The protective effect of Stevia rebaudiana Bertoni on serum hormone levels, key steroidogenesis enzymes, and testicular damage in testes of diabetic rats

Gholizadeh, Fatemeh; Dastghaib, Sanaz; Koohpeyma, Farhad; Bayat, Elahe; Mokarram, Pooneh

doi:10.1016/j.acthis.2019.08.001

Cited by 19 publications

(10 citation statements)

References 45 publications

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“…Consistent with current results, Gholizadeh et al [ 136 ] reported that MTF improved many aspects of diabetes-induced testicular dysfunction such as the testis weight and volume and sperm count and motility. In line with that, Faure et al [ 137 ] reported that MTF has critical effects on steroidogenesis and spermatogenesis in testicular tissue, particularly through the activation of the AMPK pathway, which induces lactate production without affecting lactate dehydrogenase concentration in cultured Sertoli cells, though the exact molecular mechanism and the action of MTF remains unclear.…”

Section: Resultssupporting

confidence: 91%

Sustained In Vitro and In Vivo Delivery of Metformin from Plant Pollen-Derived Composite Microcapsules

2021

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We developed a dual microencapsulation platform for the type 2 diabetes drug metformin (MTF), which is aimed to increase its bioavailability. We report the use of Lycopodium clavatum sporopollenin (LCS), derived from their natural spores, and raw Phoenix dactylifera L. (date palm) pollens (DPP) for MTF microencapsulation. MTF was loaded into LCS and DPP via a vacuum and a novel method of hydration-induced swelling. The loading capacity (LC) and encapsulation efficiency (EE) percentages for MTF-loaded LCS and MTF-loaded DPP microcapsules were 14.9% ± 0.7, 29.8 ± 0.8, and 15.2% ± 0.7, 30.3 ± 1.0, respectively. The release of MTF from MTF-loaded LCS microcapsules was additionally controlled by re-encapsulating the loaded microcapsules into calcium alginate (ALG) microbeads via ionotropic gelation, where the release of MTF was found to be significantly slower and pH-dependent. The pharmacokinetic parameters, obtained from the in vivo study, revealed that the relative bioavailability of the MTF-loaded LCS-ALG beads was 1.215 times higher compared to pure MTF, following oral administration of a single dose equivalent to 25 mg/kg body weight MTF to streptozotocin (STZ)-induced diabetic male Sprague-Dawley rats. Significant hypoglycemic effect was obtained for STZ-induced diabetic rats orally treated with MTF-loaded LCS-ALG beads compared to control diabetic rats. Over a period of 29 days, the STZ-induced diabetic rats treated with MTF-loaded LCS-ALG beads showed a decrease in the aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides, cholesterol, and low-density lipoprotein-cholesterol (LDL-C) levels, as well as an increase in glutathione peroxidase (GPx) and a recovery in the oxidative stress biomarker, lipid peroxidation (LPx). In addition, histopathological studies of liver, pancreas, kidney, and testes suggested that MTF-loaded LCS-ALG beads improved the degenerative changes in organs of diabetic rats. The LCS-ALG platform for dual encapsulation of MTF achieved sustained MTF delivery and enhancement of bioavailability, as well as the improved biochemical and histopathological characteristics in in vivo studies, opening many other intriguing applications in sustained drug delivery.

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Section: Resultssupporting

confidence: 91%

Sustained In Vitro and In Vivo Delivery of Metformin from Plant Pollen-Derived Composite Microcapsules

2021

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“…Cholesterol is converted into sex hormones after a series of enzymatic reactions (Zhang, Rodriguez, & Mellon, 1995). StAR protein can accelerate the cholesterol transport process (Gholizadeh, Dastghaib, Koohpeyma, Bayat, & Mokarram, 2019), thereby accelerating this rate‐limiting step (Amory & Bremner, 2003). Steroid production first converts cholesterol to pregnenolone (Stocco, 2001), after which cytochrome P450scc catalyzes this process (Lin et al., 1998), which also represents the rate‐limiting step during steroid hormone biosynthesis (Omura & Morohashi, 1995).…”

