The protein encoded by the gene proliferation disrupter (prod) is associated with the telomeric retrotransposon array in Drosophila melanogaster

Török, Tibor; Benitez, Cecil M.; Takács, S; Biessmann, Harald

doi:10.1007/s00412-006-0090-4

Cited by 23 publications

(27 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The second approach could involve isolating proteins that bind to the telomeric transposons in vivo. Several such proteins have been identified, which could serve as positive controls (Andreyeva et al 2005;Török et al 2007). The challenge here is to functionally separate capping factors from those that regulate transcription and transposition of the elements.…”

Section: Challenges For the Telomere Field In Drosophilamentioning

confidence: 99%

Telomere capping in Drosophila: dealing with chromosome ends that most resemble DNA breaks

Rong

2008

Chromosoma

View full text Add to dashboard Cite

Telomere caps prevent chromosome ends from being recognized as DNA double-strand breaks (DSBs). Unlike most organisms studied, the telomere-capping function of Drosophila does not require a specific sequence. Without this sequence component, Drosophila telomeres most resemble DNA breaks and, thus, represent a simpler system for the study of telomere capping. I review recent progress in Drosophila telomere studies, and challenge the notion that Drosophila may not be a relevant model for the study of telomere maintenance.

show abstract

Section: Challenges For the Telomere Field In Drosophilamentioning

confidence: 99%

Telomere capping in Drosophila: dealing with chromosome ends that most resemble DNA breaks

Rong

2008

Chromosoma

View full text Add to dashboard Cite

show abstract

“…Of those that have, the Ku70/Ku80 complex, a component of NHEJ, and the E(TC) protein limit chromosome elongation via gene conversion (53,66). PROD binds just upstream of the HeT-A promoter, and prod mutants exhibit increased HeT-A transcript levels, suggesting that PROD acts as a repressor of HeT-A transcriptional activity (67). However, given that prod mutants do not develop long HTT arrays, it appears that increased transcript levels are not sufficient to drive increased transposition of these elements.…”

Section: The Retrotransposon Array and Chromosome Elongationmentioning

confidence: 99%

“…Proteins associated with the HTT array, however, are difficult to identify, because no genetic screen can be performed for HTT-binding proteins owing to the absence of a predictable mutant phenotype. Therefore, immunohistological staining for potential candidate proteins is one of the few options, which so far has identified three proteins at the HTT array: Z4, JIL-1 and PROD (44,67). All three proteins are also associated with a multitude of other sites in the Drosophila genome, suggesting general and widespread functions ( Table 2).…”

Section: Proteins Associated With the Htt Arraymentioning

confidence: 99%

Drosophila telomeres: an exception providing new insights

2007

Self Cite

View full text Add to dashboard Cite

SummaryDrosophila telomeres are comprised of DNA sequences that differ dramatically from those of other eukaryotes. Telomere functions, however, are similar to those found in telomerase-based telomeres, even though the underlying mechanisms may differ. Drosophila telomeres use arrays of retrotransposons to maintain chromosome length, while nearly all other eukaryotes rely on telomerase-generated short repeats. Regardless of the DNA sequence, several end-binding proteins are evolutionarily conserved. Away from the end, the Drosophila telomeric and subtelomeric DNA sequences are complexed with unique combinations of proteins that also modulate chromatin structure elsewhere in the genome. Maintaining and regulating the transcriptional activity of the telomeric retrotransposons in Drosophila requires specific chromatin structures, and while telomeric silencing spreads from the terminal repeats in yeast, the source of telomeric silencing in Drosophila is the subterminal arrays. However, the subterminal arrays in both species may be involved in telomere-telomere associations and/or communication.

show abstract

“…The PROD protein localizes strongly to the centric heterochromatin of the second and third chromosomes, to telomeres and to >400 euchromatic sites (Török et al, 1997). In the HTT array, PROD binds to a region just upstream of the HeT-A promoter (Török et al, 2007). Heterozygous prod mutants exhibit elevated levels of HeT-A transcript in ovaries, suggesting that prod may be involved in the control of telomere elongation as a repressor of HeT-A transcription, although it is without a demonstrated role in telomere capping.…”

Section: Hp1 and Prod Modulate Transcription Of Telomeric Retroelementsmentioning

confidence: 99%

Regulation of telomere length in <i>Drosophila</i>

Frydrychová

Biessmann

Mason

2008

Cytogenet Genome Res

View full text Add to dashboard Cite

Telomeres in all organisms must perform the same vital functions to ensure cell viability: to act as a protective chromosome cap that distinguishes natural chromosome ends from DNA double strand breaks, and to balance the loss of DNA from the chromosome end due to incomplete DNA replication. Most eukaryotes rely on a specialized reverse transcriptase, telomerase, to generate short repeats at the chromosome end to maintain chromosome length. Drosophila, however, uses retrotransposons that target telomeres. Transposition of these elements may be controlled by small RNAs and spreading of silent chromatin from the telomere associated sequence, both of which limit the retrotransposon expression level. Proteins binding to the retrotransposon array, such as HP1 and PROD, may also modulate transcription. It is not clear however, that simply increasing transcript levels of the telomeric retrotransposons is sufficient to increase transposition. The chromosome cap may control the ability of the telomere-specific elements to attach to chromosome ends. As in other organisms, chromosomes can be elongated by gene conversion. Although the mechanism is not known, HP1, a component of the cap, and the Ku proteins are key components in this pathway.

show abstract

The protein encoded by the gene proliferation disrupter (prod) is associated with the telomeric retrotransposon array in Drosophila melanogaster

Cited by 23 publications

References 69 publications

Telomere capping in Drosophila: dealing with chromosome ends that most resemble DNA breaks

Telomere capping in Drosophila: dealing with chromosome ends that most resemble DNA breaks

Drosophila telomeres: an exception providing new insights

Regulation of telomere length in <i>Drosophila</i>

Contact Info

Product

Resources

About