The protective effects of thymoquinone on lung damage caused by cigarette smoke

Yetkin, Nur Aleyna; Büyükoğlan, Hakan; Sönmez, Mehmet Fatih; Tutar, Nuri; Gülmez, İnci; Yılmaz, İnsu

doi:10.1080/10520295.2019.1681511

Cited by 13 publications

(15 citation statements)

References 30 publications

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“…The previous study explained the anti-inflammatory effect of thymoquinone and reported that IL-1β and IL-8 levels were elevated in the group exposed to cigarette smoke compared to the control group. Moreover, IL-8 levels declined in the group that received only TQ, which designated the anti-inflammatory effect of TQ [ 28 ]. Moreover, another study reported that TQ decreased the secretion of serum TNF-α and reduced the lung histopathological alternations induced by cyclophosphamide [ 29 ] and other cytokine levels [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Thymoquinone, an Active Compound of Nigella sativa, on Rats with Benzo(a)pyrene-Induced Lung Injury through Regulation of Oxidative Stress and Inflammation

et al. 2021

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Benzopyrene [B(a)P] is a well-recognized environmental carcinogen, which promotes oxidative stress, inflammation, and other metabolic complications. In the current study, the therapeutic effects of thymoquinone (TQ) against B(a)P-induced lung injury in experimental rats were examined. B(a)P used at 50 mg/kg b.w. induced lung injury that was investigated via the evaluation of lipid profile, inflammatory markers, nitric oxide (NO), and malondialdehyde (MDA) levels. B(a)P also led to a decrease in superoxide dismutase (SOD) (34.3 vs. 58.5 U/mg protein), glutathione peroxidase (GPx) (42.4 vs. 72.8 U/mg protein), catalase (CAT) (21.2 vs. 30.5 U/mg protein), and total antioxidant capacity compared to normal animals. Treatment with TQ, used at 50 mg/kg b.w., led to a significant reduction in triglycerides (TG) (196.2 vs. 233.7 mg/dL), total cholesterol (TC) (107.2 vs. 129.3 mg/dL), and inflammatory markers and increased the antioxidant enzyme level in comparison with the group that was administered B(a)P only (p < 0.05). B(a)P administration led to the thickening of lung epithelium, increased inflammatory cell infiltration, damaged lung tissue architecture, and led to accumulation of collagen fibres as studied through haematoxylin and eosin (H&E), Sirius red, and Masson’s trichrome staining. Moreover, the recognition of apoptotic nuclei and expression pattern of NF-κB were evaluated through the TUNEL assay and immunohistochemistry, respectively. The histopathological changes were found to be considerably low in the TQ-treated animal group. The TUNEL-positive cells increased significantly in the B(a)P-induced group, whereas the TQ-treated group showed a decreased apoptosis rate. Significantly high cytoplasmic expression of NF-κB in the B(a)P-induced group was seen, and this expression was prominently reduced in the TQ-treated group. Our results suggest that TQ can be used in the protection against benzopyrene-caused lung injury.

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Thymoquinone, an Active Compound of Nigella sativa, on Rats with Benzo(a)pyrene-Induced Lung Injury through Regulation of Oxidative Stress and Inflammation

et al. 2021

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“…Preventive effect of constituents of N. sativa on COPD, experimental studies Histopathological changes in the lung tissue of rats induced by cigarette smoke (a rat model of COPD) including hemorrhage, bronchiole bleeding, venous congestion, loss of epithelial cells, alveolar expansion and large cells with pale cytoplasm were ameliorated by treatment of TQ (5 mg/kg, i.p.). The levels of IL-8, IL-6, IL-1β and MMP-9 in the serum and lung tissue were also decreased due to TQ treatment (Yetkin et al, 2020).…”

Section: Copd Experimental Studiesmentioning

confidence: 94%

The effects of Nigella sativa on respiratory, allergic and immunologic disorders, evidence from experimental and clinical studies, a comprehensive and updated review

