The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification

Sun, Wu-Sheng; Jang, Hoon; Kwon, Hyo J.; Kim, Ki Young; Ahn, Soo Bin; Hwang, Seongsoo; Lee, Sung Gu; Lee, Jun Hyuck; Hwang, In-Sul; Lee, Jeong-Woong

doi:10.1016/j.theriogenology.2020.04.016

Cited by 13 publications

(7 citation statements)

References 55 publications

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“…Similar favourable results were obtained in the vitrification of immature murine oocytes with AFP III [ 65 , 66 ] and bovine oocytes with AFGP-8 [ 67 ] and Glaciozyma sp. ice-binding protein (LeIBP) [ 68 ].…”

Section: Applications Of Afps In Cryopreservationmentioning

confidence: 99%

“…Li et al included Anatolica polita -derived AFP to the controlled slow freezing versus vitrification Sheep embryos where vitrification resulted in higher survival and embryo hatching rates, and 10 μg/mL of the AFP promoted hatching of slow-growing vitrified embryos [ 70 ]. Similarly successful applications include AFP III in rabbit [ 71 ] and turbot ( Scophthalmus maximus ) [ 72 ] embryos, AFP I and III in zebrafish embryo [ 73 , 74 ], AFP I in seabream ( Sparus aurata ) [ 75 ] and sheep embryos [ 76 ], LeIBP in bovine embryos [ 68 ]. To the best of our knowledge, no research evidence that supports the use of AFP in human cryobanking of human oocytes or embryos has been reported at the time of this review.…”

Section: Applications Of Afps In Cryopreservationmentioning

confidence: 99%

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Antifreeze Proteins: Novel Applications and Navigation towards Their Clinical Application in Cryobanking

Ekpo

Xie

et al. 2022

IJMS

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Antifreeze proteins (AFPs) or thermal hysteresis (TH) proteins are biomolecular gifts of nature to sustain life in extremely cold environments. This family of peptides, glycopeptides and proteins produced by diverse organisms including bacteria, yeast, insects and fish act by non-colligatively depressing the freezing temperature of the water below its melting point in a process termed thermal hysteresis which is then responsible for ice crystal equilibrium and inhibition of ice recrystallisation; the major cause of cell dehydration, membrane rupture and subsequent cryodamage. Scientists on the other hand have been exploring various substances as cryoprotectants. Some of the cryoprotectants in use include trehalose, dimethyl sulfoxide (DMSO), ethylene glycol (EG), sucrose, propylene glycol (PG) and glycerol but their extensive application is limited mostly by toxicity, thus fueling the quest for better cryoprotectants. Hence, extracting or synthesizing antifreeze protein and testing their cryoprotective activity has become a popular topic among researchers. Research concerning AFPs encompasses lots of effort ranging from understanding their sources and mechanism of action, extraction and purification/synthesis to structural elucidation with the aim of achieving better outcomes in cryopreservation. This review explores the potential clinical application of AFPs in the cryopreservation of different cells, tissues and organs. Here, we discuss novel approaches, identify research gaps and propose future research directions in the application of AFPs based on recent studies with the aim of achieving successful clinical and commercial use of AFPs in the future.

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Section: Applications Of Afps In Cryopreservationmentioning

confidence: 99%

Section: Applications Of Afps In Cryopreservationmentioning

confidence: 99%

Antifreeze Proteins: Novel Applications and Navigation towards Their Clinical Application in Cryobanking

Ekpo

Xie

et al. 2022

IJMS

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“…IVF was performed as described previously [29]. Briefly, a 0.5-mL straw of Hanwoo semen was thawed at 37 • C for 30 s; then, the contents in the straw were layered onto the top of a Percoll density gradient consisting of 2 mL of 45% Percoll above 2 mL of 90% Percoll in a 15 mL conical tube.…”

