The Protective Effect of Aromatase on NSC-34 Cells with Stably Expressed hSOD1-G93A

Liu, Yan; Qi, Wang; Liu, Yaling; Zhou, Fuling; Bai, Lin; Zhou, Xiaomeng; Sun, Can Lan; Nie, Xiangyu; Duan, Shanshan; Ran, Jina; Chen, Juan; Ji, Yingxiao; Liu, Yakun; Li, Zhongyao; Li, Yuanyuan; Wang, Qingxin

doi:10.1016/j.neuroscience.2019.05.022

Cited by 4 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is of note, however, that antibodies against aromatase have varying efficiencies. We have used an antibody previously shown to give comparable staining to two independent antibodies (Zhang et al, 2018), as well as being validated by shRNA-mediated knockdown in a previous (Yan et al, 2019) and in the current study. Nevertheless, future studies using additional antibodies as well as independent approach are needed to validate these findings.…”

Section: Discussionmentioning

confidence: 89%

“…This antibody detects a conserved region surrounding amino acid 358 of human aromatase. The antibody has recently been used for immunohistochemical studies and demonstrated comparable staining to two different and well-published aromatase antibodies in rats (Zhang et al, 2018) and has further been tested for specificity using a knockdown approach in mouse NSC-34 motor neuron-like cells (Yan et al, 2019). To confirm specificity of this antibody, hEK293 cells were transfected with a myc-DDK-tagged murine aromatase construct and exogenous aromatase expression detected using antibodies against either myc or aromatase (Figure 1b).…”

Section: Sex Differences In Aromatase Expression But Not In Migratimentioning

confidence: 97%

“…Antibodies used are as follows: GFP chicken polyclonal (ab13970; Abcam; 1:10,000); turboGFP (tGFP) rabbit polyclonal (PA5-22688, Thermo Fisher; 1:2,000); Myc mouse monoclonal (clone 2D11A8; 66004-1-lg; ProteinTech; 1:2,000); β-actin mouse monoclonal (clone 7D2C10; 60008-1-lg; ProteinTech); aromatase rabbit polyclonal (ab69653; Abcam; 1:100)-epitope is not described by the supplier; and aromatase rabbit polyclonal (ab18995; Abcam; 1:100). This antibody is raised against a peptide surrounding amino acid 385 of human aromatase, an area that is conserved across multiple species and has been reported to show similar staining to several other aromatase antibodies in rodent brain (Zhang et al, 2018) and specificity tested using ectopic expression of aromatase and an shRNA against the Cyp19a1 gene (Yan et al, 2019). A full list of antibodies used in this study can be found in Table 1.…”

Section: Reagentsmentioning

confidence: 99%

See 2 more Smart Citations

Brain‐synthesized oestrogens regulate cortical migration in a sexually divergent manner

Sellers

Saito²,

Foster

et al. 2020

Eur J of Neuroscience

View full text Add to dashboard Cite

Oestrogens play an important role in brain development where they have been implicated in controlling various cellular processes. Several lines of evidence have been presented showing that oestrogens can be synthesized locally within the brain. Studies have demonstrated that aromatase, the enzyme responsible for the conversion of androgens to oestrogens, is expressed during early development in both male and female cortices. Furthermore, 17β-oestradiol has been measured in foetal brain tissue from multiple species. 17β-oestradiol regulates neural progenitor proliferation as well as the development of early neuronal morphology. However, what role locally derived oestrogens play in regulating cortical migration and, moreover, whether these effects are the same in males and females are unknown. Here, we investigated the impact of knockdown expression of Cyp19a1, which encodes aromatase, between embryonic day (E) 14.5 and postnatal day 0 (P0) had on neural migration within the cortex. Aromatase was expressed in the developing cortex of both sexes, but at significantly higher levels in male than female mice. Under basal conditions, no obvious differences in cortical migration between male and female mice were observed. However, knockdown of Cyp19a1 resulted in an increase in cells within the cortical plate, and a concurrent decrease in the subventricular zone/ventricular zone in P0 male mice. Interestingly, the opposite effect was observed in females, who displayed a significant reduction in cells migrating to the cortical plate. Together, these findings indicate that brain-derived oestrogens regulate radial migration through distinct mechanisms in males and females. K E Y W O R D S aromatase, corticogenesis, Cyp19a1, neural development, neural stem cell, radial migration This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Sex Differences In Aromatase Expression But Not In Migratimentioning

confidence: 97%