The protein kinase TOUSLED is required for maintenance of transcriptional gene silencing in <i>Arabidopsis</i>

Wang, Yu; Li, Jun; Ran, Xia; Wang, Junguo; Shen, Jie; Cao, Rui; Hong, Xuhui; Zhu, Jian‐Kang; Gong, Zhizhong

doi:10.1038/sj.embor.7400852

Cited by 51 publications

(63 citation statements)

References 31 publications

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“…In addition, as mentioned earlier, in Arabidopsis, ASF1B is phosphorylated by the TOUSLED protein kinase (Ehsan et al, 2004). Arabidopsis tls mutants are highly sensitive to genotoxic stress, including UV-B radiation (Wang et al, 2007). Even more, in humans, Sen and De Benedetti (2006) provided evidence that TLK1B protects against UV radiation via Asf1-mediated chromatin assembly at the sites of UV damage.…”

Section: Discussionmentioning

confidence: 98%

ANTI-SILENCING FUNCTION1 Proteins Are Involved in Ultraviolet-Induced DNA Damage Repair and Are Cell Cycle Regulated by E2F Transcription Factors in Arabidopsis

et al. 2013

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ANTI-SILENCING FUNCTION1 (ASF1) is a key histone H3/H4 chaperone that participates in a variety of DNA-and chromatin-related processes, including DNA repair, where chromatin assembly and disassembly are of primary relevance. Information concerning the role of ASF1 proteins in the post-ultraviolet (UV) response in higher plants is currently limited. In Arabidopsis (Arabidopsis thaliana), an initial analysis of in vivo localization of ASF1A and ASF1B indicates that both proteins are mainly expressed in proliferative tissues. In silico promoter analysis identified ASF1A and ASF1B as potential targets of Elongation Factor2 (E2F) transcription factors. These observations were experimentally validated, both in vitro, by electrophoretic mobility shift assays, and in vivo, by chromatin immunoprecipitation assays and expression analysis using transgenic plants with altered levels of different E2F transcription factors. These data suggest that ASF1A and ASF1B are regulated during cell cycle progression through E2F transcription factors. In addition, we found that ASF1A and ASF1B are associated with the UV-B-induced DNA damage response in Arabidopsis. Transcript levels of ASF1A and ASF1B were increased following UV-B treatment. Consistent with a potential role in UV-B response, RNA interference-silenced plants of both genes showed increased sensitivity to UV-B compared with wild-type plants. Finally, by coimmunoprecipitation analysis, we found that ASF1 physically interacts with amino-terminal acetylated histones H3 and H4 and with acetyltransferases of the Histone Acetyl Transferase subfamily, which are known to be involved in cell cycle control and DNA repair, among other functions. Together, we provide evidence that ASF1A and ASF1B are regulated by cell cycle progression and are involved in DNA repair after UV-B irradiation.

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Section: Discussionmentioning

confidence: 98%

ANTI-SILENCING FUNCTION1 Proteins Are Involved in Ultraviolet-Induced DNA Damage Repair and Are Cell Cycle Regulated by E2F Transcription Factors in Arabidopsis

et al. 2013

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“…This suggests possible regulatory interactions involving these genes that require BRM-mediated chromatin remodelling. TSL was recently shown to be involved in chromatin modiWcations (Wang et al 2007). Interestingly, the BRAHMA SWI/SNF CRC in Drosophila melanogaster was found to act together with a histone chaperone ASF1, which is a target for a TSL-like kinase (Moshkin et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of functional redundancy of BRM ATPase and ATSWI3C subunits of Arabidopsis SWI/SNF chromatin remodelling complexes