Section: In Vitro Fertilization (Ivf) and In Vitro Culture (Ivc)mentioning

confidence: 99%

“…The intracellular ROS content was measured according to our previous study [29]. In brief, denuded oocytes were incubated in DPBS containing 0.4% (w/v) polyvinyl alcohol (DPBS-PVA) and 10 µM 2 ,7 -dichlorofluorescein diacetate (H 2 DCFDA) at 38.5 • C in 5% CO 2 for 30 min in the dark.…”

Section: Measurement Of Intracellular Ros Gsh Levels and Mitochondria...mentioning

confidence: 99%

N-acetyl-L-cysteine Improves the Developmental Competence of Bovine Oocytes and Embryos Cultured In Vitro by Attenuating Oxidative Damage and Apoptosis

et al. 2021

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Oxidative stress has been suggested to negatively affect oocyte and embryo quality and developmental competence, resulting in failure to reach full term. In this study, we investigated the effect of N-acetyl-L-cysteine (NAC), a cell-permeating antioxidant, on developmental competence and the quality of oocytes and embryos upon supplementation (0.1–10 mM) in maturation and culture medium in vitro using slaughterhouse-derived oocytes and embryos. The results show that treating oocytes with 1.0 mM NAC for 8 h during in vitro maturation attenuated the intracellular reactive oxygen species (ROS) (p < 0.05) and upregulated intracellular glutathione levels (p < 0.01) in oocytes. Interestingly, we found that NAC affects early embryonic development, not only in a dose-dependent, but also in a stage-specific, manner. Significantly (p < 0.05) decreased cleavage rates (90.25% vs. 81.46%) were observed during the early stage (days 0–2), while significantly (p < 0.05) increased developmental rates (38.20% vs. 44.46%) were observed during the later stage (from day 3) of embryonic development. In particular, NAC supplementation decreased the proportion of apoptotic blastomeres significantly (p < 0.05), resulting in enhanced hatching capability and developmental rates during the in vitro culture of embryos. Taken together, our results suggest that NAC supplementation has beneficial effects on bovine oocytes and embryos through the prevention of apoptosis and the elimination of oxygen free radicals during maturation and culture in vitro.

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“…In particular, embryos are very difficult to cryopreserve because of their size, survival sensitivity, and multi-compositional structure [ 7 , 8 ]. Several studies have reported that aberrant effects such as genotoxicity, necrosis, and abnormal apoptotic activity are responsible for the cytotoxicity observed in gametes and embryos during cryopreservation and vitrification [ 9 , 10 , 11 , 12 ]. To overcome these impediments, a number of researchers have studied the effect of co-treatment of gametes and embryos with protective adjuvants and freezing media, which was found to reduce toxicity as well as increase the success rate of artificial insemination.…”

Section: Introductionmentioning

confidence: 99%

Application of Nanoparticles and Melatonin for Cryopreservation of Gametes and Embryos

Choi

Jang

2022

CIMB

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Cryopreservation of gametes and embryos, a technique widely applied in human infertility clinics and to preserve desirable genetic traits of livestock, has been developed over 30 years as a component of the artificial insemination process. A number of researchers have conducted studies to reduce cell toxicity during cryopreservation using adjuvants leading to higher gamete and embryo survival rates. Melatonin and Nanoparticles are novel cryoprotectants and recent studies have investigated their properties such as regulating oxidative stresses, lipid peroxidation, and DNA fragmentation in order to protect gametes and embryos during vitrification. This review presented the current status of cryoprotectants and highlights the novel biomaterials such as melatonin and nanoparticles that may improve the survivability of gametes and embryos during this process.

show abstract

The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification

Cited by 13 publications

References 55 publications

Antifreeze Proteins: Novel Applications and Navigation towards Their Clinical Application in Cryobanking

Antifreeze Proteins: Novel Applications and Navigation towards Their Clinical Application in Cryobanking

N-acetyl-L-cysteine Improves the Developmental Competence of Bovine Oocytes and Embryos Cultured In Vitro by Attenuating Oxidative Damage and Apoptosis

Application of Nanoparticles and Melatonin for Cryopreservation of Gametes and Embryos

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