The Protective Effects of Sika Deer Antler Protein on Cisplatin-Induced Nephrotoxicity

Abstract

Background/Aims: This study measured the effect of Sika deer (Cervus nippon Temminck) antler protein (SDAPR), glycoproteins (SDAG), and polysaccharides (SDAPO) on cisplatin-induced cytotoxicity in HEK 293 cells, and investigated the effect of SDAPR against cisplatin-induced nephrotoxicity in mice. Methods: Cell viability was measured by MTT assay. ICR mice were randomly divided into five groups: control, cisplatin with vehicle, and cisplatin with SDAPR at three concentrations: 5, 10, or 20 mg/kg, p.o., 10 d. Cisplatin was injected on 7^th day (25 mg/kg, i.p.). Renal function, oxidative stress, levels of inflammatory factors, and expression of apoptosis-related proteins were measured in vivo. Renal tissues were stained with TUNEL and H&E to observe renal cell apoptosis and pathological changes. Results: Pretreatment with SDAPR (125-2000 µg/mL) significantly improved cell viability, with an EC₅₀ of approximately 1000 µg/mL. SDAPR also ameliorated cisplatin-induced histopatholo- gic changes, and decreased blood urea nitrogen (BUN) and creatinine (Cr) (P < 0.05). Western blotting analysis showed SDAPR clearly decreased expression levels of cleaved-caspase-3 and Bax, and increased the expression level of Bcl-2 (P < 0.01). Additionally, SDAPR markedly regulated oxidative stress markers and inflammatory cytokines (P<0.05). TUNEL staining showed decreased apoptosis after SDAPR treatment (P < 0.01). Conclusions: These results indicate that SDAPR can be an effective dietary supplement, to relieve cisplatin-induced nephrotoxicity by improved antioxidase activity, suppressed inflammation, and inhibited apoptosis in vivo.

Pseudoginsengenin DQ Exhibits Therapeutic Effects in Cisplatin-Induced Acute Kidney Injury via Sirt1/NF-κB and Caspase Signaling Pathway without Compromising Its Antitumor Activity in Mice

“…It was reported that oxidative stress and inflammation are also leading factors in CDDP-induced nephrotoxicity [ 10 ]. As CDDP exposure causes renal dysfunction, accompanying oxygen-derived free radicals (ROS) lead to oxidative stress, such as excessive lipid peroxidation, which results in a response of numerous proinflammatory cytokines and inflicts irreversible cellular damage in the kidney [ 11 , 12 , 13 ]. Sirtuin-1 (Sirt-1) is an important protein that is involved in the biological processes of CDDP-induced oxidative stress and inflammation by activating transcriptional activity of downstream genes, including nuclear-related factor 2 (Nrf2) and nuclear factor-kappa B (NF-κB) [ 14 , 15 ].…”

The Protective Effect of Sika Deer Antler Protein on Gentamicin-Induced Nephrotoxicity in Vitro and in Vivo

“…In previous studies, we found an effect which GM administration could significantly increase levels of serum BUN and Cr after GM injected [24][25][26]. Conversely, the higher BUN and Cr as an indicator of kidney dysfunction in GM-induced mice recovered to normal levels after co-treatment with SDAPR.…”

Hydration with Mannitol and Dextrose May Promote Cisplatin-Induced Nephrotoxicity: Test of Five Protocols of Hydration during Cisplatin Therapy in Rat Models

“…(1) Groups 1 (male, n � 6) and 8 injection, the combination of NS (12.5 ml kg, ip) and furosemide (F, 2.5 ml/kg, ip) was administrated. (4) Groups 5 (male, n � 6) and 12 (female, n � 6) are named M, NS + CP + NS.…”

“…Several studies have been done in order to assess the protective effect of different synthetic and herbal antioxidants supplements against CP-induced nephrotoxicity [7][8][9][10][11]. In addition, other studies have been tried to alleviate the adverse effect of CP-induced nephropathy [12][13][14].…”