The Proteins of Catecholamine‐storing Organelles

Winkler, Hans

doi:10.1111/j.1365-3083.1982.tb03759.x

Cited by 9 publications

(1 citation statement)

References 146 publications

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“…However, the addition of carbachol during the third and fourth chase periods (b, lanes 3 and 4) resulted in a dramatic stimulation of the secretion of some proteins (compare a, lane 3, with b, lane 3), for example those of Mr 39,000 and 78,000 (closed arrows). Although we have not identified these stimulated proteins, they are likely to include the chromogranins, which are soluble protein components ofchromaffin granules (29,41). In contrast, other proteins such as those of Mr 32,000 and 81,000 (open arrows) were secreted constitutively, i.e., their secretion was not increased by carbachol.…”

Section: Sorting Of Secreted Proteins Into Two Pathways In Pc 12 Cellsmentioning

confidence: 84%

Selective packaging of human growth hormone into synaptic vesicles in a rat neuronal (PC12) cell line.

Schweitzer¹,

Kelly²

1985

The Journal of Cell Biology

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We have introduced the gene for human growth hormone (hGH) into PC12 cells, a rat pheochromocytoma-derived cell line with neuronal characteristics, and have isolated stable cell lines that express this protein, hGH is stored within the cells in membrane-bounded vesicles that are indistinguishable from the endogenous catecholaminergic synaptic vesicles. When the transfected cells are stimulated by carbachol or direct depolarization, they release norepinephrine and hGH with parallel kinetics. Treatment of the transfected cells with nerve growth factor results in a twofold increase in the amounts of hGH stored in and secreted from the cells. Not all proteins are packaged into the synaptic vesicles, since the rate of release of laminin, a soluble secreted protein endogenous to PC12 cells, is not stimulated by carbachol. This neuronal cell line therefore possesses at least two distinct pathways for secretion and can selectively package a foreign endocrine hormone into the regulated pathway.

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Section: Sorting Of Secreted Proteins Into Two Pathways In Pc 12 Cellsmentioning

confidence: 84%