The protective role of chelators and antioxidants on mancozeb-induced                 toxicity in rat hippocampal astrocytes

Tsang, MM; Trombetta, Louis D.

doi:10.1177/0748233708089039

Cited by 29 publications

(16 citation statements)

References 33 publications

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“…Phase-contrast microscopy of treated cells showed distortion of cell shape, a decreased cell number, cytoplasmic vacuolization, floating cells, and cellular debris when compared with the controls. These observations were similar in appearance as presented in a study with another dithiocarbamate, Mancozeb (Tsang and Trombetta, 2007).…”

Section: Discussionsupporting

confidence: 87%

Exposure of rat hippocampal astrocytes to Ziram increases oxidative stress

Matei

Trombetta

2013

Toxicol Ind Health

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Pesticides have been shown in several studies to be the leading candidates of environmental toxins and may contribute to the pathogenesis of several neurodegenerative diseases. Ziram (zinc-bis(dimethyldithiocarbamate)) is an agricultural dithiocarbamate fungicide that is used to treat a variety of plant diseases. In spite of their generally acknowledged low toxicity, dithiocarbamates are known to cause a wide range of neurobehavioral effects as well as neuropathological changes in the brain. Astrocytes play a key role in normal brain physiology and in the pathology of the nervous system. This investigation studied the effects of 1.0 µM Ziram on rat hippocampal astrocytes. The thiobarbituric acid reactive substance assay performed showed a significant increase in malondialdehyde, a product of lipid peroxidation, in the Ziram-treated cells. Biochemical analysis also revealed a significant increase in the induction of 70 kDa heat shock and heme oxygenase 1 stress proteins. In addition, an increase of glutathione peroxidase (GPx) and a significant increase in oxidized glutathione (GSSG) were observed in the Ziram-treated cells. The ratio GSH to GSSG calculated from the treated cells was also decreased. Light and transmission electron microscopy supported the biochemical findings in Ziram-treated astrocytes. This data suggest that the cytotoxic effects observed with Ziram treatments may be related to the increase of oxidative stress.

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Section: Discussionsupporting

confidence: 87%

Exposure of rat hippocampal astrocytes to Ziram increases oxidative stress

Matei

Trombetta

2013

Toxicol Ind Health

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“…Mancozeb exposure was strongly associated with increased incidence of thyroid disease in pesticide applicators (Trivedi et al, 1993;Goldner et al, 2010). Reports are available on the toxicity of mancozeb on liver and kidney (Ksheerasagar and Kiliwal, 2003), on central nervous system (Nordby et al, 2005;Tsang and Trombetta, 2007). Histological studies of the testis of the mice treated with high doses of mancozeb revealed spermatogenesis inhibition (Ksheerasagar and Kaliwal, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of mancozeb-treated lettuce (<i>Lactuca sativa</i>) on wistar rat liver

Adjrah¹,

Karou²,

Agbonon³

et al. 2013

Ethiop. J. Env Stud & Manag

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Vegetable contamination by pesticides presents current problem of public health. Previous studies have shown that 60% vegetables sampled collected in Lomé (Togo) have pesticide residues exceeding the tolerable limits. Because mancozeb, ethylene bis-dithiocarbamate, is mainly used, more than five times, during the growth of lettuce (Lactuca sativa), the aim of the present investigation is to evaluate the potential effect of mancozeb-treated lettuce on the rat liver physiology. Mancozeb-treated lettuce and two doses of mancozeb were administered during 28 days to rats. Along the study, animal behavior was assessed, and at the end of administration, some hepatic enzymes such as transaminases and alkaline phosphatase were studied. The decrease in rat body weight was noted and animals have soft feces. Plasmatic concentrations of transaminases, alkaline phosphatase, and total bilirubin are increased in rats administered with mancozeb-treated lettuce. The plasmatic concentration of total protein is not decreased significantly. Those results indicate that lettuce collected directly from gardens, without washing or less washed, are not fit for human consumption.

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“…They found that significant cells death was caused by 250 and 500 μM MnCl 2 exposure for 24 h. However, Yin et al [21] found that the peaking of MTT reduction and LDH leakage occurred between Mn exposure for 2 h and 6 h that was different with our exposure time period. In addition, mancozeb, manganese ethylene-bis-dithiocarbamate with zinc salts, was observed to cause cytotoxicity on the cultured astrocytes [22]. It was also shown that exposure to Mn at a concentration of either 500 μM or 1 mM Mn elicited a dose-dependent increase in the LDH release of the N9 microglial cells [23].…”

Section: Discussionmentioning

confidence: 95%

G0/G1 Phase Arrest and Apoptosis Induced by Manganese Chloride on Cultured Rat Astrocytes and Protective Effects of Riluzole

Deng

et al. 2011

Biol Trace Elem Res

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Occupational or environmental exposure to excessive Mn would cause manganism, which is resembled Parkinson disease. However, the mechanism underlying manganism is still unknown. It had been documented that astrocytes play important roles in physiological function in brain. Therefore, in the present study, the cultured astrocytes were exposed to 0, 125, 250, and 500 μM MnCl(2), and cell viability, lactate dehydrogenase (LDH) leakage, morphological change, cell cycle progression, and apoptosis were determined. In addition, 100 μM riluzole (a glutamatergic modulator) was pretreated for 6 h before no MnCl(2) exposure or 500 μM MnCl(2) exposure. The results showed that cell viability inhibited, LDH leakage elevated, morphology injured, G(0)/G(1) phase cell cycle arrested, and apoptosis rate increased in a concentration-dependent manner. Further investigation indicated that riluzole pretreatment reversed cytotoxicity, cell cycle aberration, and apoptosis on astrocytes caused by MnCl(2). These results suggested that MnCl(2) could cause cytotoxicity, cell cycle arrest, and apoptosis concentration-dependently; riluzole might antagonize Mn toxicity on astrocytes.

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The protective role of chelators and antioxidants on mancozeb-induced toxicity in rat hippocampal astrocytes

Cited by 29 publications

References 33 publications

Exposure of rat hippocampal astrocytes to Ziram increases oxidative stress

Exposure of rat hippocampal astrocytes to Ziram increases oxidative stress

Effect of mancozeb-treated lettuce (<i>Lactuca sativa</i>) on wistar rat liver

G0/G1 Phase Arrest and Apoptosis Induced by Manganese Chloride on Cultured Rat Astrocytes and Protective Effects of Riluzole

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