Section: Discussionmentioning

confidence: 99%

Section: Amino Acidmentioning

confidence: 99%

The beneficial effect of oyster peptides and oyster powder on cyclophosphamide‐induced reproductive impairment in male rats: A comparative study

Zhou

Wei

et al. 2020

J. Food Biochem.

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In addition to being nutritional elements, proteins in food can also regulate the body's physiological functions, which is achieved via specific peptide sequences in the proteins (Chakrabarti, Guha, & Majumder, 2018). Therefore, although many bioactive peptides are stored in dietary proteins, the peptides remain inactive in the protein sequence and only have specific physiological functions after being released (Kitts & Weiler, 2003). Proteases from various sources can release biologically active peptides (Korhonen & Pihlanto, 2006), which are absorbed by the body via a different absorption route than the protein (Silk, Grimble, & Rees, 1985). Bioactive peptides can be absorbed directly from the intestine into the bloodstream, where they are highly active, exhibiting physiological effects in small amounts. The bioactive peptide activity is controlled by the composition and sequence of amino acids (Chakrabarti et al., 2018), and many peptides are known to reveal multifunctional properties

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“…Metformin is an oral biguanide insulin-sensitizing drug used over the past 50 years to treat type 2 DM (103). In a few studies, treatment with metformin in diabetic male rats enhanced male reproductive parameters, such as serum and intratesticular testosterone levels, Leydig cell count, improved sperm quality parameters, and decreased sperm nuclear DNA fragmentation (104)(105)(106)(107)(108)(109). Furthermore, a study performed on oligo-terato-asthenozoospermic patients with MetS treated with metformin showed an increased serum androgen levels and consequently an enhancement of semen quality (110).…”

Section: Pharmacological Treatment Of Dm With Metformin and Its Implication On Sperm Quality And Art Outcomesmentioning

confidence: 99%

Assisted reproductive technology outcomes in obese and diabetic men: lighting the darkness

et al. 2021

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The prevalence of obesity and diabetes, two of the most prevalent metabolic disorders (MetDs) in the world, has been rising exponentially over the last two decades. In addition to other comorbidities, MetDs have a detrimental impact on reproductive features, leading to a boost of the use of assisted reproductive technologies (ARTs) to overcome fertility problems. Although ARTs help to improve MetD male reproductive outcomes, data show that the results are less successful compared with those of men without MetD. Currently, intracytoplasmic sperm injection is the election procedure to bypass infertility in men with MetD. Nevertheless, embryos obtained by intracytoplasmic sperm injection using spermatozoa of men with MetD have a lower probability to end in a live birth. This embryo development shutdown has been related to a higher rate of spermatozoa with fragmented DNA and with modifications on pathways that do not allow embryos to go further in the development process. This special detrimental feature of sperm from men with MetD indicates that advanced sperm selection techniques should be used in these patients to avoid sperm with fragmented DNA. Fortunately, sperm selection procedures are under constant development and eventually will allow physicians to select spermatozoa with higher quality and low DNA fragmentation to be used in further ART, increasing the outcome of those procedures. Future research should be performed to enlighten alterations in embryos derived from spermatozoa of men with MetD.

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The protective effect of Stevia rebaudiana Bertoni on serum hormone levels, key steroidogenesis enzymes, and testicular damage in testes of diabetic rats

Cited by 19 publications

References 45 publications

Sustained In Vitro and In Vivo Delivery of Metformin from Plant Pollen-Derived Composite Microcapsules

Sustained In Vitro and In Vivo Delivery of Metformin from Plant Pollen-Derived Composite Microcapsules

The beneficial effect of oyster peptides and oyster powder on cyclophosphamide‐induced reproductive impairment in male rats: A comparative study

Assisted reproductive technology outcomes in obese and diabetic men: lighting the darkness

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