The pulse duration dependence of inertial cavitation dose and hemolysis

Brayman, Andrew A.; Chen, Wen-shiang; Matula, Thomas J.; Crum, L. A.; Miller, Morton W.

doi:10.1109/ultsym.2002.1192554

Cited by 2 publications

(3 citation statements)

References 5 publications

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“…Beyond acoustic onset pressures, the HIFU pulses caused the particles to cavitate consistently, giving a sonographic brightness comparable to that of commercial microbubbles (Figure S4). Additionally, an audible snapping noise was only observed in the presence of the acoustically active particles insonated with HIFU, signifying broadband acoustic emissions associated with inertial cavitation. ,, These observations, taken together, represent “cavitation” or “cavitation nucleation” for these studies: the growth of bubbles from the nanoparticle solution whose inherent compressibility and collapse allow for an effective sonographic visualization, as reported previously. ,, Typically, particles were tested within several minutes after dilution for each trial. Unless otherwise noted, displayed sonograms of the phantom were taken with an HIFU peak negative pressure of 10.8 MPa.…”

Section: Methodssupporting

confidence: 54%

“…Additionally, an audible snapping noise was only observed in the presence of the acoustically active particles insonated with HIFU, signifying broadband acoustic emissions associated with inertial cavitation. 30,41,42 These observations, taken together, represent "cavitation" or "cavitation nucleation" for these studies: the growth of bubbles from the nanoparticle solution whose inherent compressibility and collapse allow for an effective sonographic visualization, as reported previously. 18,19,29 Typically, particles were tested within several minutes after dilution for each trial.…”

Section: ■ Materials and Methodsmentioning

confidence: 66%

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Temperature-Responsive Hydrophobic Silica Nanoparticle Ultrasound Contrast Agents Directed by Phospholipid Phase Behavior

Blum

Yıldırım

Gyorkos

et al. 2019

ACS Appl. Mater. Interfaces

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In this paper, we report ultrasonically-active nanoscale contrast agents that behave as thermometric sensors through phase change in their stabilizing phospholipid monolayer. Phospholipid-stabilized, hydrophobic mesoporous silica nanoparticles (P@hMSNs) are known to interact with high intensity focused ultrasound (HIFU) to promote cavitation at their surfaces, which can be used for both imaging and therapy. We show that the lateral lipid phase behavior of the phosphocholine lipid dictates the acoustic contrast of the P@hMSNs. When the lipids are in gel phase below their melting temperature, the P@hMSNs generate detectable microbubbles when exposed to HIFU. However, if the lipids exhibit a liquid expanded phase, the P@hMSNs cease to generate bubbles in response to HIFU insonation. We verify that the heating and subsequent transition of lipid coating the hMSN is associated with the loss of acoustic response by doping laurdan dye into the lipid monolayer and imaging lipid phase through red shifts in emission spectra. Similarly, cessation of cavitation was also induced by adding a fluidizing surfactant such as Triton X, which could be reversed upon washing away excess surfactant. Finally, by controlling for the partial fluidization caused by the adsorption of protein, P@hMSNs may be used as thermometric sensors of bulk fluid temperature. These findings not only impact the utilization of nanoscale agents as stimulusresponsive ultrasound contrast agents, but also have broader implications for how cavitation may be initiated at surfaces coated by a surfactant.

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Section: Methodssupporting

confidence: 54%

Section: ■ Materials and Methodsmentioning

confidence: 66%

Temperature-Responsive Hydrophobic Silica Nanoparticle Ultrasound Contrast Agents Directed by Phospholipid Phase Behavior

Blum

Yıldırım

Gyorkos

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“… 44 - 46 Additionally, Crum and coworkers have reported on nuclei being present after microbubble destruction. 47 , 48 However, to our knowledge, no one has reported on the ability of microbubble debris itself being able to nucleate and grow in a manner that can then be visualized by ultrasound imaging, nor provided a plausible hypothesis to its mechanism.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles Formed by Acoustic Destruction of Microbubbles and Their Utilization for Imaging and Effects on Therapy by High Intensity Focused Ultrasound

Blum¹,

Yıldırım²,

Chattaraj³

et al. 2017

Theranostics

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This work reports that when PEG-lipid-shelled microbubbles with fluorocarbon interior (C4F10, C5F12, or C6F14) are subjected to ultrasound pulses, they produce metastable, fluid-filled nanoparticles that can be re-imaged upon administration of HIFU. The nanoparticles produced by destruction of the microbubbles (MBNPs) are of 150 nm average diameter and can be re-imaged for up to an hour after creation for C 4F10, and for at least one day for C5F12. The active species were found to be fluid (gas or liquid) filled nanoparticles rather than lipid debris. The acoustic droplet vaporization threshold of the nanoparticles was found to vary with the vapor pressure of the encapsulated fluorocarbon, and integrated image brightness was found to increase dramatically when the temperature was raised above the normal boiling point of the fluorocarbon. Finally, the vaporization threshold decreases in serum as compared to buffer, and administration of HIFU to the nanoparticles caused breast cancer cells to completely detach from their culture substrate. This work demonstrates a new functionality of microbubbles that could serve as a platform technology for ultrasound-based theranostics.

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The pulse duration dependence of inertial cavitation dose and hemolysis

Cited by 2 publications

References 5 publications

Temperature-Responsive Hydrophobic Silica Nanoparticle Ultrasound Contrast Agents Directed by Phospholipid Phase Behavior

Temperature-Responsive Hydrophobic Silica Nanoparticle Ultrasound Contrast Agents Directed by Phospholipid Phase Behavior

Nanoparticles Formed by Acoustic Destruction of Microbubbles and Their Utilization for Imaging and Effects on Therapy by High Intensity Focused Ultrasound

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