The protective effects of vitamin C on the DNA damage, antioxidant defenses and aorta histopathology in chronic hyperhomocysteinemia induced rats

Boyacıoğlu, Murat; Sekkin, Selim; Kum, Cavit; Korkmaz, Deniz; Kiral, Funda; Yalinkilinc, Hande Sultan; Ak, Mehmet Onur; Akar, Ferda

doi:10.1016/j.etp.2014.06.004

Cited by 20 publications

(12 citation statements)

References 57 publications

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“…Other potential mechanisms of CAT activity reduction in HHcy can inhibit CAT activity by nitrite oxide, which can bind reversibly to the ferric iron . Regarding the possible mechanisms of HHcy, different antioxidant agents have been evaluated for the prevention of HHcy in many trials, such as resveratrol and other polyphenols, vitamin C, and folic acid …”

Section: Discussionmentioning

confidence: 99%

Vitamin B complex mitigates cardiac dysfunction in high‐methionine diet‐induced hyperhomocysteinemia

Jeremić

Turnic

Živković

et al. 2018

Clin Exp Pharma Physio

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This research is designed to test the hypothesis that elevated homocysteine (Hcy) levels in vivo, caused by a deficit in vitamin B complex, promote changes in cardiac function and redox status that lead to heart failure. In order to conduct the study, we used adult male Wistar albino rats (n = 30; 4 weeks old; 100 ± 15 g body weight). Hyperhomocysteinaemia (HHcy) in these animals was achieved by dietary manipulation. For 4 weeks, the animals were fed with a standard rodent chow (control, CF), a diet enriched in methionine with no deficiency in B vitamins (i.e., folic acid, B6 and B12) (HMNV) or a diet enriched in methionine and deficient in B vitamins (HMLV). After 28 days of dietary manipulation, all animals were killed. The rat hearts were isolated and retrogradely perfused according to the Langendorff technique at a gradually increasing perfusion pressure. We found a negative correlation between elevated serum Hcy and total body and heart weight. The maximum rate of left ventricular pressure development was significantly increased in the HMNV group compared with in the other groups. Systolic left ventricular pressure was significantly changed in all groups. HHcy induces remodelling of the cardiac tissues, as moderate HHcy is associated with more prominent interstitial and perivascular fibrosis. Our results suggest that a high methionine diet without vitamin B complex causes profound negative effects associated with HHcy.

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Section: Discussionmentioning

confidence: 99%

Vitamin B complex mitigates cardiac dysfunction in high‐methionine diet‐induced hyperhomocysteinemia

Jeremić

Turnic

Živković

et al. 2018

Clin Exp Pharma Physio

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“…Vitamin C (VitC) may protect against the negative effects of oxidative stress and apoptosis [29–36]. Therefore, in the present study, we assessed the interaction between isoflurane and VitC on caspase-3 activation, ROS accumulation, mitochondrial function and ATP levels, in order to further illustrate the up-stream mechanisms and the down-stream consequences of isoflurane-induced caspase-3 activation.…”

Section: Discussionmentioning

confidence: 99%

“…Vitamin C (VitC) has been shown to inhibit oxidative stress and apoptosis [29–36]. We therefore assessed in our studies whether or not VitC could attenuate the isoflurane-induced capase-3 activation through a ROS-, mitochondria- and ATP-associated mechanism in cultured cells, and whether VitC could also ameliorate the associated isoflurane-induced cognitive impairment seen in rodents.…”

