The protective role of curcumin in human dental pulp stem cells stimulated by lipopolysaccharide via inhibiting NF-κB p65 phosphorylation to suppress NLRP3 inflammasome activation

Lan, Chunhua; Qian, Yueming; Wang, Yumin; Chen, Yuemin; Lin, Chensheng; Zhang, Yanding; Huang, Xiaojing

doi:10.1007/s00784-023-04885-8

Cited by 5 publications

(5 citation statements)

References 48 publications

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“…Some of the anti-inflammatory properties of curcumin are through induction of apoptosis in inflammatory cells and shortening of the inflammatory phase to promote healing. Curcumin has also been shown to promote cutaneous would healing [55] and has antiinflammatory roles in human dental pulp stem cells [56]. Other reported curcumin properties are collagen synthesis, fibroblasts migration, and differentiation [57][58][59][60][61][62].…”

Section: Discussionmentioning

confidence: 99%

Exploring the Therapeutic Potential of Curcumin for Periodontal Regeneration

2024

Dent Adv Res

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Periodontitis is a common inflammatory condition that results in destruction of tooth supporting tissues. Studies have demonstrated the regenerative potential of mesenchymal stem cells and biomaterials that induce healing and promotion of repair of the periodontal tissue.Recently, natural compounds have been identified as good candidates for drug development due to their immunomodulatory and healing properties. Specifically, curcumin has been shown to have anti-cancer, antibiotic and anti-inflammatory functions and has been reported safe and efficient for treatment of many diseases. In this study we investigate curcumin and ones of its metabolites, tetrahydrocurcumin, to identify a potential natural candidate that can contribute to the healing of periodontal tissue and promote its regeneration.In this study, human PDL and Gingival epithelial cells were treated with curcumin and tetrahydrocurcumin. Using RNA sequencing we investigated the pathways that were affected in these cell types. We show that curcumin and tetrahydrocurcumin promote Wnt signaling pathway, upregulate TGF-beta regulation of extracellular matrix. Curcumin promotes organization of extracellular matrix in PDL cells and cell migration, differentiation, and angiogenesis in gingival epithelial cells.The results of this study suggest that curcumin and its metabolite exhibit anti-inflammatory and have regenerative potential on PDL and gingival epithelial cell. Particularly, curcumin can be a low-cost, well-tolerated and effective natural candidate for therapeutic approaches in periodontal tissue. Further in vivo screening of this compound is required to confirm the optimum biological activity and to harness its full potential in clinical application.

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Section: Discussionmentioning

confidence: 99%

Exploring the Therapeutic Potential of Curcumin for Periodontal Regeneration

2024

Dent Adv Res

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“…One potential mechanism through which this downregulation may occur is by inhibiting the activation of NF-kB. Several studies have reported that natural compounds can exert their anti-inflammatory effects by modulating NF-kB activity (Song et al, 2018;Semmarath et al, 2022;Lan et al, 2023). These compounds can interfere with the suppression of NF-kB nuclear translocation.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory drug development focusing on immune mediated diseases

Monteiro,

Prasad,

Bhatti

et al. 2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Section: Discussionmentioning

confidence: 99%

Suppression of inflammation-induced lung cancer cells proliferation and metastasis by exiguaflavanone A and exiguaflavanone B from Sophora exigua root extract through NLRP3 inflammasome pathway inhibition

Arjsri,

Srisawad,

Semmarath

et al. 2023

Front. Pharmacol.

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Objective: Non-small cell lung cancer (NSCLC) is recognized for its aggressive nature and propensity for high rates of metastasis. The NLRP3 inflammasome pathway plays a vital role in the progression of NSCLC. This study aimed to investigate the effects of S. exigua extract and its active compounds on NLRP3 regulation in NSCLC using an in vitro model.Methods:S. exigua was extracted using hexane, ethyl acetate and ethanol to obtain S. exigua hexane fraction (SE-Hex), S. exigua ethyl acetate fraction (SE-EA), and S. exigua ethanol fraction (SE-EtOH) respectively. The active compounds were identified using column chromatography and NMR analysis. A549 cells were primed with lipopolysaccharide (LPS) and adenosine triphosphate (ATP) for activated NLRP3 inflammasome. The anti-inflammatory properties were determined using ELISA assay. The anti-proliferation and anti-metastasis properties against LPS-ATP-induced A549 cells were determined by colony formation, cell cycle, wound healing, and trans-well migration and invasion assays. The inflammatory gene expressions and molecular mechanism were determined using RT-qPCR and Western blot analysis, respectively.Results: SE-EA exhibited the greatest anti-inflammation properties compared with other two fractions as evidenced by the significant inhibition of IL-1β, IL-18, and IL-6, cytokine productions from LPS-ATP-induced A549 cells in a dose-dependent manner (p < 0.05). The analysis of active compounds revealed exiguaflavanone A (EGF-A) and exiguaflavanone B (EGF-B) as the major compounds present in SE-EA. Then, SE-EA and its major compound were investigated for the anti-proliferation and anti-metastasis properties. It was found that SE-EA, EGF-A, and EGF-B could inhibit the proliferation of LPS-ATP-induced A549 cells through cell cycle arrest induction at the G0/G1 phase and reducing the expression of cell cycle regulator proteins. Furthermore, SE-EA and its major compounds dose-dependently suppressed migration and invasion of LPS-ATP-induced A549 cells. At the molecular level, SE-EA, EGF-A, and EGF-B significantly downregulated the mRNA expression of IL-1β, IL-18, IL-6, and NLRP3 in LPS-ATP-induced A549 cells. Regarding the mechanistic study, SE-EA, EGF-A, and EGF-B inhibited NLRP3 inflammasome activation through suppressing NLRP3, ASC, pro-caspase-1(p50 form), and cleaved-caspase-1(p20 form) expressions.Conclusion: Targeting NLRP3 inflammasome pathway holds promise as a therapeutic approach to counteract pro-tumorigenic inflammation and develop novel treatments for NSCLC.

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The protective role of curcumin in human dental pulp stem cells stimulated by lipopolysaccharide via inhibiting NF-κB p65 phosphorylation to suppress NLRP3 inflammasome activation

Cited by 5 publications

References 48 publications

Exploring the Therapeutic Potential of Curcumin for Periodontal Regeneration

Exploring the Therapeutic Potential of Curcumin for Periodontal Regeneration

Anti-inflammatory drug development focusing on immune mediated diseases

Suppression of inflammation-induced lung cancer cells proliferation and metastasis by exiguaflavanone A and exiguaflavanone B from Sophora exigua root extract through NLRP3 inflammasome pathway inhibition

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