The protective roles of zinc and estradiol in renal ischemia/reperfusion injury in ovariectomized rats

Barekat, Foroogh; Talebi, Ardeshir; Nematbakhsh, Mehdi

doi:10.15171/jnp.2018.21

Cited by 8 publications

(7 citation statements)

References 24 publications

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“…Role of Zn 2+ in the activation of phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway to protect against renal I/ R injury has been investigated in published research (10). It has been observed that I/R injury results in significant increase in the serum levels of blood urea nitrogen (BUN) and creatinine and increase in the fractional excretion of sodium (FENa) which is decreased by zinc chloride (ZnCl 2 ) supplementation (11,12). Besides, Zn 2+ also improved glomerular filtration rate value, Cr-clearance, and urine flow rate beyond renal I/R induced renal injury (12,13).…”

Section: Introductionmentioning

confidence: 99%

TRPC6 ameliorates renal ischemic reperfusion injury by inducing Zn2+ influx and activating autophagy to resist necrosis

Pu¹,

Zhao²,

Shen³

et al. 2022

Ann Transl Med

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Background: Renal ischemic reperfusion injury (RIRI) is the most hackneyed cause of acute renal injury with high incidence. As a slit diaphragm (SD), TRPC6 (transient receptor potential channel 6) can maintain the structure and function of glomerular podocytes, and its activation has been reported to prominently alleviate ischemia reperfusion (I/R). However, the specific mechanism of TRPC6 in RIRI is uncertain.Methods: The TRPC6 specific shRNA or overexpressing plasmid was used to decrease or increase TRPC6 level in HK-2 cells, respectively. Subsequently, the OGD/R (oxygen-glucose deprivation and re-oxygenation) HK-2 cells and RIRI model rats was established to examine the effect of TRPC6 in RIRI in vitro. After processing, viability was confirmed with MTT; cell necrosis was analyzed with flow cytometry; necrosis and autophagy-related proteins were verified with Western blot; free Zn 2+ was tested with an Zn 2+ fluorescent probe; and cell autophagy was monitored with MDC (monodansylcadaverine) method. Furthermore, TRPC6 agonist (OGA) or TRPC6 inhibitor (SKF96365) were introduced to increase or inhibit the activity of TRPC6 in RIRI model rats, and the kidney injury was assessed with H&E staining and RIP1 and PARP-1 expressions were examined with IHC (immunohistochemistry) staining.Results: Our results verified TRPC6 could markedly enhance viability, Zn 2+ influx, and autophagy, and suppressed necrosis in OGD/R HK-2 cells. In addition, increase of Zn 2+ or autophagy activation produced similar results to TRPC6 overexpression in viability, autophagy, and necrosis of OGD/R HK-2 cells. Rescue experiment results also showed TRPC6 could prevent necrosis and facilitate Zn 2+ influx and autophagy of OGD/R HK-2 cells by inducing Zn 2+ influx and autophagy. Moreover, TRPC6 could ameliorate kidney injury, block necrosis, and enhance autophagy in RIRI model rats by promoting Zn 2+ influx and autophagy.Conclusions: TRPC6 could prevent necrosis and induce autophagy to alleviate RIRI by accelerating Zn 2+ influx and autophagy. This shows TRPC6/Zn 2+ influx/autophagy might be a novel therapeutic strategy for RIRI.

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Section: Introductionmentioning

confidence: 99%

TRPC6 ameliorates renal ischemic reperfusion injury by inducing Zn2+ influx and activating autophagy to resist necrosis

Pu¹,

Zhao²,

Shen³

et al. 2022

Ann Transl Med

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“…Zinc supplementation (10 mg/kg/day; 3 weeks) also improved renal function in the ischemia/reperfusion injury model in ovariectomized rats. An improvement in serum blood urea nitrogen and creatinine concentrations was found [ 22 ]. O’Kane et al [ 23 ] investigated the renoprotective effect of Zn 2+ (doses of 0.1, 0.5, 1.0, 2.5, 10.0 mg/kg) preconditioning in a clinically relevant sheep model of IRI.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

