The protective effect of chlorogenic acid on bovine mammary epithelial cells and neutrophil function

Gong, Xiaoxiao; Su, Xiaoyou; Zhan, Kai; Zhao, Guisen

doi:10.3168/jds.2017-14328

Cited by 39 publications

(27 citation statements)

References 40 publications

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“…This increase in TNF-α production is most likely explained by the observed increase in cell viability ( Figure 2 B). Similar protective effects of CGA on cell viability have been reported previously [ 37 , 38 , 39 , 40 ]. It is possible that CGA can modulate apoptotic genes in LPS stimulated THP-1 macrophages as CGA has been shown to increase the production of B-cell lymphoma-2 (Bcl-2) and decrease the production of Bax, anti-apoptotic and pro-apoptotic proteins, respectively, and decrease caspase-3 in β-amyloid stimulated PC12 cells within 1 h of treatment [ 41 ].…”

Section: Discussionsupporting

confidence: 89%

Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells

et al. 2020

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The anti-inflammatory effects of curcumin are well documented. However, the bioavailability of curcumin is a major barrier to its biological efficacy. Low-dose combination of complimentary bioactives appears to be an attractive strategy for limiting barriers to efficacy of bioactive compounds. In this study, the anti-inflammatory potential of curcumin in combination with chlorogenic acid (CGA), was investigated using human THP-1 macrophages stimulated with lipopolysaccharide (LPS). Curcumin alone suppressed TNF-α production in a dose-dependent manner with a decrease in cell viability at higher doses. Although treatment with CGA alone had no effect on TNF-α production, it however enhanced cell viability and co-administration with curcumin at a 1:1 ratio caused a synergistic reduction in TNF-α production with no impact on cell viability. Furthermore, an qRT-PCR analysis of NF-κB pathway components and inflammatory biomarkers indicated that CGA alone was not effective in reducing the mRNA expression of any of the tested inflammatory marker genes, except TLR-4. However, co-administration of CGA with curcumin, potentiated the anti-inflammatory effects of curcumin. Curcumin and CGA together reduced the mRNA expression of pro-inflammatory cytokines [TNF-α (~88%) and IL-6 (~99%)], and COX-2 (~92%), possibly by suppression of NF-κB (~78%), IκB-β-kinase (~60%) and TLR-4 receptor (~72%) at the mRNA level. Overall, co-administration with CGA improved the inflammation-lowering effects of curcumin in THP-1 cells.

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Section: Discussionsupporting

confidence: 89%

Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells

et al. 2020

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“…Therefore, PMNL play a decisive role for resolution of mastitis. The recruitment process of PMNL is controlled by chemoattractants, such as IL-8, C-C motif ligand, and C-X-C motif ligand chemokines released by resident immune cells and mammary epithelial cells [20]. In the current study, chemotactic ability of PMNL by IL-8 induction was not altered after pre-treatment of PMNL with 0.05% of Table 2 Expression of genes in PMNL incubated with 0 (control) or 0.05% tee trea oil (TTO) in vitro TTO.…”

Section: Discussionmentioning

confidence: 99%

“…Biofilm formation was evaluated as described previously [20]. Briefly, S. aureus were seeded into 96-well plates (2 × 10 6 CFU/well) and incubated at 37°C, 5% CO 2 for 24 h. Supernatants were then removed, BHI medium supplemented with 0 (control group), 0.00625%, 0.0125%, 0.025%, 0.05%, or 0.1% TTO (n = 4) added, and plates wrapped with Parafilm and incubated at 37°C, 5% CO 2 for 36 h. Next, the plates were rinsed five times with sterile distilled water, and S. aureus fixed with 100 μL of methyl alcohol for 15 min, and the supernatant removed.…”

Section: S Aureus Biofilm Formation Assaymentioning

confidence: 99%

“…To determine the number of S. aureus association to BMECs and S. aureus invasion of BMECs at different concentrations of TTO, we followed a previously described method [20]. BMECs grown in 24-well plates were infected using DMEM/F12 medium containing S. aureus (1 × 10 5 CFU/well) at MOI of 1, and treated with 0 (control group), 0.025%, 0.05%, or 0.1% TTO (n = 3) at 37°C, 5% CO 2 for 3 h. Cells were then washed five times with sterile PBS, and treated with 0.1% Triton X-100, and incubated for 15 min at 37°C, 5% CO 2 .…”

