The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling

Schmidt, Juliane; Müsken, Mathias; Becker, Tanja; Magnowska, Zofia; Bertinetti, Daniela; Möller, Stefan; Zimmermann, Bastian; Herberg, Friedrich W.; Jänsch, Lothar; Häußler, Susanne

doi:10.1371/journal.pone.0018184

Cited by 62 publications

(54 citation statements)

References 62 publications

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“…In vitro methylation assays using membranes prepared from E. coli cells that express PctA and purified CheR1 from P. aeruginosa have demonstrated that PctA is methylated by CheR1 methyltransferase at a rate that is increased by attractant stimuli (Schmidt et al, 2011). However, those authors did not determine the sites at which the methylation occurred.…”

Section: Discussion Pctapp Signalling Abilities Within E Coli Complexesmentioning

confidence: 99%

A chemoreceptor from Pseudomonas putida forms active signalling complexes in Escherichia coli

2012

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Section: Discussion Pctapp Signalling Abilities Within E Coli Complexesmentioning

confidence: 99%

A chemoreceptor from Pseudomonas putida forms active signalling complexes in Escherichia coli

2012

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“…The diameter of bacterial colonies and the clear-zone diameter were measured with calipers (Schmidt et al, 2011).…”

Section: Measurement Of Colony Diameter and Diameter Of Clear Zonementioning

confidence: 99%

Isolation and identification of bacterial protease enzyme of leather waste

Pertiwiningrum

Anggraini

Fitrianto

et al. 2017

J. Indonesian Trop. Anim. Agric.

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ABSTRAKPenelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri penghasil enzim protease dari limbah cair dan padat penyamakan kulit, dan mengetahui karakterisasi aktivitas enzim yang dihasilkan. Isolasi bakteri menggunakan sampel limbah cair dan padat dari limbah penyamakan kulit yang diambil dari 4 penampungan limbah yang berbeda (tiga limbah cair dan 1 limbah padat dari fase unhairing). Data hasil isolasi bakteri, pengukuran pertumbuhan bakteri pada OD 600 nm, identifikasi bakteri dan pemekatan enzim 60% ammonium sulfat dianalisis secara deskriptif. Data uji diameter koloni, diameter zona bening, indeks proteolitik dan uji karakterisasi aktivitas enzim terhadap pH dan suhu yang berbeda-beda dianalisis dengan menggunakan analisis ragam, apabila terdapat perbedaan dilanjutkan dengan uji Duncan's New Multiple Range Test. Hasil isolasi bakteri sampel limbah cair dari kolam penampungan limbah yang kedua (L2) diuji lebih lanjut karena menunjukkan adanya aktivitas proteolitik. Identifikasi isolat bakteri L2 mempunyai morfologi koloni berbentuk bulat, berwarna putih, tepian rata dan elevasi cembung, morfologi sel berbentuk bacillus, warna merah, gram negatif, tidak motil, katalase positif, dan gelatin negatif. Aktivitas enzim tertinggi ditunjukkan pada pH 11 dengan aktivitas unit enzim 45.18±1.77 U/mL dan aktivitas spesifik enzim 43.19±1.69 U/mg dan suhu 40°C dengan aktivitas unit enzim 54.02±1.89 U/mL dan aktivitas spesifik enzim 51.65±1.8 U/mg. Aktivitas enzim dari protease yang terpresipitasi ammonium sulfat 60% menunjukkan hasil yang lebih tinggi (75.8 U/mL) daripada protease kasar. Kata kunci : Isolasi bakteri, enzim protease, limbah penyamakan kulit, Unhairing ABSTRACTThe objectives of this study were to isolate and identify bacteria which produced protease enzyme from liquid and solid waste of tannery, and their characterization of enzymatic activities. The bacterial isolation used a sample of liquid and solid waste from leather waste which taken from a different waste reservoirs (three liquid waste and one solid waste in unhairing phase). Data of the isolated bacteria, OD 600 nm bacterial growth, the identification of bacteria, and enzyme precipitation with 60% ammonium sulfate were analyzed descriptively. The colony diameter, diameter of clear zone, proteolytic index, and enzymatic activities characterization on difference of pH and temperature were analyzed using Completely Randomized Design, followed by Duncan's New Multiple Range Test. The second sample from four samples of the isolated bacteria was tested further for their proteolytic activity. The morphology of the colony was circle, white, flat ledges and convex elevation, the basal cell morphology was red, gram-negative, non-motile, catalase positive and gelatin negative. The highest activity of Protease Enzyme from Bacteria Isolate (A. Pertiwiningrum et al.) 33 enzyme on pH 11 with activity unit enzyme 45,18±1,77 U/ml and specific enzyme activity 43,19±1,69 U/mg and temperature of 40°C activity unit enzyme 54,02±1,89 U/ml and specif...

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“…Chemotaxis is required for outward migration of the swarming colony in V. parahaemolyticus and Vibrio alginolyticus (80,81), but not for any of the other bacteria examined to date. In Rhodospirillum centenum, B. subtilis, and P. aeruginosa, nonchemotactic mutants whose flagellar motors are CCW biased (expected to form flagellar bundles) can swarm, while those that are CW biased do not (82)(83)(84). Aberrant and nonswarming patterns have been reported for nonchemotactic mutants of P. mirabilis (85), but chemotaxis is apparently not required for swarming (86).…”

Section: Signaling Pathways That Control the Decision To Movementioning

confidence: 99%

Swarming: Flexible Roaming Plans

Partridge

Harshey

2012

Journal of Bacteriology

169

216

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Movement over an agar surface via swarming motility is subject to formidable challenges not encountered during swimming. Bacteria display a great deal of flexibility in coping with these challenges, which include attracting water to the surface, overcoming frictional forces, and reducing surface tension. Bacteria that swarm on "hard" agar surfaces (robust swarmers) display a hyperflagellated and hyperelongated morphology. Bacteria requiring a "softer" agar surface (temperate swarmers) do not exhibit such a dramatic morphology. For polarly flagellated robust swarmers, there is good evidence that restriction of flagellar rotation somehow signals the induction of a large number of lateral flagella, but this scenario is apparently not relevant to temperate swarmers. Swarming bacteria can be further subdivided by their requirement for multiple stators (Mot proteins) or a statorassociated protein (FliL), secretion of essential polysaccharides, cell density-dependent gene regulation including surfactant synthesis, a functional chemotaxis signaling pathway, appropriate cyclic (c)-di-GMP levels, induction of virulence determinants, and various nutritional requirements such as iron limitation or nitrate availability. Swarming strategies are as diverse as the bacteria that utilize them. The strength of these numerous designs stems from the vantage point they offer for understanding mechanisms for effective colonization of surface niches, acquisition of pathogenic potential, and identification of environmental signals that regulate swarming. The signature swirling and streaming motion within a swarm is an interesting phenomenon in and of itself, an emergent behavior with properties similar to flocking behavior in diverse systems, including birds and fish, providing a convenient new avenue for modeling such behavior.

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The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling

Cited by 62 publications

References 62 publications

A chemoreceptor from Pseudomonas putida forms active signalling complexes in Escherichia coli

A chemoreceptor from Pseudomonas putida forms active signalling complexes in Escherichia coli

Isolation and identification of bacterial protease enzyme of leather waste

Swarming: Flexible Roaming Plans

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