Section: Introductionmentioning

confidence: 99%

Vitamin C Attenuates Isoflurane-Induced Caspase-3 Activation and Cognitive Impairment

et al. 2014

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Background Anesthetic isoflurane has been reported to induce caspase-3 activation. The underlying mechanism(s) and targeted intervention(s), however, remain largely to be determined. Vitamin C (VitC) inhibits oxidative stress and apoptosis. We therefore employed VitC to further determine the up-stream mechanisms and the down-stream consequences of the isoflurane-induced caspase-3 activation. Methods H4 human neuroglioma cells over expressed human amyloid precursor protein (H4-APP cells) and rat neuroblastoma cells were treated either with: 1) 2% isoflurane or 2) the control condition, plus saline or 400 mM VitC for three or six hours. Western blot analysis and fluorescence assay was utilized at the end of the experiments to determine caspase-3 activation, levels of reactive oxygen species and ATP, and mitochondrial function. The interaction of isoflurane (1.4% for two hours) and VitC (80 mg/kg) on cognitive function in mice was also assessed in the Fear Conditioning System. Results Here we show for the first time that the VitC treatment attenuated the isoflurane-induced caspase-3 activation. Moreover, VitC mitigated the isoflurane-induced increase in the levels of reactive oxygen species, opening of mitochondrial permeability transition pore, reduction in mitochondrial membrane potential, and the reduction in ATP levels in the cells. Finally, VitC ameliorated the isoflurane-induced cognitive impairment in the mice. Conclusion Pending confirmation from future studies, these results suggested that VitC attenuated the isoflurane-induced caspase-3 activation and cognitive impairment by inhibiting the isoflurane-induced oxidative stress, mitochondrial dysfunction, and reduction in ATP levels. These findings would promote further research into the underlying mechanisms and targeted interventions of anesthesia neurotoxicity.

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“…It has been reported that in the quantitative determination of DNA damage using the comet method, olive tail moment and the percent tail DNA are the most commonly used parameters [21] . In the present study, as in previous research, olive tail moment and the percent tail DNA were used as indicators of DNA damage [22,23] . The significant increase in the aforementioned parameters in the CP-only group compared to the groups in which CP was administered together with SLY and CUR suggests that CP has a marked genotoxic effect on DNA, and it was concluded that both SLY and CUR may be used reliably against the genotoxic effect of CP in cases where it has to be used in high doses.…”

Section: Discussionmentioning

confidence: 99%

“…The comet assay used here was adapted from the method described previously [21][22][23] . For this purpose, fresh blood samples were mixed with the PBS solution for the determination of DNA fragmentation of blood lymphocytes.…”

Section: Lymphocyte Isolation and Dna Analysis By Comet Assaymentioning

confidence: 99%

Ratlarda Deneysel Siklofosfamid Toksikasyonunda Silimarin ve Curcuminin Koruyucu ve Antigenotoksik Etkileri

Avcı¹,

Sekkin²,

Boyacıoğlu³

et al. 2016

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the protective effects of silymarin (SLY) and curcumin (CUR), which have strong antioxidant properties, against the toxic effects of high dose of cyclophosphamide (CP) on the liver and kidneys of rats. For this purpose, a total of 36 adult Wistar albino female rats at the weight of, 250±10 g were used. Biochemical parameters were found to be significantly higher in the CP-only group than in the other groups. In the groups in which SLY and CUR were administered concurrently with CP, biochemical parameters were found to be significantly lower than in the CP-only group. In the SLY+CP and CUR+CP groups, biochemical parameters were correlated with the severity of histopathological findings. In genotoxic examination, the comet parameter levels in the SLY+CP and CUR+CP-treated groups were lower than those in the CP-treated group. The present study established that both SLY and CUR are effective against the toxic effects of high doses of CP. Keywords

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The protective effects of vitamin C on the DNA damage, antioxidant defenses and aorta histopathology in chronic hyperhomocysteinemia induced rats

Cited by 20 publications

References 57 publications

Vitamin B complex mitigates cardiac dysfunction in high‐methionine diet‐induced hyperhomocysteinemia

Vitamin B complex mitigates cardiac dysfunction in high‐methionine diet‐induced hyperhomocysteinemia

Vitamin C Attenuates Isoflurane-Induced Caspase-3 Activation and Cognitive Impairment

Ratlarda Deneysel Siklofosfamid Toksikasyonunda Silimarin ve Curcuminin Koruyucu ve Antigenotoksik Etkileri

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