2021

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Zinc is an effective anti-inflammatory and antioxidant trace element. The aim of this study was to analyse the protective effect of zinc and zinc–prolactin systems as additives of preservation solutions in the prevention of nephron damage caused during ischemia. The study used a model for storing isolated porcine kidneys in Biolasol®. The solution was modified with the addition of Zn at a dose of 1 µg/L and Zn: 1 µg/L with prolactin (PRL): 0.1 µg/L. After 2 h and 48 h of storage, the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, sodium, potassium, creatinine and total protein were determined. Zinc added to the Biolasol® composition at a dose of 1 µg/L showed minor effectiveness in the protection of nephrons. In turn, Zn2+ added to Biolasol + PRL (PRL: 0.1 µg/L) acted as a prolactin inhibitor. We do not recommend the addition of Zn(II) (1 µg/L) and Zn(II) (1 µg/L) + PRL (0.1 µg/L) to the Biolasol solution.

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“…Zn as a protective agent against RIR was studied before, and it was shown that Zn improved RIR injury (14, 24, 25). Possibly, the antioxidant effect of Zn (26) is the major cause of this protection since Zn increases metallothionein production, which scavenges free radicals (OH) (27) and Zn deficiency disturbs lipid peroxidation system (28).…”

Section: Discussionmentioning

confidence: 99%

“…Possibly, the antioxidant effect of Zn (26) is the major cause of this protection since Zn increases metallothionein production, which scavenges free radicals (OH) (27) and Zn deficiency disturbs lipid peroxidation system (28). Barekat and colleagues reported that Zn protected the kidney against RIR in ovariectomised rats (24) while the protective role of Zn against RIR injury reported to be sex-related (29). On the contrary, co-treatment of Zn and IPC did not protect the kidney against RIR.…”

Section: Discussionmentioning

confidence: 99%

Zinc Supplementation and Ischemia Pre-conditioning in Renal Ischemia/Reperfusion Injury

Mazaheri

Emami

Moslemi

et al. 2019

MJMS

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Backgrounds Renal ischemia/reperfusion (RIR) is a major cause of kidney dysfunction in clinic. The main objective of this study was to investigate the effect of pre-conditioning ischemia (IPC) and zinc (Zn) supplementation on renal RIR injury. Methods A total of 63 unilateral nephrectomised male and female Wistar rats were divided into five groups. Group 1 (ShOPR): Rats as sham-operated group were subjected to surgical procedure without RIR. Group 2 (Isch): Rats underwent RIR (left kidney ischemia for 30 min followed by 48 h reperfusion). Group 3 (Zn+Isch): Rats were treated as group 2 but they received Zn sulphate (30 mg/kg) 1 h before induction of RIR. Group 4 (IPC+Isch): Rats were treated as group 2 but they underwent 1 min of ischemia followed by 3 min reperfusion as IPC, which was repeated for three times before induction of RIR. Group 5 (Zn+IPC+Isch): Rats were subjected to receive both Zn sulphate and IPC before induction of RIR. Urine samples were collected in the last 6 h of reperfusion, and finally biochemical and histological measurements were performed. Results The serum level of creatinine (Cr), normalised kidney weight (KW) and kidney tissue damage score (KTDS) increased by RIR alone significantly ( P < 0.05). These parameters were attenuated statistically by Zn supplementation ( P < 0.05). However, IPC alone or co-treatment of Zn and IPC did not improve the biochemical and histological markers altered by RIR injury. Conclusion Zn supplementation had a protective role against RIR while such protective effect was not observed by IPC alone or by co-treatment of Zn and IPC.

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The protective roles of zinc and estradiol in renal ischemia/reperfusion injury in ovariectomized rats

Cited by 8 publications

References 24 publications

TRPC6 ameliorates renal ischemic reperfusion injury by inducing Zn2+ influx and activating autophagy to resist necrosis

TRPC6 ameliorates renal ischemic reperfusion injury by inducing Zn2+ influx and activating autophagy to resist necrosis

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

Zinc Supplementation and Ischemia Pre-conditioning in Renal Ischemia/Reperfusion Injury

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