The protective effects of electroacupuncture on intestinal barrier lesions in IBS and UC model

Liu, Shuqing; Huang, Qin; Huang, Qizhao; Wang, Yuemei; Li, Sihui; Wang, Jun-Meng; Wu, Qiaofeng

doi:10.1038/s41598-023-34182-z

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…Some scholars have suggested that changes in goblet cell secretion in IBS can disrupt the intestinal chemical barrier. 33 , 37 We verified and obtained results showing a decrease in MUC2 expression in goblet cell secretion in IBS model. In comparison to IBS group, following TXYF treatment, the expression of MUC2 in goblet cells increased.…”

Section: Discussionsupporting

confidence: 67%

Tongxieyaofang Decotion Alleviates IBS by Modulating CHRM3 and Gut Barrier

Feng,

Zhou,

et al. 2024

DDDT

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Purpose Through network pharmacology combined with molecular docking and in vivo validation, the study examines the unexplored molecular mechanisms of Tongxieyaofang (TXYF) in the treatment of irritable bowel syndrome (IBS). In particular, the potential pharmacological mechanism of TXYF alleviating IBS by regulating CHRM3 and intestinal barrier has not been studied. Patients and Methods LC-MS technique and TCMSP database were used in combination to identify the potential effective components and target sites of TXYF. Potential targets for IBS were obtained from Genecards and OMIM databases. PPI and cytoHub analysis for targets. Molecular docking was used to validate the binding energy of effective components with related targets and for visualization. GO and KEGG analysis were employed to identify target functions and signaling pathways. In the in vivo validation, wrap restraint stress-induced IBS model was employed to verify the change for cytoHub genes and CHRM3 expression. Furthermore, inflammatory changes of colon were observed by HE staining. The changes of Ach were verified by ELISA. IHC and WB validated CHRM3 and GNAQ/PLC/MLCK channel variations. AB-PAS test and WB test confirmed the protection of TXYF on gut barrier. The NF-κB/MLCK pathway was also verified. Results In TXYF decoction, LC-MS identified 559 chemical components, with 23 remaining effective components after screening in TCMSP. KEGG analysis indicated that calcium plays a crucial role in TXYF treated for IBS. Molecular docking validated the binding capacity of the effective components Naringenin and Nobiletin with cytoHub-gene and CHRM3. In vivo validation demonstrated that TXYF inhibits the activation of Ach and CHRM3 in IBS, and inhibits for the GNAQ/PLC/MLCK axis. Additionally, TXYF downregulates TNF-α, MMP9, and NF-κB/MLCK, while modulating goblet cell secretion to protect gut barrier. Conclusion TXYF inhibits Ach and CHRM3 expression, regulating the relaxation of intestinal smooth muscle via GNAQ/PLC/MLCK. Additionally, TXYF inhibits NF-κB/MLCK activated and goblet cell secretion to protect gut barrier.

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Section: Discussionsupporting

confidence: 67%

Tongxieyaofang Decotion Alleviates IBS by Modulating CHRM3 and Gut Barrier

Feng,

Zhou,

et al. 2024

DDDT

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“…After 5 days of UC modeling, mice in the DSS + EA group were treated with EA once a day for 5 days ( 19 ). A custom-made mouse frame was used to hold the animals and both of the Zusanli acupoint points (ST36, located ~3 mm below the capitulum fibulae) were targeted by EA for 30 minutes (2 Hz, 0.2 mA) ( 20 ), the hind limbs of the mice began to tremble slightly. Similarly, the control and the DSS groups were held for 30 minutes without EA intervention.…”

Section: Methodsmentioning

confidence: 99%

Electroacupuncture alleviates ulcerative colitis by targeting CXCL1: evidence from the transcriptome and validation

Zhang,

Dong,

Shen

et al. 2023

Front. Immunol.

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BackgroundWe aimed to use transcriptomics, bioinformatics analysis, and core gene validation to identify the core gene and potential mechanisms for electroacupuncture (EA) treatment of ulcerative colitis (UC).Materials and methodsEA was performed in mice after induction of UC via dextran sodium sulfate. Body weight, disease activity index (DAI), colon length, and hematoxylin-eosin of the colon tissue were used to evaluate the effects of EA. Mice transcriptome samples were analyzed to identify the core genes, and further verified with human transcriptome database; the ImmuCellAI database was used to analyze the relationship between the core gene and immune infiltrating cells (IICs); and immunofluorescence was used to verify the results.ResultsEA could reduce DAI and histological colitis scores, increase bodyweight and colon length, and improve the expression of local and systemic proinflammatory factors in the serum and colon of UC mice. Eighteen co-differentially expressed genes were identified by joint bioinformatics analyses of mouse and human transcriptional data; Cxcl1 was the core gene. EA affected IICs by inhibiting Cxcl1 expression and regulated the polarization of macrophages by affecting the Th1 cytokine IFN-γ, inhibiting the expression of CXCL1.ConclusionsCXCL1 is the target of EA, which is associated with the underlying immune mechanism related to Th1 cytokine IFN-γ.

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A Randomized Controlled Trial Assessing the Impact of Transcutaneous Electrical Acupoint Stimulation on Gastrointestinal Motility, Nutritional Status, and Immune Function in Patients Following Cerebrovascular Accident Surgery

Chen,

Liu

et al. 2024

Journal of Investigative Surgery

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The protective effects of electroacupuncture on intestinal barrier lesions in IBS and UC model

Cited by 3 publications

References 43 publications

Tongxieyaofang Decotion Alleviates IBS by Modulating CHRM3 and Gut Barrier

Tongxieyaofang Decotion Alleviates IBS by Modulating CHRM3 and Gut Barrier

Electroacupuncture alleviates ulcerative colitis by targeting CXCL1: evidence from the transcriptome and validation

A Randomized Controlled Trial Assessing the Impact of Transcutaneous Electrical Acupoint Stimulation on Gastrointestinal Motility, Nutritional Status, and Immune Function in Patients Following Cerebrovascular Accident Surgery

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