Section: S Aureus Association and Invasion Assaymentioning

confidence: 99%

“…The qRT-PCR reaction mixture contained 1 × SYBR® Premix Ex Taq™ II, 0.4 μmol/L each forward and reverse primers, and 100 ng cDNA templates in a final volume of 20 μL, and reactions were performed as follows: initial denaturation at 95°C for 30 s, followed by 40 cycles at 95°C for 5 s and 60°C for 30 s. Before the qRT-PCR for samples, the amplification efficiencies of all primers were determined by using standard dilution series. The primers listed in Table 1 are from the previous studies [19,20]. Efficiency of these primers were determined in the two study.…”

Section: Quantitative Rt-pcrmentioning

confidence: 99%

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The protective roles of tea tree oil extracts in bovine mammary epithelial cells and polymorphonuclear leukocytes

Zhan

Yang

et al. 2020

J Animal Sci Biotechnol

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Background: Tea tree oil (TTO) plays an important role in antibacterial activity and alleviating the inflammatory responses. Bovine mammary epithelium and polymorphonuclear leukocytes (PMNL) can actively respond to bovine mastitis infection. However, regulatory effects of TTO extracts on the innate immune response of bovine mammary epithelial cells (BMECs) and PMNL remain not reported. Therefore, aim of the study was to evaluate the effects of TTO extracts on the mRNA levels of the genes involved in the innate immune response of BMECs and PMNL. Results: Our results demonstrated that addition of 0.025% and 0.05% TTO increased the proliferation of BMECs, and significantly enhanced (P < 0.05) the viability of BMECs exposed to Staphylococcus aureus (S. aureus). An inhibitory effect was observed against the growth of S. aureus by TTO incubation. The 0.05% TTO reduced S. aureus biofilm formation, association and invasion of S. aureus to BMECs, and changed the morphological and structural features of S. aureus. The proinflammatory cytokines IL-1β, IL-6, and TNF-α were decreased (P < 0.001) by the incubation of TTO. Interestingly, the expression of IL-8 known for PMNL chemotactic function was elevated (P < 0.05) by 0.05% TTO treatment. Consistently, 0.05% TTO increased the migration of PMNL in S. aureus-exposed BMECs when compared with S. aureus treatment alone (P < 0.05). In addition, PMNL incubated with 0.05% TTO decreased the levels of NFKB inhibitor alpha (NFKBIA) and TNF-α. Conclusions: Our results indicate that use of TTO can relieve the BMECs pro-inflammatory response caused by S. aureus and promote the migration of PMNL to mount the innate immune responses, and it may be novel strategy for the treatment of bovine mastitis caused by S. aureus.

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Chlorogenic acid inhibits proliferation in human hepatoma cells by suppressing noncanonical NF-κB signaling pathway and triggering mitochondrial apoptosis

et al. 2021

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Chlorogenic acid (CGA), a phenylpropanoid derived from Eucommia ulmoides Oliver, has been shown to exhibit potent cytotoxic and anti-proliferative activities against several human cancers. However, the effects of CGA on hepatocellular carcinoma (HCC) and the underlying mechanisms have not been intensively studied. In this study, the CGA treatment effects on the viability of human hepatoma cells were investigated by MTT assay. Our data showed that CGA could dose-dependently inhibit the activity of human hepatoma cells Hep-G2 and Huh-7, but did not affect the activity and growth of normal human hepatocyte QSG-7701. The genes and pathways influenced by CGA treatment were explored by RNA sequencing and bioinformatics analysis, which identified 323 differentially expressed genes (DEGs) involved in multiple pharmacological signaling pathways such as MAPK, NF-κB, apoptosis and TGF-β signaling pathways. Further analyses by real-time quantitative PCR, Western blot and flow cytometry revealed that CGA effectually suppressed the noncanonical NF-κB signaling pathway, meanwhile it activated the mitochondrial apoptosis of HCC by upregulation of the BH3-only protein Bcl-2 binding component 3 (BBC3). Our findings demonstrated the potential of CGA in suppressing human hepatoma cells and provided a new insight into the anti-cancer mechanism of CGA.

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The protective effect of chlorogenic acid on bovine mammary epithelial cells and neutrophil function

Cited by 39 publications

References 40 publications

Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells

Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells

The protective roles of tea tree oil extracts in bovine mammary epithelial cells and polymorphonuclear leukocytes

Chlorogenic acid inhibits proliferation in human hepatoma cells by suppressing noncanonical NF-κB signaling pathway and triggering mitochondrial apoptosis

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