The PTS
            <sup>Ntr</sup>
            -KdpDE-KdpFABC Pathway Contributes to Low Potassium Stress Adaptation and Competitive Nodulation of Sinorhizobium fredii

Feng, Xue-Ying; Tian, Yu; Cui, Wen; Li, Yue-Zhen; Wang, Dan; Liu, Yanbo; Jiao, Jian; Chen, Wenxin; Tian, Chang Fu

doi:10.1128/mbio.03721-21

Cited by 9 publications

(3 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, in limiting K + conditions (< 5 mM), the unphosphorylated form of EIIA Ntr was shown to directly interact with KdpD and stimulate its kinase activity, thereby promoting phosphorylation of KdpE and the subsequent increase of kdp genes expression (Luttmann et al, 2009). This EIIA Ntr -based control of KdpD has also been reported in the plant symbionts Rhizobium leguminosarum (Untiet et al, 2013) and Sinorhizobium fredii (Feng et al, 2022), two a-proteobacteria closely related to C. crescentus. It was later discovered that EIIA Ntr regulates in fact sigma factor selectivity by modulating K + levels.…”

Section: Discussionmentioning

confidence: 72%

“…Potassium (K + ) is the most abundant monovalent cation within living cells (1,2) and it is essential to set the membrane potential and intracellular pH (2)(3)(4)(5). As K + also emerges as an important regulator of bacterial physiology in view of its role in diverse processes (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) including biofilm formation, chemotaxis, cell to cell communication or virulence, it is therefore expected that K + transport is tightly regulated. In bacteria, the KdpDE two-component system (TCS) responds to potassium depletion by regulating the expression of the cognate high-affinity K + transporter KdpFABC (18).…”

Section: Importance Introductionmentioning

confidence: 99%

“…Potassium (K + ) is the most abundant monovalent cation within living cells (Danchin & Nikel, 2019, Epstein, 2003 and it is essential to set the membrane potential and intracellular pH (Bakker & Mangerich, 1981, Booth, 1999, Epstein, 2003, Ochrombel et al, 2011. As K + also emerges as an important regulator of bacterial physiology in view of its role in diverse processes (Castaneda-Garcia et al, 2011, Dominguez-Ferreras et al, 2009, Feng et al, 2022, Gries et al, 2013, Humphries et al, 2017, Liu et al, 2013, MacGilvary et al, 2019, Prindle et al, 2015, Quintero-Yanes et al, 2019, Rasmussen, 2023, Su et al, 2009, Valente & Xavier, 2016 including biofilm formation, chemotaxis, cell to cell communication or virulence, it is therefore expected that K + transport is tightly regulated.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Regulation of potassium homeostasis inCaulobacter crescentus

Quintero-Yanes

Collignon

et al. 2023

Preprint

View full text Add to dashboard Cite

Potassium (K+) is an essential physiological element determining membrane potential, intracellular pH, osmotic/turgor pressure, and protein synthesis in cells. Nevertheless, K+homeostasis remains poorly studied in bacteria. Here we describe the regulation of potassium uptake systems in the oligotrophic α-proteobacteriumCaulobacter crescentusknown as a model for asymmetric cell division. We show that C. crescentus can grow in concentrations from the micromolar to the millimolar range by essentially using two K+transporters to maintain potassium homeostasis, the low affinity Kup and the high affinity Kdp uptake systems. When K+is not limiting, we found that thekupgene is essential whilekdpinactivation does not impact the growth. In contrast,kdpbecomes critical but not essential andkupdispensable for growth in K+-limited environments. However, in the absence ofkdp, mutations inkupwere selected to improve growth in K+-depleted conditions, likely by improving the affinity of Kup for K+. In addition, mutations in the KdpDE two-component system, which regulateskdpABCexpression, suggest that the inner membrane sensor regulatory component KdpD works as a kinase in early stages of growth and as a phosphatase to regulate transition into stationary phase. Our data also show that KdpE is not only phosphorylated by KdpD but also by another non-cognate histidine kinase. On top of this, we determined the KdpE-dependent and independent K+transcriptome as well as the direct targets of KdpE. Together, our work illustrates how an oligotrophic bacterium responds to fluctuation in K+availability.

show abstract

Section: Discussionmentioning

confidence: 72%

Section: Importance Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of potassium homeostasis inCaulobacter crescentus

