The protein map of the protozoan parasiteLeishmania (Leishmania) amazonensis,Leishmania(Viannia) braziliensisandLeishmania (Leishmania) infantumduring growth phase transition and temperature stress

Abstract

Leishmaniaparasites cause a spectrum of diseases termed leishmaniasis, which manifests in two main clinical forms, cutaneous and visceral leishmaniasis.Leishmaniapromastigotes transit from proliferative exponential to quiescent stationary phases inside the insect vector, a relevant step that recapitulates early molecular events of metacyclogenesis. During the insect blood meal of the mammalian hosts, the released parasites interact initially with the skin, an event marked by temperature changes. Deep knowledge on the molecular events activated duringLeishmania-host interactions in each step is crucial to develop better therapies and to understand the pathogenesis. In this study, the proteomes ofLeishmania(Leishmania) amazonensis(La),Leishmania(Viannia) braziliensis(Lb), andLeishmania(Leishmania) infantum(synL. L. chagasi)(Lc) were analyzed using quantitative proteomics to uncover the proteome modulation in three different conditions related to growth phases and temperature shifts: 1) exponential phase (Exp); 2) stationary phase (Sta25) and; 3) stationary phase subjected to heat stress (Sta34). Functional validations were performed using orthogonal techniques, focusing on α-tubulin, gp63 and heat shock proteins (HSPs). Species-specific and condition-specific modulation highlights the plasticity of theLeishmaniaproteome, showing that pathways related to metabolism and cytoskeleton are significantly modulated from exponential to stationary growth phases, while protein folding, unfolded protein binding, signaling and microtubule-based movement were differentially altered during temperature shifts. This study provides an in-depth proteome analysis of threeLeishmaniaspp., and contributes compelling evidence of the molecular alterations of these parasites in conditions mimicking the interaction of the parasites with the insect vector and vertebrate hosts.