The protective effect of Eleutheroside E against the mechanical barrier dysfunction triggered by lipopolysaccharide in IPEC-J2 cells

Li, Huijuan; Han, Rui; Feng, Yong; Fan, Yueli; Zhao, Bao; Hu, Xiaocai; Zhang, Tianrui; Che, Dongsheng

doi:10.1016/j.rvsc.2022.11.002

Research in Veterinary Science

2023

DOI: 10.1016/j.rvsc.2022.11.002

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The protective effect of Eleutheroside E against the mechanical barrier dysfunction triggered by lipopolysaccharide in IPEC-J2 cells

Huijuan Li

Rui Han

Yong Feng

et al.

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Cited by 2 publications

References 30 publications

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The Effect of Clostridium butyricum‐Derived Lipoteichoic Acid on Lipopolysaccharide‐Stimulated Porcine Intestinal Epithelial Cells

Chen,

Ma,

Wen

et al. 2025

Veterinary Medicine & Sci

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BackgroundClostridium butyricum is a probiotic widely used in animal husbandry, and there is evidence to suggest that it can alleviate intestinal inflammation in pigs and may be related to its lipoteichoic acid (LTA), but the mechanism is still unclear.ObjectiveThis study aimed to determine the regulatory effect and potential mechanism of C. butyricum LTA on LPS‐stimulated inflammation in intestinal porcine epithelial line‐J2 (IPEC‐J2).MethodsIPEC‐J2 cells were treated with LPS and different concentrations of LTA (0.05, 0.1 and 0.15 mM). After treatment of 0.5, 1.5 and 4.5 h, the cell culture media were collected for the measurement of TNF‐α and IL‐10 by using ELISA kits, and the cells were collected for RT‐qPCR and Western blotting detections. Further elucidating the pathway of LTA regulating IL‐10 and TNF‐α gene expression by inhibiting key proteins in the toll‐like receptor pathway with antagonists C34, PDTC, SB230580 and U0126.ResultsHigh‐dose LTA significantly promoted the secretion of the anti‐inflammatory factor IL‐10 in IPEC‐J2 cells, and inhibited the expression and secretion of pro‐inflammatory TNF‐α in the short term. LTA inhibited the gene expression of TLR4 in LPS‐stimulated cells and reduced the protein phosphorylation levels of p38, ERK1/2 and p65. The inhibition of TLR4, p38, ERK1/2 and p65 reduced the TNF‐α gene expression caused by LPS; LTA increased TLR2 gene expression, inhibition of p38, ERK and p65 rather than TLR4 reduced the IL‐10 gene expression.ConclusionOur study found that C. butyricum LTA was an important component of C. butyricum regulating the inflammatory response of IPEC‐J2 cells. LTA mainly reduced the expression of TNF‐α by inhibiting TLR4, while stimulating TLR2 increased the expression of IL‐10. Downstream p65, p38 and ERK1/2 were involved in regulating both TNF‐α and IL‐10. However, TLR4 was only related to the increase in TNF‐α caused by LPS and not to the increase in IL‐10 caused by LTA. Our work supplemented the probiotic mechanism of C. butyricum and provided a theoretical basis for the application of C. butyricum LTA.

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The Effect of Clostridium butyricum‐Derived Lipoteichoic Acid on Lipopolysaccharide‐Stimulated Porcine Intestinal Epithelial Cells

Chen,

Ma,

Wen

et al. 2025

Veterinary Medicine & Sci

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Zang Siwei Qingfei Mixture Alleviates Inflammatory Response to Attenuate Acute Lung Injury by the ACE2/NF-κB Signaling Pathway in Mice

Lei,

2024

CCHTS

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Background:: Acute lung injury (ALI) is a serious lung disease characterized by acute and severe inflammation. Upregulation of ACE2 and inhibition of the NF-κB signaling pathway attenuate LPS-induced ALI. Objective:: To explore whether Zang Siwei Qingfei Mixture inhibits the development of ALI through the ACE2/NF-κB signaling pathway. Methods:: Alveolar type II epithelial cells (AEC II) were identified by immunofluorescence staining and flow cytometry. C57BL/6J mice were treated with LPS to establish an ALI model. Cell viability was assessed using CCK8 assays. The levels of ACE, ACE2, p-p38/p38, p- ERK1/2/ERK1/2, p-JNK/JNK, p-IκBα/IκB-α, p-NF-κBp65 were analyzed by Western blotting. ELISA was applied to detect the levels of TNF-a, IL-6, AGT, and Ang1-7. HE staining was used to observe lung injury. The mRNA expression of ACE, ACE2, and Mas was measured by RTqPCR. Results:: AEC II cells were successfully isolated. Treatment with Zang Siwei Qingfei mixture resulted in a decrease in ACE, p-p38/p38, p-ERK1/2/ERK1/2, p-JNK/JNK, p-IκBα/IκB-α, p- NF-κBp65 levels, while increasing ACE2 levels. Zang Siwei Qingfei mixture also led to a reduction in TNF-α, IL6, and AGT levels, while increasing Ang1-7 level. Histological analysis showed that Zang Siwei Qingfei Mixture treatment improved the alveolar structure of ALI mice and reduced inflammatory infiltration. The pretreatment with MLN-4760, an ACE2 inhibitor, resulted in opposite effects compared to Zang Siwei Qingfei Mixture treatment. Conclusion:: Zang Siwei Qingfei mixture attenuates ALI by regulating the ACE2/NF-κB signaling pathway in mice. This study provides a theoretical foundation for the development of improved ALI treatments.

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The protective effect of Eleutheroside E against the mechanical barrier dysfunction triggered by lipopolysaccharide in IPEC-J2 cells

Cited by 2 publications

References 30 publications

The Effect of Clostridium butyricum‐Derived Lipoteichoic Acid on Lipopolysaccharide‐Stimulated Porcine Intestinal Epithelial Cells

The Effect of Clostridium butyricum‐Derived Lipoteichoic Acid on Lipopolysaccharide‐Stimulated Porcine Intestinal Epithelial Cells

Zang Siwei Qingfei Mixture Alleviates Inflammatory Response to Attenuate Acute Lung Injury by the ACE2/NF-κB Signaling Pathway in Mice

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