The protective role of l-Carintine and vitamin E on gamma irradiated rat’s tongue mucosa

Abstract

Background:The aim of this study was to investigate the antioxidant effect of L-carnitine (LC) against gamma-irradiation-induced oxidative damage in tongue of albino rats after total body irradiation with a single dose of 6 Gy. Materials and Methods: 48 adult rats were randomly divided into 3 groups of 16 animals each. Group I was irradiated with a single dose of 6 Gy. Group II received a daily i.p. injection of LC (250 mg/kg, i.p.) for 5 consecutive days and 1 h after the last dose, rats were irradiated with a single dose (6 Gy). Group III received a daily i.p. injection of LC (250 mg/kg, i.p.) and Vitamin E 40 mg/kg intramuscular daily for 5 consecutive days and 1 h after the last dose, rats were irradiated with a single dose (6 Gy). At day 7 and day 14 after treatment exposure, 8 rats from each group were sacrificed. Results: Administration of LC resulted in attenuation of the histological changes noticed in irradiated rats. The number of p53 positive nuclei significantly decreased in rats receiving LC alone or in combination with VE. Conclusion: LC and VE has shown positive effect in minimizing the epithelial atrophy of tongue mucosa after radiotherapy, which was emphasized by decreasing apoptotic activity in these tissues.

Ameliorative Effect of L-Carnitine against Histopathological Changes Induced by Methotrexate on the Lingual Mucosa of Albino Rats

“…It maintained an almost normal thickness of keratinous and epithelial layers of the lingual mucosa with subside of inflammatory signs. This is consistent with previous findings of El-Rouby et al, 27 and İpek et al 28 Carnitine derivatives are essential for glucose and lipid metabolism in cells and indicate cellular resistance to toxic effects. 29 LC is an effective antioxidant mediator.…”

Radioprotective potential of whey protein against gamma irradiation-induced lingual damage

“…These results agreed with Nguyen et al (2012) who reported that, within 1–3 days of irradiation, taste progenitor cells experience cell cycle arrest or apoptosis. This could be explained by the extreme radiosensitivity of the taste buds and taste nerves ( El-Rouby and El-Batouti, 2021 ).…”

“…2) In the WP group, rats received freshly prepared WP daily at a dose of 0.5 g/kg b.w., orally ( Jahovic et al, 2005 ) for 14 days (days 0–13). 3) In the IR group, animals were exposed to two doses of whole-body radiation; the first irradiation of 6 Gy on day 4 and the second irradiation of 3 Gy on day 6, with a total of 9 Gy accumulative dose, which was determined according to El-Rouby and El-Batouti, (2021) with some modifications. 4) In the WP + IR group, rats received a daily dose of WP (0.5 g/kg b.w., orally) for 14 days (days 0–13), prior and post-irradiation IR exposures.…”

Vitamin E ameliorates oral mucositis in gamma-irradiated rats (an in vivo study)

“…In accordance with our finding, Yeoh et al [ 35 ] found that P53 expression extraordinarily increased in irradiated rats specimens other than untreated controls. Besides, El-Rouby and El-Batouti [ 36 ] showed that P53 considerably reduced in rats treated with 40 mg/kg vitamin E for five consecutive days. Inconsistent with our results, P53 increased significantly in rats that were given vitamin E compared to other groups as revealed by Dashtiyan et al [ 37 ]; however, this may be due to giving vitamin E for 6 weeks or due to high lipid levels as the authors demonstrated.…”