage induced by cadmium chloride. The current study was carried out on forty adult male rats, each with average weight 220-250g., were divided into 4 equal groups. 1st group was received saline solution (0.5 ml/100 g body weight) and kept as control. 2nd group was received 500mg / kg body weight L-Carnitine intraperitoneally (IP). 3rd group was administered 1.2 mg cadmium chloride IP. 4th group was received both cadmium chloride and L-Carnitine simultaneously. The comet assay parameters showed significantly increased HSP70 and DNA damage in testis cells after 10 and 56 days in the third group. Meanwhile, HSP70 showed significantly decreased levels after 10 days and 56 days in the fourth group after L-Carnitine treatment simultaneously with cadmium chloride. The results of the present study demonstrate a damaging effect of cadmium chloride on DNA of the testis cells (with low stress response). This damaging effect increases the synthesis of HSP70 that upregulated by L-Carnitine treatment and showed ameliorative effect of the cells for recovery.

Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

“…If the expressions of heat-shock protein (Hsp)/heat-shock factors were changed, abnormal germ cell apoptosis is investigated, resulting in impaired spermatogenesis (Ji et al, 2012). Especially, Hsp70 expression was increased in the rat myocardium of DM animals (Ugurlucan et al, 2010) and in cadmium-exposed testicular tissue (Selim et al, 2012). The present study also revealed the increase of testicular Hsp70 in T2DM rats, which is consistent with previous reports demonstrated in DM animals (Selim et al, 2012;Ugurlucan et al, 2010).…”

<p>Piceatannol inhibits oxidative stress through modification of Nrf2-signaling pathway in testes and attenuates spermatogenesis and steroidogenesis in rats exposed to cadmium during adulthood</p>

“…The decrease in testis weight may be an indication of generation of free radicals. Free radicals cause oxidation and thus deterioration in DNA, proteins, and lipids, which leads to testicular atrophy 30,31. Histological evaluation of testes revealed severe effects in the form of degenerative changes in seminiferous tubules with loss of spermatogenesis after treatment of rats with Cd.…”

Heat shock protein 70 protects rat peritoneal mesothelial cells from advanced glycation end-products-induced epithelial-to-mesenchymal transition through mitogen-activated protein kinases/extracellular signal-regulated kinases and transforming growth factor-β/Smad pathways

“…The HSP70 protein family and their co-chaperones constitute a complex network of folding machines which is utilized by cells in numerous ways, allowing them to adapt to gradual changes in their environment and to survive in what would otherwise be lethal conditions (23). With respect to the HSP70 proteins it is elusive whether their activity inhibits EMT in RPMCs.…”