Quintero-Yanes

Collignon

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Invasive plant species interact with drought to shift key functions and families in the native rhizosphere

Ettinger,

LaForgia

2023

Plant Soil

View full text Add to dashboard Cite

Background and aims Interactions between species invasions and climate change have the potential to drive changes in plant communities more than either factor alone. One pathway through which these effects can occur is via changes to the rhizosphere microbial community. Invasive plants can alter these microbial communities affecting natives’ abilities to compete with invaders. At the same time, climate change is leading to more frequent extreme wet and dry events. Understanding the response of plant communities to these combined global change drivers requires a comprehensive approach that assesses the relationship between plant competition and belowground rhizosphere microbial community responses. Methods Here we use a field experiment in a California grassland with a set of six native annual forbs (i.e., wildflowers) and three invasive annual grasses to test how competition with invasive plants alters both identity and function in the native rhizosphere microbiome, and whether competition between these groups interacts with rainfall to amplify or ameliorate microbial shifts. Results Metagenomics of rhizosphere communities revealed that drought combined with competition from invaders altered a higher number of functions and families in the native rhizosphere compared to invasive competition alone or drought alone. Watering combined with invasion led to fewer shifts. Conclusion This suggests invasion-driven shifts in the microbial community may be involved in weakening natives’ ability to cope with climate change, especially drought. Understanding the role of the microbial community under invasion and climate change may be critical to mitigating the negative effects of these interacting global change drivers on native communities. Graphical abstract Understanding plant community response to global change drivers requires a comprehensive approach that assesses the relationship between plant competition and belowground rhizosphere microbial community responses. (a) In this work, we use a field experiment in a California grassland with a set of native forbs (purple) and invasive grasses (teal) to assess the combined effects of competition and water availability (drought, control, watered) on the rhizosphere microbiome. (b) Drought combined with competition from invaders altered the relative abundance of 36 functions (white) and 22 microbial families (blue) in the native rhizosphere compared to the effects of competition (3 functions, 16 families) or drought alone on natives (not shown: 5 functions, 0 families). (c) Additionally, regardless of watering treatment, invasive grasses sourced more of the taxonomic community in native-invasive mixes and this was exacerbated during drought. Overall, these results suggest invasion-driven shifts in the microbiome may be involved in weakening natives’ ability to cope with climate change, especially drought.

show abstract

The evolutionary ecology of rhizobia: multiple facets of competition before, during, and after symbiosis with legumes

Burghardt

diCenzo

2023

Current Opinion in Microbiology

View full text Add to dashboard Cite

The PTS ^Ntr -KdpDE-KdpFABC Pathway Contributes to Low Potassium Stress Adaptation and Competitive Nodulation of Sinorhizobium fredii

Abstract: In all ecological niches, potassium is actively consumed by diverse prokaryotes and their interacting eukaryote hosts. It is only just emerging that potassium is a key player in host-pathogen interactions, and the role of potassium in mutualistic interactions remains largely unknown.

Cited by 9 publications

References 54 publications

Regulation of potassium homeostasis inCaulobacter crescentus

Regulation of potassium homeostasis inCaulobacter crescentus

Invasive plant species interact with drought to shift key functions and families in the native rhizosphere

The evolutionary ecology of rhizobia: multiple facets of competition before, during, and after symbiosis with legumes

Contact Info

Product

Resources

About

The PTS Ntr -KdpDE-KdpFABC Pathway Contributes to Low Potassium Stress Adaptation and Competitive Nodulation of Sinorhizobium fredii

Abstract: In all ecological niches, potassium is actively consumed by diverse prokaryotes and their interacting eukaryote hosts. It is only just emerging that potassium is a key player in host-pathogen interactions, and the role of potassium in mutualistic interactions remains largely unknown.

Cited by 9 publications

References 54 publications

Regulation of potassium homeostasis inCaulobacter crescentus

Regulation of potassium homeostasis inCaulobacter crescentus

Invasive plant species interact with drought to shift key functions and families in the native rhizosphere

The evolutionary ecology of rhizobia: multiple facets of competition before, during, and after symbiosis with legumes

Contact Info

Product

Resources

About

The PTS ^Ntr -KdpDE-KdpFABC Pathway Contributes to Low Potassium Stress Adaptation and Competitive Nodulation of Sinorhizobium fredii