Saadat

Aslani

Ghorani

et al. 2021

Phytotherapy Research

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Nigella sativa (N. sativa) seed had been used traditionally due to several pharmacological effects. The updated experimental and clinical effects of N. sativa and its constituents on respiratory, allergic and immunologic disorders are provided in this comprehensive review article. Various databases including PubMed, Science Direct and Scopus were used. The preventive effects of N. sativa on pulmonary diseases were mainly due to its constituents such as thymoquinone, thymol, carvacrol and alpha-hederin. Extracts and constituents of N. sativa showed the relaxant effect, with possible mechanisms indicating its bronchodilatory effect in obstructive pulmonary diseases. In experimental animal models of different respiratory diseases, the preventive effect of various extracts and constituents of N. sativa was demonstrated by mechanisms such as antioxidant, immunomodulatory and antiinflammatory effects.Bronchodilatory and preventive effects of the plant and its components on asthma, COPD and lung disorders due to exposure to noxious agents as well as on allergic and immunologic disorders were also shown in the clinical studies. Various extracts and constituents of N. sativa showed pharmacological and therapeutic effects on respiratory, allergic and immunologic disorders indicating possible remedy effect of that the plant and its effective substances in treating respiratory, allergic and immunologic diseases.

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“…Tq is also shown to be effective as an antioxidant and anti-inflammatory agent (Gali-Muhtasib, Roessner, & Schneider-stock, 2016). Along those lines, this compound is shown to have protect effects against cigarette smoke-induced lung damage associated with COPD (Yetkin et al, 2020).…”

Section: Thymoquinone (Tq) Is An Active Component Isolated From Nigellamentioning

confidence: 99%

Thymoquinone (Tq) protects necroptosis induced by autophagy/mitophagy‐dependent oxidative stress in human bronchial epithelial cells exposed to cigarette smoke extract (CSE)

et al. 2020

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Chronic obstructive pulmonary disease (COPD) is characterized by cigarette smoke‐induced emphysema. Herein, we demonstrate protective effects of Thymoquinone (Tq), an active constituent from Nigella sativa, against cigarette smoke extract (CSE)‐induced abnormalities in bronchial epithelial cells. Dose‐dependent reduction in cell viability was observed in BEAS‐2B cells when exposed to different CSE concentrations, which was significantly reversed by Tq evident by LDH release. Levels of SOD, CAT, GR, GSH, and mitochondrial membrane ATPases were significantly reduced upon CSE exposure, an event, again, antagonized in presence of Tq. Similarly, Tq treatment significantly blocked CSE‐induced 4HNE elevations. Further, Tq‐improved mitochondrial dysfunction caused by CSE and significantly decreased autophagy/mitophagy markers like LC3II and p‐Drp. Tq also reduced necroptosis markers such as p‐MLKL, RIP‐1, and RIP‐3, by stabilizing PINK‐1 levels. In summary, Tq possesses protective properties against human bronchial epithelial cell autophagy/mitophagy‐dependent necroptosis caused by CSE, which warrants considerable attention for further preclinical evaluations. Practical applications This study demonstrates Thymoquinone (Tq), a natural plant extract to possess protective properties against human bronchial epithelial cell autophagy/mitophagy‐dependent necroptosis caused by cigarette smoke extract. The demonstrated efficacy of Tq will throw light for further preclinical evaluation of this molecule in CSE‐mediated complications. A detailed in vivo research is recommended.

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The protective effects of thymoquinone on lung damage caused by cigarette smoke

Cited by 13 publications

References 30 publications

Protective Effects of Thymoquinone, an Active Compound of Nigella sativa, on Rats with Benzo(a)pyrene-Induced Lung Injury through Regulation of Oxidative Stress and Inflammation

Protective Effects of Thymoquinone, an Active Compound of Nigella sativa, on Rats with Benzo(a)pyrene-Induced Lung Injury through Regulation of Oxidative Stress and Inflammation

The effects of Nigella sativa on respiratory, allergic and immunologic disorders, evidence from experimental and clinical studies, a comprehensive and updated review

Thymoquinone (Tq) protects necroptosis induced by autophagy/mitophagy‐dependent oxidative stress in human bronchial epithelial cells exposed to cigarette smoke extract (CSE)

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