The protective role of melatonin in citalopram-induced reproductive toxicity via modulating nitro-oxidative stress and apoptosis in male mice

Moradi, Mojtaba; Hashemian, Mohammad Arshia; Douhandeh, Erfan; Peysokhan, Mohsen; Hashemian, Amir Hossein; Faramarzi, Azita

doi:10.1016/j.reprotox.2023.108368

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…Numerous reports suggest that oxidative stress induces apoptosis, and antioxidants protect against apoptosis in noncancerous cells (Moradi et al, 2023;Sengul et al, 2023;Ijaz et al, 2023). OT could enhance testosterone secretion and decrease vacuolization in the germinal epithelium.…”

Section: Discussionmentioning

confidence: 99%

The protective effect of Ozone on the mice testicular damage induced by methotrexate

Khorsandi,

Varaa,

Dadfar

et al. 2024

JBRA

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Objective: Methotrexate (MTX) is widely administered for the treatment of various cancers. However, MTX induces male reproductive toxicity. In the current study, the effect of ozone therapy (OT) on reducing the toxic effects of MTX in the mouse testicles has been investigated.Methods: Twenty-four mice were divided into four groups: control, OT (4 mg/kg ozone), MTX (20 mg/kg), and MTX + OT. Testosterone levels, histological changes, and oxidative stress biomarkers were assessed to evaluate the protective effects of OT.Results: The results demonstrated that MTX disrupted germinal epithelium, reduced serum testosterone levels, and enhanced oxidative stress in testicular tissue. However, treatment with OT attenuated these adverse effects. OT effectively restored the levels of antioxidant enzymes, such as catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD). OT reduced lipid peroxidation, as indicated by decreased malondialdehyde (MDA) levels. OT preserved normal spermatogenesis, improved morphometric parameters, and reduced histological changes by MTX. Moreover, OT effectively restored testosterone levels.Conclusions: OT protects against MTX-induced testicular damage by suppressing oxidative stress.

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Section: Discussionmentioning

confidence: 99%

The protective effect of Ozone on the mice testicular damage induced by methotrexate

Khorsandi,

Varaa,

Dadfar

et al. 2024

JBRA

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“…For the histopathological investigation, one tissue section of each testis was selected and assessed for possible changes in the organization of germinal epithelium and interstitial connective tissue following CTL and Vit-C administration. [6,34]…”

Section: Histopathological Examinationmentioning

confidence: 99%

“…The following formula was used to calculate the percentage of apoptotic cells: A mean number of positively stained apoptotic cells/ mean the total number of the cells × 100%. [6] 2. process. Accordingly, TUNEL staining was performed to confirm the apoptotic process in the testicular tissue.…”

Section: Detection Of Apoptic Cells By Tunel and Dapi Stainingmentioning

confidence: 99%

“…[3,4] However, there is growing concern about the potential adverse effects of CTL on the reproductive and endocrine systems. [2,5,6] Indeed, accordingly, SSRIs are well known for affecting male reproductive performance. [2,3,5] Like other SSRIs, CTL contributes to sexual dysfunction, including decreased libido, arousal, orgasm, and erectile dysfunction.…”

Section: Introductionmentioning

confidence: 99%

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Utility of vitamin C in ameliorating citalopram‐induced testicular toxicity via modulating nitro‐oxidative stress and apoptosis in mice

Moradi,

Hashemian,

Fathi

et al. 2023

J Biochem & Molecular Tox

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There is a growing concern that antidepressant drugs impair sexual function and adversely impact spermatogenesis and male fertility. Vitamin C is a natural antioxidant that plays a vital role in the male reproductive system. The present study investigated the ameliorating potential of vitamin C against citalopram (CTL)‐evoked testicular toxicity and spermatogenesis impairment in mice. Mice were randomly divided into six groups: control, CTL, vitamin C 100, vitamin C 200, CTL plus vitamin C 100, and CTL plus vitamin C 200. Adult male mice were intraperitoneally (ip) injected with 10 mg/kg of CTL for 35 days with or without vitamin C. At the end of the study, body and testes weight, sperm parameters, histopathology of testes, testosterone level, testicular levels of malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (TAC), and apoptosis (TUNEL assay) were evaluated. Our findings revealed that vitamin C restored spermatogenesis by improving sperm count, motility, viability, morphology, and chromatin integrity. Testosterone levels and testes histopathology were significantly improved in the vitamin C‐administrated groups. Furthermore, vitamin C administration markedly alleviated CTL‐induced nitro‐oxidative damage, enhancing TAC levels, and reducing NO and MDA levels. Whilst CTL therapy induced a significant increase in the number of TUNEL‐positive cells compared to the control, the administration of vitamin C significantly prevented the apoptotic effects of CTL. Together, vitamin C therapy protects against CTL‐induced testicular damage via mitigating nitro‐oxidative stress and apoptosis, which provides evidence for vitamin C as a beneficial therapy against antidepressant drug‐associated reproductive toxicity and male sub/infertility.

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Carvacrol showed a curative effect on reproductive toxicity caused by Bisphenol AF via antioxidant, anti-inflammatory and anti-apoptotic properties

Uyar

Cellat

Kanat

et al. 2023

Reproductive Toxicology

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The protective role of melatonin in citalopram-induced reproductive toxicity via modulating nitro-oxidative stress and apoptosis in male mice

Cited by 4 publications

References 48 publications

The protective effect of Ozone on the mice testicular damage induced by methotrexate

The protective effect of Ozone on the mice testicular damage induced by methotrexate

Utility of vitamin C in ameliorating citalopram‐induced testicular toxicity via modulating nitro‐oxidative stress and apoptosis in mice

Carvacrol showed a curative effect on reproductive toxicity caused by Bisphenol AF via antioxidant, anti-inflammatory and anti-apoptotic properties

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