The protein component of scrapie-associated fibrils is a glycosylated low molecular weight protein.

Abstract

Scrapie-associated fibril protein (SAF-protein) (Figure 1). All preparations contained one major protein in the region of 26 kd with a smearing component in the range below 25 kd.

Detection of Scrapie-associated Fibril (SAF) Proteins Using Anti-SAF Antibody in Non-purified Tissue Preparations

“…These results and those reported in this paper indicate that post-translational modifications together with structural changes of the host protein confer PK resistance. The SAF protein is known to be glycosylated Manuelidis et al, 1985 ;Multhaup et al, 1985; R. J. Kascsak et al, unpublished).…”

Chemoprophylaxis of scrapie in mice

“…The aggregation into SAF is the most specific (Merz et al, 1984;Bode et al, 1985;Farquhar et al, 1989), as well as a pathogenetically very important (Diringer et al, 1988), step in the development of clinical symptoms in all the unconventional virus diseases. After biochemical concentration (Multhaup et al, 1985), the SAF protein can be sensitively detected by antisera raised against the fibril protein . Therefore, we performed an immunoblot analysis on the individual mouse brains to confirm our results based on clinical diagnosis.…”

“…Therefore, we performed an immunoblot analysis on the individual mouse brains to confirm our results based on clinical diagnosis. SAF amyloid was isolated by a time-saving modification of a standard procedure (Multhaup et al, 1985). Brain homogenate (5 ml, 10~) was processed to pellet P22 as published (Multhaup et al, 1985).…”

“…SAF amyloid was isolated by a time-saving modification of a standard procedure (Multhaup et al, 1985). Brain homogenate (5 ml, 10~) was processed to pellet P22 as published (Multhaup et al, 1985). This pellet was dissolved by sonication in 4 ml Tris-buffered saline containing 19/00 of the detergent sulphobetain 3,14.…”

Characterization of Antisera against Scrapie-associated Fibrils (SAF) from Affected Hamster and Cross-reactivity with SAF from Scrapie-affected Mice and from Patients with Creutzfeldt-Jakob Disease

“…No reaction was obtained with the preimmune sera. The three mouse sera raised against SAF from hamster brain as formic acid-denatured SAF protein (Multhaup et al, 1985) also reacted specifically in this test. Typical NC-ELISA results with one rabbit and one mouse serum are shown in Fig.…”