The Protective Effects of 5-Methoxytryptamine-α-lipoic Acid on Ionizing Radiation-Induced Hematopoietic Injury

Abstract

Antioxidants are prospective radioprotectors because of their ability to scavenge radiation-induced reactive oxygen species (ROS). The hematopoietic system is widely studied in radiation research because of its high radiosensitivity. In the present study, we describe the beneficial effects of 5-methoxytryptamine-α-lipoic acid (MLA), which was synthesized from melatonin and α-lipoic acid, against radiation-induced hematopoietic injury. MLA administration significantly enhanced the survival rate of mice after 7.2 Gy total body irradiation. The results showed that MLA not only markedly increased the numbers and clonogenic potential of hematopoietic cells but also decreased DNA damage, as determined by flow cytometric analysis of histone H2AX phosphorylation. In addition, MLA decreased the levels of ROS in hematopoietic cells by inhibiting NOX4 expression. These data demonstrate that MLA prevents radiation-induced hematopoietic syndrome by increasing the number and function of and by inhibiting DNA damage and ROS production in hematopoietic cells. These data suggest MLA is beneficial for the protection of radiation injuries.

Effects of Thymoquinone on radiation enteritis in mice

“…Radiation produces DNA damage directly through reactive oxygen species 33 , and destroys the expression of proteins in cells 34 , activating p53 4 , 33 , 35 , 36 . In addition, DNA injury induced by IR was determined by γH2AX phosphorylation, and this was used as an indicator for quantifying DNA double-strand breaks 37 . Recent studies suggest that p53 in intestinal epithelial cells principally controls radiation-induced GI toxicity in mice, independently of apoptosis 4 .…”

Radioprotective effects of dammarane sapogenins against 60Co‐induced myelosuppression in mice

“…Radiotherapy for cancer treatment increased the risk of myelosuppression. Irradiation may cause dysfunction in multiple cells and various tissues, especially hematopoietic failure and immune impairment (Kim et al, ; Li et al, ). Bone marrow is the most important hematopoietic organ and highly susceptible to irradiation injury, because it keeps a high rate of cell proliferation and composes different developmental stages of hematopoietic cells (Bentzen, ).…”

The protective effects of XH‐105 against radiation‐induced intestinal injury

“…13 Therefore, development of an effective method and drug to mitigate the radiation-induced intestinal injuries needs to be explored. Many studies have reported that Chinese herbal medicines or extracts and other drugs may be able to reduce TBI-induced injuries in the brain, oesophagus and haematopoietic system of irradiated animals, [14][15][16][17][18] but the study of protective drugs in IR-induced intestinal injuries still needs to be improved. [19][20][21] In the present study, we observed that the new compound XH-105 had protective effects on radiation-induced intestinal injuries.…”