The purification and sequence analysis of an avian neuromedin U

Domin, Jan; Benito-Orfila, M.A.; Nandha, Kiran A.; Aitken, Alastair; Bloom, Stephen R.

doi:10.1016/0167-0115(92)90508-r

Cited by 36 publications

(20 citation statements)

References 14 publications

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“…This factor, however, does not diminish its significance, since biological potency is perhaps more important than absolute quantity. The concentration of this peptide in secretions is, however, significantly higher than the levels of NmU found in neuroendocrine source tissues from other species (rat spinal cord, 61.0 pmol/g (40); chicken intestine, 97.5 pmol/g (23); rabbit small intestine, 22 pmol/g (21); and pig spinal cord, 26.6 pmol/g (40)). These levels are somewhat lower, however, than those found in frog gut, which are reported to be as much as 10-fold higher but still considerably less than levels present in L. caerulea skin secretion (19).…”

Section: Discussionmentioning

confidence: 79%

“…Frog NmU-23 and rat NmU-23 are unique in that they lack this putative site, and therefore it is probable that they exist only as the elongated form. Conservation of Glu 5 and Gln 8 in the N-terminal region of porcine NmU-25 is found in the frog NmU-23, and the highly conserved Pro 10 is present in all other species except chicken (23). The N-terminal region is thought to increase the potency and prolong the activity of the peptide (15), and preservation of these residues is likely to be essential for biological activity.…”

Section: Discussionmentioning

confidence: 99%

“…The amino acid sequence for human neuromedin U can be accessed in the Swiss-Prot Database under accession no. P48645 (23). The amino acid sequence for rat neuromedin U can be accessed in the Swiss-Prot Database under accession no.…”

Section: Resultsmentioning

confidence: 99%

“…NmU immunoreactivity has been found throughout the peripheral and central nervous systems, with the highest concentrations in the pituitary gland, gastrointestinal tract, and nucleus accumbens (16). Primary structural information currently exists on six other members of this peptide family from the rat (17,18), frog (19), guinea pig (20), rabbit (21), dog (22), and chicken (23). The primary structure of a putative human NmU-25 has been deduced from cDNA encoding the human NmU precursor (24).…”

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confidence: 99%

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Isolation, Structural Characterization, and Bioactivity of a Novel Neuromedin U Analog from the Defensive Skin Secretion of the Australasian Tree Frog, Litoria caerulea

Salmon¹,

Johnsen²,

Bienert³

et al. 2000

Journal of Biological Chemistry

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We report the isolation of a novel bioactive peptide, neuromedin U-23 (NmU-23), from the defensive skin secretion of the Australasian tree frog, Litoria caerulea. The primary structure of the peptide was established by a combination of microsequencing, mass spectroscopy and site-directed antiserum immunoreactivity as SDEEVQVPGGVISNGYFLFRPRN-amide (M r 2580.6). A synthetic replicate of frog NmU-23 displaced monoradioiodinated rat NmU-23 from uterine membranes in a dose-dependent fashion indistinguishable from nonisotopically labeled rat NmU-23. In a rat uterine smooth muscle strip preparation, synthetic frog NmU-23 produced dose-dependent contractions identical to porcine NmU-25. However, in a preparation of human urinary bladder muscle strip, the synthetic frog peptide was more potent than porcine NmU-25 in eliciting contraction and produced desensitization of the preparation to the latter peptide. This report demonstrates that the defensive skin secretion of a frog contains a novel peptide exhibiting a high degree of primary structural similarity to the endogenous vertebrate peptide, NmU, and that this frog skin analog displays biological activity in mammalian tissues.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Isolation, Structural Characterization, and Bioactivity of a Novel Neuromedin U Analog from the Defensive Skin Secretion of the Australasian Tree Frog, Litoria caerulea

Salmon¹,

Johnsen²,

Bienert³

et al. 2000

Journal of Biological Chemistry

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“…Following the initial identification of porcine NmU, this peptide was purified and sequenced from rat Minamino et al, 1988), frog (Domin et al, 1989), rabbit (Kage et al, 1991), dog (O'Harte et al, 1991a), and chicken (O'Harte et al, 1991b;Domin et al, 1992). The amino acid sequences of these are shown in Fig.…”

Section: Discovery Isolation Structure and Synthesis Of Neuromementioning

confidence: 99%

Neuromedin U and Its Receptors: Structure, Function, and Physiological Roles

Brighton

Szekeres²,

Willars³

2004

Pharmacological Reviews

248

219

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Distribution of neuromedin U-like immunoreactivity in the central nervous system ofRana esculenta

et al. 1996

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The distribution of perikarya and nerve fibers containing neuromedin U-like immunoreactivity in the brain of Rana esculenta was determined with an antiserum directed toward the carboxyl terminus of the peptide. In the telencephalon, immunoreactive perikarya were found in the olfactory bulb, the medial septum, and the diagonal band. In the diencephalon, labeled perikarya were detected in the anterior and posterior preoptic areas, the dorsal nucleus of the hypothalamus, the caudal part of the infundibulum, and the posterior tuberculum. In the mesencephalon, immunoreactive cell bodies were found only in the laminar nucleus of the torus semicircularis and the anterodorsal tegmental nucleus. In the rhombencephalon, labeled perikarya were detected in the secondary visceral nucleus, the cerebellar nucleus, the central gray, and the nucleus of the solitary tract. Immunoreactive nerve fibers were observed in all areas of the brain that contained labeled perikarya. The densest accumulations were found in the nucleus accumbens; the dorsal part of the lateral septum; the periventricular region of the ventral thalamus; the lateral part of the infundibulum; the anterodorsal, anteroventral, posterodorsal, and posteroventral tegmental nuclei; and the periaqueductal region of the tegmentum. The distribution of neuromedin U-like immunoreactivity in the frog brain was substantially different from the distribution described for the rodent brain.

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The purification and sequence analysis of an avian neuromedin U

Cited by 36 publications

References 14 publications

Isolation, Structural Characterization, and Bioactivity of a Novel Neuromedin U Analog from the Defensive Skin Secretion of the Australasian Tree Frog, Litoria caerulea

Isolation, Structural Characterization, and Bioactivity of a Novel Neuromedin U Analog from the Defensive Skin Secretion of the Australasian Tree Frog, Litoria caerulea

Neuromedin U and Its Receptors: Structure, Function, and Physiological Roles

Distribution of neuromedin U-like immunoreactivity in the central nervous system ofRana esculenta

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