The Protective Activity of Tea Catechins against Experimental Infection by <i>Vibrio cholerae</i> O1

Toda, Masako; Okubo, Sachie; Ikigai, Hajime; Suzuki, Tatsuo; Suzuki, Yumiko; Hara, Yukihiko; Shimamura, Tadakatsu

doi:10.1111/j.1348-0421.1992.tb02103.x

Cited by 93 publications

(56 citation statements)

References 10 publications

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“…Polyphenols that inhibit CT-induced fluid accumulation may exert an additional anti-diarrheal effect. For this purpose, other investigators have reported that tea polyphenol has an inhibitory effect on CT-induced fluid accumulation in sealed adult mice and the ligated intestinal loops of rabbits (19) and that it has a protective effect on gnotobiotic mice infected with the Escherichia coli O157:H7 strain (8). Similar to tea polyphenols, APE is frequently used as a food additive.…”

Section: Discussionmentioning

confidence: 99%

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Inhibition by Apple Polyphenols of ADP‐Ribosyltransferase Activity of Cholera Toxin and Toxin‐Induced Fluid Accumulation in Mice

Saito

Miyake

Toba

et al. 2002

Microbiology and Immunology

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The effects of crude polyphenol extracted from immature apples on the enzymatic and biological activities of a cholera toxin (CT) were investigated. When the apple polyphenol extract (APE) was examined for properties to inhibit CT‐catalyzed ADP‐ribosylation of agmatine, it was found that APE inhibited it in a dose‐dependent manner. The concentration of APE to inhibit 50% of the enzymatic activity of CT (15 μg/ml) was approximately 8.7 μg/ml. The APE also diminished CT‐induced fluid accumulation in two diarrhea models for in vivo mice. In the ligated ileum loops, 25 μg of APE significantly inhibited fluid accumulation induced by 500 ng of CT. In a sealed mouse model, even when APE was administered orally 10 min after a toxin injection, fluid accumulation was significantly inhibited at a comparable dosage. Lineweaver‐Burk analysis demonstrated that APE had negative allosteric effects on CT‐catalyzed NAD: agmatine ADP‐ribosyltransferase. We fractionated the APE into four fractions using LH‐20 Sephadex resin. One of the fractions, FAP (fraction from apple polyphenol) 1, which contains non‐catechin polyphenols, did not significantly inhibit the CT‐catalyzed ADP‐ribosylation of agmatine. FAP2, which contains compounds with monomeric, dimeric, and trimeric catechins, inhibited the ADP‐ribosylation only partially, but significantly. FAP3 and FAP4, which consist of highly polymerized catechin compounds, strongly inhibited the ADP‐ribosylation, indicating that the polymerized structure of catechin is responsible for the inhibitory effect that resides in APE. The results suggest that polymerized catechin compounds in APE inhibit the biological and enzymatic activities of CT and can be used in a precautionary and therapeutic manner in the treatment of cholera patients.

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Section: Discussionmentioning

confidence: 99%

“…Toda et al (19) have reported that tea polyphenol inhibited the fluid accumulation induced by CT in sealed adult mice. Effects of other polyphenols, however, were not elucidated until recently.…”

mentioning

confidence: 99%

Inhibition by Apple Polyphenols of ADP‐Ribosyltransferase Activity of Cholera Toxin and Toxin‐Induced Fluid Accumulation in Mice

Saito

Miyake

Toba

et al. 2002

Microbiology and Immunology

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“…In microbiological terms, much of the postulated benefit to be derived from tea drinking is anecdotal (e.g., see reference 33). Toda et al (43) found that a mixture of tea catechins protected rabbits from an experimental infection caused by V. cholerae and suggest that patients with cholera could benefit if tea extracts were added to oral rehydration solutions.…”

Section: Biological Activities Of Tea Componentsmentioning

confidence: 99%

Antimicrobial properties of tea (Camellia sinensis L.)

