The protective effect of nigeglanine on dextran sulfate sodium‐induced experimental colitis in mice and Caco‐2 cells

Abstract

Ulcerative colitis (UC) was a nonspecific inflammatory disease. The treatment of UC is imperative. The present study aimed to investigate the effect of nigeglanine on dextran sulfate sodium-induced UC in experimental mice and Caco-2 cells and define the underlying mechanism. The nigeglanine was shown a significant protective effect on the colon, significantly reduced the weight and colon length loss and inhibited intestinal epithelial cell damage. Nigeglanine also reduced proinflammatory factors and increased anti-inflammatory factor production. The results indicate that nigeglanine suppresses the nuclear factor kappa B and mitogen-activated protein kinases pathways in addition to NLRP3 inflammasome action, inhibiting colon epithelial cell pyroptosis. Surprisingly, ZO-1 and occludin protein levels increased with nigeglanine treatment, suggesting that nigeglanine plays a protective role in barrier integrity, reducing colitis progression. The present study suggests that dietary therapy with nigeglanine may be a useful treatment for prophylaxis and palliative UC. K E Y W O R D Sdietary therapy, inflammatory factor, nigeglanine, NLRP3 inflammasome, ulcerative colitis

Shen-Ling-Bai-Zhu-San Improves Dextran Sodium Sulfate-Induced Colitis by Inhibiting Caspase-1/Caspase-11-Mediated Pyroptosis

“…Our experimental results demonstrate that SLBZS has a therapeutic effect on DSS-induced colitis in mice. The experimental colitis model is characterized by weight loss, bloody diarrhea, inflammatory mediator production, and intestinal mucosal immune cell infiltration (Gao et al, 2019;Zhang et al, 2019). However, there is currently no high-quality study of SLBZS in DSS-induced colitis.…”

Polyphenol Rich Forsythia suspensa Extract Alleviates DSS-Induced Ulcerative Colitis in Mice through the Nrf2-NLRP3 Pathway

“…Pyroptosis is a type of programmed cell death that is involved in experimental colitis in mice [23,24]. Therefore, to explore whether Forsythia suspensa extract exerts anti-inflammatory effects by inhibiting cell pyroptosis, we measured the expression of pyroptosis-related genes.…”

Anti-inflammatory and protective effects of Pimpinella candolleana on ulcerative colitis in rats: a comprehensive study of quality, chemical composition, and molecular mechanisms

“…Diet and water were given after 4 h. At 9:00 a.m. every day, rats in the model group were intragastrically administered physiological saline, while rats in the positive medication group were intragastrically administered 5 mg/kg sulfasalazine, and low, middle and high doses of P. candolleana were given 600 mg/kg, 1200 mg/kg and 1800 mg/kg, respectively, for 14 days. Acetic acidinduced colitis is a commonly used animal model that closely mimics human UC (Gao et al, 2019). After P. candolleana intervention, there were no deaths in the normal group (NC), while the mortality rate in the positive control group (SG) was 62.5%.…”