The protective potential of a<i>Fraxinus xanthoxyloides</i>ethyl acetate fraction against CCl<sub>4</sub>-induced oxidative stress in the cardiac tissue of rats

Jabeen, Faiza; Jafri, Laila; Rasul, Azhar; Manzoor, Maleeha; Shaheen, Muneeba; Riaz, Ammara

doi:10.1039/c9ra08729j

Cited by 7 publications

(6 citation statements)

References 41 publications

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“…On the contrary, the high dosage of FXE at 300 mg/kg and silymarin exhibited a protective efficacy comparable to that of the control after CCl 4 administration. At both doses administered, FXE did not induce any histological alterations in cardiac tissues, indicating that it does not have a discernible impact on normal tissue morphology [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Fraxin in Combination with Dexamethasone Attenuates LPS-Induced Liver and Heart Injury and Their Anticytokine Activity in Mice

Shaker,

Sahib

2023

Advances in Virology

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Background. Cytokine storm syndrome (CSS) is a major cause of morbidity and mortality in people suffering from hyperinflammatory status, which diverse etiological factors, including pathogens, therapeutic interventions, malignancies, and autoimmune disorders, can instigate. Since there is limited research on the antioxidant properties of fraxin and no studies have investigated its potential as an anticytokine storm agent, it is important to note that most studies have primarily focused on proinflammatory cytokines such as IL-1β, IL-6, and TNFα during cytokine storm. However, little research discusses the role of chemokines, particularly IL-8, during cytokine storms. Therefore, further investigation is warranted into the role of fraxin as an anticytokine storm agent and the involvement of IL-8 in cytokine storms. The present study examines the preventive efficacy of fraxin and the combination of fraxin and dexamethasone (FD) in mitigating lipopolysaccharide-induced systemic inflammation in mice caused by Escherichia coli, 055: B5. Methods. Five groups of ten mice were randomly assigned: LPS only group (5 mg/kg, intraperitoneally i.p.), control (normal saline N.S. 1 ml/kg, i.p.), concentrations were selected based on previous literature, fraxin (120 mg/kg, i.p.), dexamethasone (5 mg/kg, i.p.), fraxin + dexamethasone (FD) (60 mg/kg + 2.5 mg/kg, i.p.), administered one hour before LPS injection (5 mg/kg,i.p.), animals were euthanized next day, and interleukin-8 (IL-8) was quantified in serum using an enzyme-linked immunosorbent assay. The liver and heart tissues underwent histopathological analysis to assess morphological changes. For data analysis using ANOVA and Tukey post hoc tests, the Kruskal–Wallis and Mann–Whitney U tests were employed to analyze the histological results. Results. A significant decline in IL-8 levels was recorded in the treatment groups almost to the same degree ( p < 0.001 ), and the percentage of inhibition of IL-8 for fraxin, dexamethasone, and FD was 93%.92.4%, and 93%, respectively, compared to the LPS-only group. Histopathological scores were significantly reduced in liver and heart tissue ( P < 0.05 ). Conclusions. All interventions used in this study significantly reduced interleukin-8 (IL-8) levels and reduced LPS-induced liver and cardiac damage. The combination (FD) did not result in an evident superiority of either agent. More research is required to identify the possible usefulness of these agents in treating hyperinflammatory diseases, such as cytokine storms, in future clinical practice.

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Section: Discussionmentioning

confidence: 99%

Fraxin in Combination with Dexamethasone Attenuates LPS-Induced Liver and Heart Injury and Their Anticytokine Activity in Mice

Shaker,

Sahib

2023

Advances in Virology

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“…High performance liquid chromatography (HPLC‐DAD) analysis of the bark crude methanol extract (FXBM) of F. xanthoxyloides plant was achieved using HPLC‐DAD (Agilent 1200, Germany) fitted out with Zorbex RXC8 analytical column with 5 μm particle size and 25 ml capability using earlier described technique of Elansary et al [65] . Among the standards, ferulic acid, catechin, chlorogenic acid, gallic acid, caffeic acid, quercetin, and vanillic acid were analyzed at L368 nm Younis et al [37] . The HPLC‐DAD technique was performed in triplicate and the column was restored for 10 min after each run.…”

Section: Methodsmentioning

confidence: 99%

“…[35] Its vegetative parts (leaves and root bark) are traditionally used for jaundice, pneumonia, and malaria. [36,37] The chloroform fraction of F. xanthoxyloides bark extract exhibited anti-inflammatory activity in the air pouch model induced by carrageenan through reducing inflammatory mediators' levels including nitric oxide (NO), tissue necrosis factor (TNF)-α, interleukin (IL)-6, and prostaglandin E2 (PGE2). [38] The crude extract of Fraxinus angustifolia bark showed significant antioxidative potentials due to the presence of high polyphenolic content.…”

Section: Introductionmentioning

confidence: 99%

“…The aqueous extract from the stem bark is applied locally to relieve labor pain and bone fractures [35] . Its vegetative parts (leaves and root bark) are traditionally used for jaundice, pneumonia, and malaria [36,37] . The chloroform fraction of F. xanthoxyloides bark extract exhibited anti‐inflammatory activity in the air pouch model induced by carrageenan through reducing inflammatory mediators’ levels including nitric oxide (NO), tissue necrosis factor (TNF)‐α, interleukin (IL)‐6, and prostaglandin E2 (PGE2) [38] .…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl₄‐Induced Toxicity in Rats