Hamilton‐Miller

1995

Antimicrob Agents Chemother

318

168

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“…Toda et al (6)(7)(8)(9) (12) concluyeron que la Candida albicans no era sensible a la acción inhibitoria del té. Los extractos de té además, son capaces de prevenir las infecciones por rotavirus y enterovirus (13) lo cual no se debería a una acción antiviral directa sino a una adsorción del virus.…”

Section: Introductionunclassified

Efectividad del té verde en el tratamiento de periodontitis crónica

et al. 2005

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Funosas ER, Martínez AB, Pignolo M, Maestri L, Aromando RF, Scozzarro SM, Escovich L, Hermida PS Efectividad del té verde en el tratamiento de periodontitis crónica AVANCES EN ODONTOESTOMATOLOGÍA/159Efectividad del té verde en el tratamiento de periodontitis crónica Funosas ER, Martínez AB, Pignolo M, Maestri L, Aromando RF, Scozzarro SM, Escovich L, Hermida PS RESUMENEl té verde ha mostrado un amplio rango de efectos fisiológicos y farmacológicos. En los últimos años se han llevado a cabo estudios sistemáticos principalmente por investigadores japoneses en los cuales se evidenciaron las acciones antimicrobianas útiles y variadas que poseen los extractos de té. El objetivo de este trabajo fue determinar, mediante el uso de registros cuantificables, la efectividad clínica y microbiológica del té verde en el tratamiento de las periodontitis crónicas. Fueron evaluados 50 pacientes de ambos sexos, con diagnóstico de periodontitis crónica y con un mínimo de tres bolsas periodontales por cuadrante con profundidad de sondaje ≥ 5 mm y pérdida de inserción ≥ 2 mm por proximal, que concurrieron a la Cátedra de Periodoncia de la Facultad de Odontología de la Universidad Nacional de Rosario, Argentina. Las variables clínicas consideradas fueron Indice Gingival (Loe y Silness, 1963), Sangrado al Sondaje (Val del Verden, 1979), Profundidad de sondaje con sonda milimetrada tipo Marquis y Nivel de inserción epitelial vertical. Para determinar la eficacia microbiológica del tratamiento se extrajeron muestras de placa subgingival con conos de papel estéril del fondo de las bolsas periodontales. Se sembró en medio de Agar Schlaeder -sangre enriquecido con 1% de hemina y vitamina K en atmós-fera anaeróbica a 37°C durante 5 días. La tipificación fue realizada con el método comercial semi-automatizado Api 20 A Biomerieux -France. El uso de extracto de té verde asociado a la terapia periodontal mecánica ha sido sensiblemente eficaz en la reversión de estas variables en el tratamiento de la periodontitis crónica, si bien su uso asociado o no al raspaje y alisamiento radicular no mostró diferencias significativas respecto del raspaje y alisamiento como monoterapia para el control de la flora anaeróbica en esta patología.Palabras clave: te verde -periodontitis crónica-terapia periodontal ABSTRACTGreen tea has been shown to have a wide range of physiological and pharmacological effects. In recent years, systematic studies performed mainly by Japanese researchers have evidenced the wide range of very useful antimicrobial properties of tea extracts. The aim of the present study was to quantitatively evaluate the clinical and microbiological efficacy of green tea in the treatment of chronic periodontitis. Fifty male and female patients who attended the Department of Periodontics, Faculty of Dentistry, National University of Rosario, Argentina, and had been diagnosed with chronic periodontitis were included in the study. The selected patients had at least 3 periodontal pockets per quadrant, depth on probing ≥ 5 mm and proximal attachment loss...

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The Protective Activity of Tea Catechins against Experimental Infection by Vibrio cholerae O1

Cited by 93 publications

References 10 publications

Inhibition by Apple Polyphenols of ADP‐Ribosyltransferase Activity of Cholera Toxin and Toxin‐Induced Fluid Accumulation in Mice

Inhibition by Apple Polyphenols of ADP‐Ribosyltransferase Activity of Cholera Toxin and Toxin‐Induced Fluid Accumulation in Mice

Antimicrobial properties of tea (Camellia sinensis L.)

Efectividad del té verde en el tratamiento de periodontitis crónica

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