Archacki

Sarnowski

Halibart-Puzio

et al. 2009

Planta

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In yeast and mammals, ATP-dependent chromatin remodelling complexes of the SWI/SNF family play critical roles in the regulation of transcription, cell proliferation, diVerentiation and development. Homologues of conserved subunits of SWI/SNF-type complexes, including Snf2-type ATPases and SWI3-type proteins, participate in analogous processes in Arabidopsis. Recent studies indicate a remarkable similarity between phenotypic eVects of mutations in the SWI3 homologue ATSWI3C and bromodomain-ATPase BRM genes. To verify the extent of functional similarity between BRM and ATSWI3C, we have constructed atswi3c brm double mutants and compared their phenotypic traits to those of simultaneously grown single atswi3c and brm mutants. In addition to inheritance of characteristic developmental abnormalities shared by atswi3c and brm mutants, some additive brm-speciWc traits were also observed in the atswi3c brm double mutants. Unlike atswi3c, the brm mutation results in the enhancement of abnormal carpel development and pollen abortion leading to complete male sterility. Despite the overall similarity of brm and atswi3c phenotypes, a critical requirement for BRM in the diVerentiation of reproductive organs suggests that its regulatory functions do not entirely overlap those of ATSWI3C. The detection of two diVerent transcript isoforms indicates that BRM is regulated by alternative splicing that creates an in-frame premature translation stop codon in its SNF2-like ATPase coding domain. The analysis of Arabidopsis mutants in nonsense-mediated decay suggests an involvement of this pathway in the control of alternative BRM transcript level.

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“…of the core DNA-replication proteins, including the DNA single-strand-binding protein RPA2A (Xia et al, 2006), Pola (Liu et al, 2010a), Pol « (Yin et al, 2009), POLD2 (this study), RFC1 (Liu et al, 2010b), and TOUSLED protein kinase (Wang et al, 2007). Other studies have identified other DNA replication-related proteins involved in mediating TGS, including BRU1 (Takeda et al, 2004), chromatin assembly factor (CAF-1) subunits FAS1 and FAS2 (Ono et al, 2006;Schonrock et al, 2006), and TEBICHI (Inagaki et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

The second subunit of DNA-polymerase delta is required for genomic stability and epigenetic regulation

et al. 2016

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ORCID IDs: 0000-0002-1641-8650 (J.Z.); 0000-0002-6719-9814 (S.X.); 0000-0001-5134-731X (J.-K.Z.).DNA polymerase d plays crucial roles in DNA repair and replication as well as maintaining genomic stability. However, the function of POLD2, the second small subunit of DNA polymerase d, has not been characterized yet in Arabidopsis (Arabidopsis thaliana). During a genetic screen for release of transcriptional gene silencing, we identified a mutation in POLD2. Whole-genome bisulfite sequencing indicated that POLD2 is not involved in the regulation of DNA methylation. POLD2 genetically interacts with Ataxia Telangiectasia-mutated and Rad3-related and DNA polymerase a. The pold2-1 mutant exhibits genomic instability with a high frequency of homologous recombination. It also exhibits hypersensitivity to DNA-damaging reagents and short telomere length. Whole-genome chromatin immunoprecipitation sequencing and RNA sequencing analyses suggest that pold2-1 changes H3K27me3 and H3K4me3 modifications, and these changes are correlated with the gene expression levels. Our study suggests that POLD2 is required for maintaining genome integrity and properly establishing the epigenetic markers during DNA replication to modulate gene expression.

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The protein kinase TOUSLED is required for maintenance of transcriptional gene silencing in Arabidopsis

Cited by 51 publications

References 31 publications

ANTI-SILENCING FUNCTION1 Proteins Are Involved in Ultraviolet-Induced DNA Damage Repair and Are Cell Cycle Regulated by E2F Transcription Factors in Arabidopsis

ANTI-SILENCING FUNCTION1 Proteins Are Involved in Ultraviolet-Induced DNA Damage Repair and Are Cell Cycle Regulated by E2F Transcription Factors in Arabidopsis

Genetic analysis of functional redundancy of BRM ATPase and ATSWI3C subunits of Arabidopsis SWI/SNF chromatin remodelling complexes

The second subunit of DNA-polymerase delta is required for genomic stability and epigenetic regulation

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