Jabeen

Hussain

Rasool

et al. 2023

Chemistry & Biodiversity

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Fraxinus xanthoxyloides is a perennial shrub belonging to family Oleaceae, traditionally used for malaria, jaundice, pneumonia, inflammation, and rheumatism. Our study is aimed to assess the total phenolics (TPC), flavonoids (TFC), terpenoids contents (TTC) and antioxidant profiling of F. xanthoxyloides methanol bark extract (FXBM) and its fractions, hexane, chloroform, ethyl acetate and aqueous, along with high-performance liquid chromatography with diode-array detection (HPLC-DAD). Further, the antioxidant and pulmonary protective potential was explored against carbon tetrachloride (CCl 4 )-induced CCl4-induced pulmonary tissue damage in rats. The highest TPC, TFC and TTC were found in FXBM (133.29 � 4.19 mg/g), ethyl acetate fraction (279.55 � 10.35 mg/g), and chloroform fraction (0.79 � 0.06 mg/g), respectively. The most potent antioxidant capacity was depicted by FXBM (29.21 � 2.40 μg/mg) and ethyl acetate fraction (91.16 � 5.51 μg/mg). The HPLC-DAD analysis revealed the predominance of gallic, chlorogenic, vanillic and ferulic acid in FXBM. The administration of CCl 4 induced oxidative stress, suppressed antioxidant enzymes' activities including catalase, peroxidase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, and glutathione reductase. Further, it increased thiobarbituric acid reactive substances (TBARS) and H 2 O 2 levels, induced DNA injuries and reduced the total protein and glutathione content in lung tissues. The treatment of rats with FXBM restored these biochemical parameters to the normal level. Moreover, the histopathological studies of lung tissues demonstrated that FXBM protected rats' lung tissues from oxidative damage restoring normal lung functions. Thus, F. xanthoxyloides bark extract is recommended as adjuvant therapy as protective agent for patients with lung disorders.

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“…This compound is commonly used in experimental research to induce oxidative stress in liver 7 and also other organs such as heart. 8 Antioxidants are compounds that can degrade and get rid of free radicals. In the body, antioxidants can be divided into enzymatic and non-enzymatic antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Rice brain administration reduces malondialdehyde (MDA) levels in the heart of carbon tetrachloride-induced rats

Dwirini Retno Gunarti,

Reni Paramita,

Syailendra Karuna Sugito

et al. 2024

World J. Bio. Pharm. Health Sci.

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Excessive production of free radicals proven associated with disease progression of coronary heart disease. Rice bran oil has a high content of antioxidants such as tocopherol, tocotrienol, and γ-oryzanol. This study aimed to determine the effect of rice bran oil administration on malondialdehyde (MDA) level as an oxidative stress biomarker in the heart of carbon tetrachloride (CCl4)-induced rats. Thirty stored rat heart tissue samples that were divided into six groups, consisted of untreated control group, CCl4-treated group (CCl), rice bran oil-treated before CCl4 induction groups with rice bran oil dosage of 0.5 ml (B1+CCl) and 1.5 ml (B2+CCl), and rice bran oil-treated after CCl4 induction groups with rice bran oil dosage of 0.5 ml (CCl+B1) and 1.5 ml (CCl+B2), were measured for their MDA levels using Wills modified method. The highest heart’s MDA levels were obtained in the group that only received carbon tetrachloride. The lowest heart’s MDA levels were found in the group given 1.5 ml of rice bran oil before being given carbon tetrachloride. Heart MDA levels in all groups given rice bran either before or after administration of carbon tetrachloride were lower than those in the group given only carbon tetrachloride. The lowest heart MDA found in the group that was administered by 1.5 ml/day of rice bran oil before carbon tetrachloride induction.

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The protective potential of aFraxinus xanthoxyloidesethyl acetate fraction against CCl₄-induced oxidative stress in the cardiac tissue of rats

Abstract: Secondary metabolites present in medicinal plants offer a golden opportunity to fight different ailments, such as cancer, infections, diabetes, neurodegenerative and cardiovascular diseases, etc.

Cited by 7 publications

References 41 publications

Fraxin in Combination with Dexamethasone Attenuates LPS-Induced Liver and Heart Injury and Their Anticytokine Activity in Mice

Fraxin in Combination with Dexamethasone Attenuates LPS-Induced Liver and Heart Injury and Their Anticytokine Activity in Mice

Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl₄‐Induced Toxicity in Rats

Rice brain administration reduces malondialdehyde (MDA) levels in the heart of carbon tetrachloride-induced rats

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The protective potential of aFraxinus xanthoxyloidesethyl acetate fraction against CCl4-induced oxidative stress in the cardiac tissue of rats

Abstract: Secondary metabolites present in medicinal plants offer a golden opportunity to fight different ailments, such as cancer, infections, diabetes, neurodegenerative and cardiovascular diseases, etc.

Cited by 7 publications

References 41 publications

Fraxin in Combination with Dexamethasone Attenuates LPS-Induced Liver and Heart Injury and Their Anticytokine Activity in Mice

Fraxin in Combination with Dexamethasone Attenuates LPS-Induced Liver and Heart Injury and Their Anticytokine Activity in Mice

Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl4‐Induced Toxicity in Rats

Rice brain administration reduces malondialdehyde (MDA) levels in the heart of carbon tetrachloride-induced rats

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Resources

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The protective potential of aFraxinus xanthoxyloidesethyl acetate fraction against CCl₄-induced oxidative stress in the cardiac tissue of rats

Antioxidant and Pulmonary Protective Potential of Fraxinus xanthoxyloides Bark Extract against CCl₄‐Induced Toxicity in Rats