The protective activity of α-hederine against H2O2 genotoxicity in HepG2 cells by alkaline comet assay

Gachou, Clémence Mba; Laget, M.; Guiraud-Dauriac, H; Méo, Michel De; Élias, Riad; Duménil, Gérard

doi:10.1016/s1383-5718(99)00117-5

Cited by 17 publications

(7 citation statements)

References 28 publications

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“…PD‐treated cells (10 μmol/L) exhibit the typical morphological features of apoptotic cells, including apoptotic body and the formation of typical DNA ladders in human hepatoma SK‐HEP‐1 cells 30,31 . The α‐hederine monodesmosidic triterpenoid saponin has antimutagenic effects, but the concentration of 30 μg/mL was not sufficient to protect H 2 O 2 ‐induced HepG2 cell damage 32 . Others have reported that Rh 2 , a PD type of ginseng saponin, potently suppresses cell growth of Lewis lung, Morris hepatoma, B16, and HeLa cells 33,34 .…”

Section: Resultsmentioning

confidence: 99%

“…30,31 The α-hederine monodesmosidic triterpenoid saponin has antimutagenic effects, but the concentration of 30 μg/mL was not sufficient to protect H 2 O 2 -induced HepG2 cell damage. 32 Others have reported that Rh 2 , a PD type of ginseng saponin, potently suppresses cell growth of Lewis lung, Morris hepatoma, B16, and HeLa cells. 33,34 Novel ginseng saponin metabolites, such as IH 901, compound K(MI), were formed from the PD series-Rb1, Rb2, and Rc-by human intestinal bacteria deglycosylation.…”

Section: Cell Viabilitymentioning

confidence: 99%

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Antioxidant and Apoptotic Effects of Korean White Ginseng Extracted with the Same Ratio of Protopanaxadiol and Protopanaxatriol Saponins in Human Hepatoma HepG2 Cells

Lee

Sorn

Baek

et al. 2009

Annals of the New York Academy of Sciences

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To investigate the antioxidative effects of ginsenosides [protopanaxadiol derivatives (PD):protopanaxatriol derivatives (PT) = 1:1] from the roots of Korean ginseng, cell viability, malondialdehyde (MDA) production, antioxidant enzyme activities, and expressions of apoptosis were analyzed after pretreatment of human hepatoma HepG2 cells with H(2)O(2). Cell death was increased through H(2)O(2) treatment dose dependently, and a dose of ginseng extract (PD:PT = 1:1) of 18.6 microg/mL was enough to derive it in reverse. MDA production was reduced through the administration of ginseng extracts even with more intensive H(2)O(2) treatments. Through the use of even low levels of ginseng extract (e.g., 1.86 microg/mL), catalase (CAT) activity was easily reduced from the plateau induced by H(2)O(2). The glutathione peroxidase activity was no better than that of CAT. We assume that ginseng extract acts as an antioxidant even when effective levels of ginseng differ. A ginseng extract dose of 18.6 microg/mL increased the apoptotic expression of oxidative stressed signals, such as c-Jun-N-terminal kinase and stress-activated protein kinase expressions, and mitochondrial cytochrome c released caspase-3 activation; however, these expressions changed with higher doses of ginseng.

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Section: Resultsmentioning

confidence: 99%

Section: Cell Viabilitymentioning

confidence: 99%

Antioxidant and Apoptotic Effects of Korean White Ginseng Extracted with the Same Ratio of Protopanaxadiol and Protopanaxatriol Saponins in Human Hepatoma HepG2 Cells

Lee

Sorn

Baek

et al. 2009

Annals of the New York Academy of Sciences

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“…However saponins are its main constituents . Genotoxic activity has been not associated so far to saponins meanwhile some reports about saponis' chemopreventive and antimutagenic effects have been published (Mba Gachou et al, 1999;Panwar et al, 2005). Studies on genotoxicity of flavonoids reported both protector and inductor genotoxic effects depending on the specific compound and/or assay.…”

Section: Discussionmentioning

confidence: 99%

Toxicity and genotoxicity evaluation of Passiflora alata Curtis (Passifloraceae)

Boeira¹,

Fenner²,

Betti³

et al. 2010

Journal of Ethnopharmacology

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“…5-FU cytotoxicity could be potentiated up to 3.3-fold by 5 μM α-hederin. A previous study demonstrated that α-hederin exerted a diphasic action in human hepatocellular carcinoma HepG2 cells (Mba Gachou et al, 1999). At subtoxic concentrations, this saponin displayed a protective effect, whereas at higher concentration, it induced a membrane depolarization.…”

Section: Combination Assays Of α α α α α-Hederin With 5-fumentioning

confidence: 90%

α‐hederin potentiates 5‐FU antitumor activity in human colon adenocarcinoma cells

Bun¹,

Élias²,

Baghdikian³

et al. 2008

Phytotherapy Research

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The aim of this study was to investigate the ability of alpha-hederin to improve the efficacy of widely prescribed 5-fluorouracil (5-FU) in a human colon adenocarcinoma model. Drug combinations of alpha-hederin and 5-FU using both fixed-concentration and combination index methods were performed in vitro in HT-29 cells. The results showed that alpha-hederin at sub-IC(50) cytotoxic concentrations enhanced 5-FU cytotoxicity about 3.3-fold (p < 0.001). Simultaneous combination of alpha-hederin and 5-FU at their IC(50) ratio showed either a synergistic effect at a moderate cytotoxic range (25% of cell growth inhibition) or an antagonistic effect at a high level of growth inhibition. The data indicate therefore that it is possible to optimize colorectal cancer cell sensitivity to 5-FU with alpha-hederin.

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The protective activity of α-hederine against H2O2 genotoxicity in HepG2 cells by alkaline comet assay

Cited by 17 publications

References 28 publications

Antioxidant and Apoptotic Effects of Korean White Ginseng Extracted with the Same Ratio of Protopanaxadiol and Protopanaxatriol Saponins in Human Hepatoma HepG2 Cells

Antioxidant and Apoptotic Effects of Korean White Ginseng Extracted with the Same Ratio of Protopanaxadiol and Protopanaxatriol Saponins in Human Hepatoma HepG2 Cells

Toxicity and genotoxicity evaluation of Passiflora alata Curtis (Passifloraceae)

α‐hederin potentiates 5‐FU antitumor activity in human colon adenocarcinoma cells

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